Method for preparing lactuca indica and lactucin

A technology for the separation of radish and radish, which is applied in the field of preparation of the separation of raptor and raptor, and can solve the problems of poor retention of stationary phase, poor repeatability, and large damage to instruments, etc.

Active Publication Date: 2012-02-15
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
View PDF4 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported to separate radish and radish from chicory root by n-hexane-ethyl acetate-methanol-water (1.5:4:2.5:6) and ethyl acetate-methanol-water (20:2:20) systems. lettucein and other sesquiterpene components, but there are still some problems when using these solvent systems, firstly, the retention of the stationary phase is not good, and the repeatability is poor, and secondly, the ethyl acetate part of the root extract of Chicory is dissolved in these solvent systems Poor solubility, resulting in less sample loading and greater damage to the instrument, so it is very important to find a solvent system or solvent that improves solubility or helps solubility

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 500 g of chicory root and pulverize it, then use 70% ethanol to extract it by ultrasonic extraction, the liquid ratio is 1:5, the extraction temperature is 20°C, the extract is concentrated under reduced pressure until it has no alcohol smell, and the total extract is obtained;

[0019] Disperse the total extract in water, sequentially extract with petroleum ether, ethyl acetate, and n-butanol, and distill under reduced pressure to obtain the extracts of petroleum ether, ethyl acetate, and n-butanol respectively;

[0020] Take 1.5g of the extract from the part of ethyl acetate, dissolve it in ethyl acetate, separate it by chromatography on a 200-mesh silica gel column, and elute it with n-hexane-ethyl acetate solvent system at a volume ratio of 4-1:1-3. Collect 15-20ml, carry out follow-up inspection on the fractions by thin-layer chromatography (TLC), use petroleum ether: ethyl acetate 1:5 as the developing solvent, combine the same components, concentrate under re...

Embodiment 2

[0025] After taking 500 g of chicory root and pulverizing it, use 90% ethanol to extract with the help of ultrasound, the liquid ratio: 1:10, the extraction temperature is 30 ° C, the extract is concentrated under reduced pressure until it has no alcohol smell, and the total extract is obtained;

[0026] Disperse the total extract in water, sequentially extract with petroleum ether, ethyl acetate, and n-butanol, and distill under reduced pressure to obtain the extracts of petroleum ether, ethyl acetate, and n-butanol respectively;

[0027] Take 1.5g of the extract from the part of ethyl acetate, dissolve it in ethyl acetate, separate by chromatography on a 300-mesh silica gel column, and elute with n-hexane-ethyl acetate solvent system at a volume ratio of 4-1:1-3. Collect 18ml, carry out follow-up inspection of the fractions by thin-layer chromatography (TLC), use petroleum ether: ethyl acetate 1:5 as the developing solvent, combine the same components, concentrate under reduc...

Embodiment 3

[0032]After taking 500 g of chicory root and pulverizing it, extract it with anhydrous methanol by hot reflux extraction, the liquid ratio is 1:15, the temperature is 60°C, the extract is concentrated under reduced pressure until it has no alcohol smell, and the total extract is obtained;

[0033] Disperse the total extract in water, sequentially extract with petroleum ether, ethyl acetate, and n-butanol, and distill under reduced pressure to obtain the extracts of petroleum ether, ethyl acetate, and n-butanol respectively;

[0034] Take about 1.5 g of the extract from the ethyl acetate part, dissolve it in ethyl acetate, separate it by 200 mesh silica gel column chromatography, and elute it with n-hexane-ethyl acetate solvent system at a volume ratio of 4-1:1-3. Parts collected 20ml, and the fractions were tracked and checked by thin-layer chromatography (TLC), using sherwood oil: ethyl acetate 1: 5 as a developing solvent, combining the same components, and concentrating unde...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for preparing lactuca indica and lactucin, which is a method for using two separation processes to obtain lactuca indica and lactucin from cichorium glandulosum by a high-speed countercurrent chromatography. The method has the advantages of large fractional dose, small sample loss, high recovery rate, mild separation environment and solvent saving. A great amountof lactuca indica and lactucin crude products can be placed in a countercurrent chromatograph, the separation result achieves the high purity and good separation effect. The method is not only suitable for preparing the product with high purity from plant crude extract, but also suitable for purifying lactuca indica and lactucin crude extracts obtained by various approaches.

Description

technical field [0001] The invention relates to a preparation method for separating beletin and beletin from chicory by using high-speed countercurrent chromatography. Background technique [0002] Chicory is an annual herbaceous plant belonging to the genus Chicory of the Compositae family. It is a commonly used medicinal material of the Uyghur nationality. Its aerial part is used as medicine. Recently, it was found that the extract of chicory root has the effects of protecting the liver, lowering blood sugar, and lowering blood fat, and the seed extract has the effect of anti-hepatotoxicity. [0003] In recent decades, domestic and foreign scholars have continuously deepened the research on the phytochemistry of Cichorium. The research of domestic and foreign scholars mainly focuses on Cichorium (Cichoriumi intybus L.). Chemical research shows that: its main components include various terpenoids, flavonoids Cichorium glandulosum Boiss.et Huet. As a plant of the same genus...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/93
Inventor 信学雷张尧阿吉艾克拜尔·艾萨杨义巴杭
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap