47results about How to "Separation environment relaxation" patented technology

The invention relates to a Chinese magnoliavine fruit monomer composition separation preparation method; ethanol extracts from Chinese magnoliavine fruit are extracted by petroleum ether, chromatography is carried out to the petroleum ether extracts by a silicagel column, and then the petroleum ether is eluted by petroleum ether-ethylacetate, and HPLC is used for monitoring, and then crude extract A and crude extract B are fraction-collected, and eluted; the volume ratio of eluant petroleum ether and ethylacetate is 3-5:1, the crude extract A and crude extract B are respectively applied to a high-speed counter-current chromatography for separation, so as to obtain SCHisanhenol, deoxyschizandrin, schizandrin B, schisandrin C, schizandrol A, schizandrol B and schisantherrin B monomers, and the purity is higher than 98 percent.

The invention relates to a method for preparing lactuca indica and lactucin, which is a method for using two separation processes to obtain lactuca indica and lactucin from cichorium glandulosum by a high-speed countercurrent chromatography. The method has the advantages of large fractional dose, small sample loss, high recovery rate, mild separation environment and solvent saving. A great amountof lactuca indica and lactucin crude products can be placed in a countercurrent chromatograph, the separation result achieves the high purity and good separation effect. The method is not only suitable for preparing the product with high purity from plant crude extract, but also suitable for purifying lactuca indica and lactucin crude extracts obtained by various approaches.

The invention belongs to the field of natural organic chemistry, and relates to a method for purifying aloe polysaccharides, particularly a preparation method for enriching and purifying aloe polysaccharides in aloe by a high-speed countercurrent chromatography separation process. The method is characterized by purifying natural plant aloe leaching liquor twice by a high-speed countercurrent chromatography separation process to obtain aloe polysaccharides of which the purity is higher than 95%. The method is suitable for preparing high-purity aloe polysaccharides from natural plants containing aloe polysaccharides and extracts of the natural plants. The method is suitable for preparing aloe polysaccharides by separating with various types of countercurrent chromatographs, and the aloe polysaccharides prepared by the high-speed countercurrent chromatography process can be continuously, efficiently and quickly separated by liquid-liquid partition chromatography; and thus, the method has the characteristics of great separation amount, no sample loss, high recovery rate, mild separation environment, solvent saving and the like.

A process for preparing high-purity rhodioloside from natural rhodiola rosea by high-speed counter-current chromatography includes extracting by high-speed counter-current chromatography and purifying twice to obtain high-purity product. Its advantages are high purity (98%), and high output rate, and saving solvent.

The invention relates to a method for preparing high-purity soybean saponin A and soybean saponin B by high-speed countercurrent chromatography, which comprises the following steps of: (1) selecting a solvent system which is provided with a fixed phase as an upper phase and a mobile phase as a lower phase and has four components with the volume ratio of 1-5 to 0.5-1 to 1-5 to 5-10; (2) using the fixed phase to fill a countercurrent chromatographic column, regulating the rotation speed of a host to be 600 rpm to 1000rpm and pumping the mobile phase into the column at the flow rate of 0.5 ml/min to 4ml/min; (3) after the whole system is in dynamic equilibrium, introducing samples through an introduction valve; and (4) receiving a target composition according to a UV atlas of a detector, compressing, freezing and crystallizing the fraction to obtain monomers of the soybean saponin A and the soybean saponin B. The soybean saponin A and the soybean saponin B prepared by the method can reach quite high purity and the preparation method has simple operation, high separation efficiency, great separation volume, high recovery rate and good repeatability.

The invention relates to a preparation method for a high-purity glabridin monomer, and relates to the preparation method for the high-purity glabridin monomer by separation from licorice. The preparation method mainly solves the problems of complicated preparation process, low separation efficiency, long separation time, and large consumption amount of a solvent. The method provided by the invention is characterized by comprising the following steps: preparation of a glabridin extract; concentration of glabridin by using a reversed-phase C18 column chromatography: successively eluting the extract with alcohols respectively with a concentration of 50%, 70% and 95%, respectively collecting eluents, and subjecting the collected eluent of the alcohol with the concentration of 70% to vacuum concentration until the eluent is dried so as to obtain crude glabridin; and separation of the above-mentioned crude glabridin with a high-speed countercurrent chromatographic technology: placing a separating solvent in a separatory funnel and carrying out complete shaking, then carrying out standing and layering, dissolving the above-mentioned crude glabridin in the separating solvent, and respectively measuring the concentrations of supernatant and subnatant solutions containing the glabridin with a highly-efficient liquid-phase chromatographic method. The preparation method for the high-purity glabridin monomer provided by the invention has short separation time and high recovery rate, is free of pollution, and saves the solvent.

The method for separating magnolol and honokial from Chinese medicinal material magnolia bark includes the following steps: (1) using organic solvent to repeatedly extract magnolia bark powder, combining extracts, concentrating, drying to obtain magnelia bark crude product; (2) pouring thue solvent white can be used in counter-current chromatographic separation into liquid separator so as to form mutually-insoluble two phase of solvent; (3) dissolving magnolia bark crude product in equivolume two-phase mixed solvent in the step (2); and (4), injecting one phase of two phase of solvent into separation column of counter-current chromatograph as immobile phase, injecting the solution obtained in step (3) into sample-injecting ring of coutner-current chromatograph, starting the counter-current chromatograph, and injecting another phase of two phase of solvent as mobile phase, making continuous separation and collection so as to respectively obtain purified magnolol and honokiol.

The method of separating oridonin from rabdosia is one continuous liquid-liquid distributing adverse current chromatographic method without needing of any solid support and thus caused adsorption, loss, property change and other troubles. The method has high peak form resolution, great separation amount, no sample loss, high recovering rate, mild separating circumstance and low solvent consumption, and may be used in separating great amount of coarse oridonin product to reach purity over 97 %. In addition, the present invention adopts relatively simple and cheap adverse current chromatograph and economic mixture solvent comprising two or more of normal alkane, halohydrocarbon, fatty alcohol, aliphatic ketone, aliphatic ester, ether and water.

A process for preparing liensinine, iso liensinine and methyl liensinine from the core of lotus seed by counterflow chromatography method features that its separating solvent is the mixture of two or more in n-alkanes, halohydrocarbon, emtrol, lipone, fatty ester, ether and water.

The present invention is high speed countercurrent chromatographic process for preparing high purity taxol compound. The process includes the following steps: 1. pre-treatment including heating and dissolving coarse taxol extract and fixed phase; 2. high speed countercurrent chromatographic separation through shaking the solvent system, standing to separate upper phase and lower phase, using the upper phase as the fixed phase, pumping the mobile phase into column, feeding sample through the valve and accepting target component based on the detected ultraviolet spectrum; and 3. post-treatment including collecting taxol component, decompression concentrating to obtain white solid, dissolving and recrystallizing, filtering and drying to obtain white needle crystal. The HPLC detection shows that the product has purity over 99 %, and the process has high yield and low cost.

The invention discloses a method for preparing high purity z-ligustilide and cnidilide A, which is characterized by adopting high-speed countercurrent chromatography and comprises the following steps: preparing the crude extract of rhizoma ligustici wallichii or angelica as a sample injection substance; preparing a solvent system forming stationary phase and mobile phase; filling the stationary phase into a countercurrent chromatography column; then rotating a host machine, pumping the mobile phase into the column, or simultaneously pumping the stationary phase and the mobile phase into the column and then rotating the host machine; and injecting samples from an injection valve, receiving target components according to a map of a detector and obtaining the z-ligustilide and cnidilide A through separation. The solvent system is formed by four components (A, B, C and D). The component A is n-alkane, the component B is fatty ether, the component C is fatty alcohol or fatty ketone and the component D is water. The z-ligustilide and cnidilide A with purity higher than 95% can be obtained by the method. The method is simple and convenient, easy to operate, high in recovery and easy to promote and use.

The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to a method for separating and purifying amentoflavone, in particular, a method for enriching and purifying the amentoflavone through a high speed countercurrent chromatography method. The method is characterized in that: two mutually-immiscible solvent systems are utilized to perform the separation and purification in a mode of high-speed planetary motion in a support pipe; the method is applicable for the countercurrent chromatographies with various types to separate the amentoflavone, and has advantages of no irreversible adsorption, no loss of sample, no pollution, high efficiency and speed, high recovery rate, mild separating environment, high product purity and large quantity separation, and is applicable for industrial production.

The invention relates to a preparation method for separating high-purity hispidulin and herba lycopi flavone from common sage herb by high-speed countercurrent chromatography. The preparation method comprises the following steps of: (1) preparing a proper solvent system and standing to laminate so as to obtain an upper phase and a lower phase; (2) selecting the upper phase as a stationary phase and the lower phase as a mobile phase, filling the fixed phase into a countercurrent chromatograph column, adjusting the rotating speed of a host to be 600 to 1,000 rpm, pumping the mobile phase into the column at the flow velocity of 0.5 to 5.0 ml/min, and performing sample introduction by a sample valve after the whole system establishes dynamic balance; and (3) receiving target components according to a detector ultraviolet spectra, concentrating and crystallizing to obtain the high-purity hispidulin and herba lycopi flavones. The method is applicable to various types of high-speed countercurrent chromatographs and preparation of calycosin with different content, and has the characteristics of large separation quantity, high recovery rate and simpleness and convenience of operation.