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Preparation method of self-assembled lysozyme multilayer membrane with antibacterial property and biocompatibility

A biocompatible, lysozyme technology, applied in coatings, pharmaceutical formulations, drug delivery, etc., to achieve the effect of simple and easy method, reduced damage, and broad application prospects

Inactive Publication Date: 2017-09-01
WENZHOU INST OF BIOMATERIALS & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In summary, there is currently no prior art literature disclosing a technical solution for self-assembling lysozyme and plant polyphenols using strong intermolecular hydrogen bonds to obtain a biomultilayer film with antibacterial properties and biocompatibility. improve and innovate

Method used

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  • Preparation method of self-assembled lysozyme multilayer membrane with antibacterial property and biocompatibility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This embodiment includes the following steps:

[0032] (1) Substrate pretreatment of self-assembled membrane

[0033] After surface treatment, the glass is immersed in a polyethyleneimine solution with a concentration of 0.01mg / mL to self-assemble to form the bottom layer;

[0034] (2) Preparation of plant polyphenol aqueous solution

[0035] Prepare 10ml of 10mM HEPES buffer solution, pH=4, add ascorbic acid to 5mM and tannic acid (TA) to 0.01mg / ml in turn, stir until completely dissolved, and prepare a tannic acid aqueous solution with a concentration of 0.01mg / mL;

[0036] (3) Preparation of protein aqueous solution

[0037] Configure 10ml of 10mM HEPES buffer solution, adjust the pH to 4, add 5mM to ascorbic acid and lysozyme to 0.01mg / ml in sequence, stir until completely dissolved, and prepare a lysozyme aqueous solution with a concentration of 0.01mg / mL;

[0038] (4) Preparation of self-assembled multilayer films

[0039] Immerse the substrate treated in step...

Embodiment 2

[0042] This embodiment includes the following steps:

[0043] (1) Substrate pretreatment of self-assembled membrane

[0044] After surface treatment, the glass is immersed in a polyethyleneimine solution with a concentration of 1 mg / mL to self-assemble to form the bottom layer;

[0045] (2) Preparation of plant polyphenol aqueous solution

[0046] Prepare 50mM HEPES buffer solution, pH=6, sequentially add sodium chloride to 0.15mol / l, ascorbic acid to 10mM and tannic acid (TA) to 1mg / mL, stir until completely dissolved, and prepare a single solution with a concentration of 1mg / mL. Nitric acid aqueous solution;

[0047] (3) Preparation of protein aqueous solution

[0048]Prepare 50mM HEPES buffer solution, pH=6, sequentially add sodium chloride to 0.15mol / l, ascorbic acid to 10mM and lysozyme to 1mg / mL, stir until completely dissolved, and prepare a lysozyme aqueous solution with a concentration of 1mg / mL;

[0049] (4) Preparation of self-assembled multilayer films

[0050...

Embodiment 3

[0052] This embodiment includes the following steps:

[0053] (1) Substrate pretreatment of self-assembled membrane

[0054] After surface treatment, the glass is immersed in a polyethyleneimine solution with a concentration of 10 mg / mL to self-assemble to form the bottom layer;

[0055] (2) Preparation of tannic acid aqueous solution

[0056] Prepare 50mM HEPES buffer solution, pH=8, sequentially add sodium chloride to 0.5mol / l, ascorbic acid to 50mM and tannic acid (TA) to 10mg / ml, stir until completely dissolved, and configure a single solution with a concentration of 10mg / mL Nitric acid aqueous solution;

[0057] (3) Preparation of lysozyme aqueous solution

[0058] Prepare 50mM HEPES buffer solution, pH=8, add sodium chloride to 0.5mol / l, ascorbic acid to 50mM and lysozyme to 10mg / ml in sequence, stir until completely dissolved, and prepare a lysozyme aqueous solution with a concentration of 10mg / mL;

[0059] (4) Preparation of self-assembled multilayer films

[0060...

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Abstract

The invention discloses a preparation method of a self-assembled lysozyme multilayer membrane with an antibacterial property and biocompatibility, and belongs to the technical field of preparation of biomedical materials. The preparation method comprises the following steps: preparing a self-assembled multilayer membrane substrate, preparing a plant polyphenol solution, preparing a lysozyme solution, preparing the self-assembled multilayer membrane and the like. With a strong hydrogen-bond interaction between molecules of plant polyphenol and lysozyme as a driving force in the deposition process, the main part of the self-assembled membrane does not use polycations, so that cell damage caused by multiple layers of polycations in the conventional self-assembled membrane can be effectively reduced; after the surface of a human implant is decorated with the self-assembled multilayer membrane, the antibacterial property and the biocompatibility of the implant can be significantly improved; and the self-assembled multilayer membrane is very wide in application prospect.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a method for preparing a self-assembled lysozyme multilayer film. Background technique [0002] Layer-by-layer self-assembly technology is an effective method for preparing thin films, which can be applied to the assembly of various materials, and is simple to operate and easy to control. Among them, the polyelectrolyte layer-by-layer self-assembly technology is to prepare a polyelectrolyte film on the surface of the substrate by soaking the substrate alternately in an anion-cation polyelectrolyte solution. The binding forces in the self-assembly process mainly include forces in the form of covalent bonds, coordination bonds, charge transfer, hydrogen bonds, and electrostatic attraction. Layer-by-layer self-assembly technology can realize the assembly on glass, titanium alloy, ceramic, quartz, single crystal silicon wafer, mica, gold, silver, alumina and polymer, and the shap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/34A61L27/28A61L27/54
CPCA61L27/28A61L27/34A61L27/54A61L2300/21A61L2300/254A61L2300/404A61L2300/608A61L2400/18
Inventor 昝兴杰杨硕硕
Owner WENZHOU INST OF BIOMATERIALS & ENG
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