Preparation method for high-purity glabridin monomer

Abstract

The invention relates to a preparation method for a high-purity glabridin monomer, and relates to the preparation method for the high-purity glabridin monomer by separation from licorice. The preparation method mainly solves the problems of complicated preparation process, low separation efficiency, long separation time, and large consumption amount of a solvent. The method provided by the invention is characterized by comprising the following steps: preparation of a glabridin extract; concentration of glabridin by using a reversed-phase C18 column chromatography: successively eluting the extract with alcohols respectively with a concentration of 50%, 70% and 95%, respectively collecting eluents, and subjecting the collected eluent of the alcohol with the concentration of 70% to vacuum concentration until the eluent is dried so as to obtain crude glabridin; and separation of the above-mentioned crude glabridin with a high-speed countercurrent chromatographic technology: placing a separating solvent in a separatory funnel and carrying out complete shaking, then carrying out standing and layering, dissolving the above-mentioned crude glabridin in the separating solvent, and respectively measuring the concentrations of supernatant and subnatant solutions containing the glabridin with a highly-efficient liquid-phase chromatographic method. The preparation method for the high-purity glabridin monomer provided by the invention has short separation time and high recovery rate, is free of pollution, and saves the solvent.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of active components of traditional Chinese medicines, and in particular relates to a preparation method for separating and preparing high-purity glabridin monomer from licorice. Background technique [0002] Known licorice is a leguminous plant licorice ( Glycyrrhizauralensis Fisch.), Glycyrrhiza inflate ( Glycyrrhizainflate Bat.), or Glycyrrhiza glabra ( Glycyrrhiza glabra L) dry roots and rhizomes; licorice is sweet in taste, flat in nature, and non-toxic. It is a tonic traditional Chinese medicine, which has the functions of nourishing qi and invigorating the middle, relieving spasms and relieving pain, moistening the lungs and relieving cough, purging fire and detoxification, and reconciling various medicines; licorice glabra Glabridin is a unique isoflavone component of Glycyrrhizae glabra. Studies have shown that glabridin has strong antioxidant activity, in addition...

AI Technical Summary

Problems solved by technology

[0002] Known licorice is a leguminous plant licorice ( Glycyrrhizauralensis Fisch.), Glycyrrhiza inflate ( Glycyrrhizainflate Bat.), or Glycyrrhiza glabra ( Glycyrrhiza glabra L) dry roots and rhizomes; licorice is sweet in taste, flat in nature, and non-toxic. It is a tonic traditional Chinese medicine, which has the functions of nourishing qi and invigorating the middle, relieving spasms and relieving pain, moistening the lungs and relieving cough, purging fire and detoxification, and reconciling various medicines; licorice glabra Glabridin is a unique isoflavone component of Glycyrrhizae glabra. Studies have shown that glabridin has strong antioxidant activity, in addition to anti-atherosclerosis, lowering blood lipids, neuroprotection, enhancing memory, inhibiting melanin formation, Weight loss and other effects; Glabridin is the most expensive natural freckle-removing and whitening raw material in the world. Traditional methods such as extraction, silica gel column chromatography, recrystallization and liquid preparation are used to separate and purify glabridin monomer. The above-mentioned separation and purification process is complicated, the separation efficiency is low, the separation time is long, and the solvent consumption is large. Therefore, high-purity glabridin very expensive

Patent application numbers 200410079233.1 and 200880117804.2 are low-purity licorice glabridin products with a purity of only about 50%. Certain purity also can only reach more than 90%, can not satisfy the requirement of standard substance and reference substance; Patent application number is 200910311302.X adopts efficient preparation liquid phase method and can obtain the glabridin with purity greater than 98%, but solvent consumption is too much large, long separation time

Images