The invention discloses a
plant polyphenol extraction detection method based on synchronization of liquid
chromatography and
tandem mass spectrometry, and relates to the technical field of
analytical chemistry detection, and the method comprises the following steps: predicting a chromatographic
elution window through an
ultraviolet spectrum, calculating a dynamic synchronization index by fusing
mass spectrum
background noise data, and starting a synchronous extraction detection program according to the dynamic synchronization index. In the extraction process, the
mass spectrum scanning frequency and the
ion trap filling time are fed back and adjusted according to pressure and temperature data monitored in real time; and when a target chromatographic peak appears, triggering
mass spectrum monitoring channel switching and recording fragment
ion information. And finally, integrating the fragment
ion abundance ratio and a chromatographic peak shape parameter to carry out result
verification. According to the method, dynamic coordination of extraction, separation and detection links is realized, and the
signal-to-
noise ratio, the sensitivity and the method adaptability of
polyphenol compound detection in a complex sample are improved.