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Method for enriching and purifying aloe polysaccharides in aloe

An aloe vera polysaccharide and enrichment technology, which is applied in respiratory system diseases, urinary system diseases, skin diseases and other directions, can solve the problems of low extraction purity and low extraction rate, and achieve the effects of no sample loss, high recovery rate and solvent saving.

Active Publication Date: 2011-06-22
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a preparation method for enriching and purifying aloe polysaccharides in aloe by high-speed countercurrent chromatography

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Preparation of aloe polysaccharides with a purity higher than 95% using ethyl acetate-n-butanol-water and chloroform-methanol-isopropanol-water two-solvent system: using a semi-preparative high-speed countercurrent chromatograph equipped with an NS-1007 pump, 20ml Injection valve, polytetrafluoroethylene column, the volume of the column is 240ml, 8823A-UV ultraviolet detector Yokogawa 3057 portable recorder. Before sample injection, fill the entire column with stationary phase, adjust the speed of the main engine to 800rpm, and pump the mobile phase into the column at a flow rate of 2.0ml / min. Detector UV spectra to receive target components. The preparation of the aloe polysaccharide is carried out in two steps: (1) Ethyl acetate-n-butanol-water with a volume ratio of 1:2:3 is miscible in a separatory funnel, shaken and then separated into layers. Get the upper layer solution (upper phase) as the stationary phase, and the lower layer solution (lower phase) as the mobi...

Embodiment 2

[0018] Aloe polysaccharides with a purity higher than 95% were prepared using ethyl acetate-isobutanol-acetone-water and chloroform-methanol-water two-solvent systems: semi-preparative high-speed countercurrent chromatography equipped with NS-1007 pump, 20ml sample injection Valve, Teflon column, column volume 240ml, 8823A-UV ultraviolet detector Yokogawa 3057 portable recorder. Before sample injection, fill the entire column with stationary phase, adjust the speed of the main engine to 800rpm, and pump the mobile phase into the column at a flow rate of 2.0ml / min. Detector UV spectra to receive target components. The preparation of aloe polysaccharide is carried out in two steps: (1) Ethyl acetate-isobutanol-acetone-water with a volume ratio of 5:40:1:60 is miscible in a separatory funnel, shaken and then separated into layers. Get the upper layer solution (upper phase) as the stationary phase, and the lower layer solution (lower phase) as the mobile phase. Dissolve 200 mg o...

Embodiment 3

[0020] Use n-butanol-water and dichloromethane-isopropanol-chloroform-methanol-water two-solvent system to prepare aloe polysaccharides with a purity higher than 95%: use a semi-preparative high-speed countercurrent chromatograph equipped with a NS-1007 pump, 20ml Injection valve, polytetrafluoroethylene column, the volume of the column is 240ml, 8823A-UV ultraviolet detector Yokogawa 3057 portable recorder. Before sample injection, fill the entire column with stationary phase, adjust the speed of the main engine to 800rpm, and pump the mobile phase into the column at a flow rate of 2.0ml / min. Detector UV spectra to receive target components. The preparation of aloe polysaccharide is carried out in two steps: (1) n-butanol-water with a volume ratio of 45:53 is miscible in a separatory funnel, shaken and then separated into layers. Get the upper layer solution (upper phase) as the stationary phase, and the lower layer solution (lower phase) as the mobile phase. Dissolve 150 m...

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Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for purifying aloe polysaccharides, particularly a preparation method for enriching and purifying aloe polysaccharides in aloe by a high-speed countercurrent chromatography separation process. The method is characterized by purifying natural plant aloe leaching liquor twice by a high-speed countercurrent chromatography separation process to obtain aloe polysaccharides of which the purity is higher than 95%. The method is suitable for preparing high-purity aloe polysaccharides from natural plants containing aloe polysaccharides and extracts of the natural plants. The method is suitable for preparing aloe polysaccharides by separating with various types of countercurrent chromatographs, and the aloe polysaccharides prepared by the high-speed countercurrent chromatography process can be continuously, efficiently and quickly separated by liquid-liquid partition chromatography; and thus, the method has the characteristics of great separation amount, no sample loss, high recovery rate, mild separation environment, solvent saving and the like.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry and relates to a method for purifying aloe polysaccharides, in particular to a preparation method for enriching and purifying aloe polysaccharides in aloe by using a high-speed countercurrent chromatographic separation method. Background technique: [0002] Aloe: Latin scientific name Aloe, also known as Luhui, Nehui, Elephant Gall, Nuhui, and Laowei; it is an evergreen and succulent herb of the Liliaceae family. The leaves are clustered, in a seat shape or on the top of the stem, the leaves are often lanceolate or short and wide, with sharp tooth-like spines on the edge. The inflorescences are umbel, raceme, spike, conical, etc., red, yellow or with red spots, with six petals and six pistils. The base of the perianth is multi-connected into a tube. The main ingredients contain aloe-emodin (Aloemodin), aloe-emodin (Aloin, Barbaloin), isomers of aloe-emodin (Isobarbaloin), high tower alo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P11/00A61P13/12A61P17/00A61P37/04
Inventor 姚德坤姚德利闫峰黄运生姚航郑君
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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