A kind of preparation method of high-purity corylides and dehydrocoylides

Abstract

The invention relates to a method for preparing high-purity costuslactone and dehydrocostus lactone through high-speed countercurrent chromatography, which comprises the following steps of: (1) preparing a proper solvent system, standing and demixing to obtain an upper phase and a lower phase; (2) selecting the upper phase as a fixed phase and the lower phase as a mobile phase, filling the fixed phase in a column of a countercurrent chromatograph, regulating the rotating speed of a main machine to be 600-1,000rpm, pumping the mobile phase into the column at a flow rate of 0.5-5.0ml / min, and sampling through a sampling valve after the whole system is dynamically balanced; and (3) receiving a target component according to an ultraviolet spectrum of a detector, concentrating, and crystallizing. The method is suitable for preparing the costuslactone and dehydrocostus lactone in various contents through various high-speed countercurrent chromatographs, and has the characteristics of large separation amount, high recovery rate, and simplicity and convenience of operation.

Description

technical field

[0001] The invention belongs to the field of preparation of lactones contained in cassia, in particular to a preparation method of high-purity cassialides and dehydrocassialides. Background technique

[0002] Muxiang is the dried root of Auckland ia lappaDecne, a plant of the family Asteraceae. It has the effects of promoting qi and relieving pain, invigorating the spleen and eliminating food. It is mainly used clinically for the treatment of ulcers, biliary colic, bronchial asthma and other diseases. . Woody incense mainly contains volatile oil, and its main components are woody hydrocarbon lactone and dehydrowoody lactone, both of which have smooth muscle relaxation and antispasmodic effects, are the main active ingredients of woody, and are also important indicators of woody quality control. Lactone components such as woody lactone and delactone volatile oil can resist the bronchoconstriction effect caused by histamine, acetylcholine and barium chloride, ...

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