66results about How to "Scale up production" patented technology

The invention discloses a synthetic method for semaglutide and belongs to the field of polypeptide synthesis. In the method, amino acids at 1-30 positions of a main sequence of the semaglutide is obtained by adopting solid phase synthesis, Val at 10 position and Ser at 11 position adopt Fmoc-Val10-Ser11 (Psi(Me, Me) pro)-OH; and an Lys side chain at 20 position adopts Fmoc-Lys(X)-OH. The method comprises the following steps of performing cracking precipitation to obtain a semaglutide crude peptide; and performing purification to obtain the semaglutide. In the method, by introducing the Fmoc-Val10-Ser11 (Psi(Me, Me) pro)-OH to solve the problem of difficult sequence synthesis of the semaglutide, difficult sequence synthesis becomes simple and easy; and meanwhile, the yield is greatly increased, the purity of the crude peptide is greatly improved, the production cost is greatly lowered, and industrial amplification production is facilitated.

The invention relates to a preparation method for hyaluronic acid functionalized nano fibers applied to capturing cancer cells in a targeted manner. The preparation method comprises the following steps: (1) by taking water as a solvent, preparing a PVA/PEI (Poly(Vinyl Alcohol)/Polyethylenimine) spinning solution and carrying out electrostatic spinning; drying and carrying out vacuum cross-linking treatment in a dyer containing a glutaraldehyde solution to obtain PVA/PEI nano fibers with good water stability; (2) adding activated hyaluronic acid into the PVA/PEI nano fibers with the good water stability; reacting on a shaking bed to obtain electrostatic spinning PVA/PEI nano fibers which are functionally modified by the hyaluronic acid; then adding triethylamine and acetic anhydride to carry out acetylation treatment on all residual amino groups on the surface of a PVA/PEI nano fiber film; and finally, washing and drying to obtain the product. The preparation method provided by the invention is low in raw material price and simple in preparation process; the prepared hyaluronic acid functionalized electrostatic spinning nano fibers have good appearance, structural stability, biocompatibility and blood compatibility and the application prospect is wide.

The invention discloses a preparation method of coal-based porous carbon based on potassium carbonate cyclic activation and a preparation method of biomass-based porous carbon, relates to a preparation method of the coal-based porous carbon and a preparation method of the biomass-based porous carbon, and aims to solve the problems that the pore structure of a porous activated carbon material obtained by a physical activation method is not developed, and the amount of an activating agent required by a chemical activation method is large. The preparation method comprises the following steps: sequentially crushing, grinding and screening raw materials, then carrying out ball milling mixing or liquid phase impregnation to mix a small amount of K2CO3 with a coal matrix, carrying out high-temperature activation on the obtained mixture in an atmosphere furnace, and cleaning and drying the activated product. According to the preparation method, the potassium carbonate can catalyze the activation reaction of an activation gas and the carbon matrix, so that the pore forming process is enhanced; in addition, the cyclic regeneration of the potassium-based component can be realized in the reaction process, so that coal or biomass-based porous carbon with developed pores can also be obtained under the addition of a small K2CO3 adding amount. The method is suitable for preparing the porous carbon.

This invention relates to a spray-pyrolysis process for preparing red fluorescent powder, whose chemical formula is (Li1-aNaa) (Eu1-b-c-dSmbYcGdd)(SiO2)1/6(W1-mMom)2O8, wherein, a is 0-0.8; b is 0-0.08; c is 0-0.08; d is 0-0.08; m is 0-1. The spray-pyrolysis process comprises: weighing soluble compounds of the cations according to the above chemical formula, preparing clear precursor solution, atomizing, reacting, and collecting the red fluorescent powder. The red fluorescent powder is approximately spherical, and has such advantages as high crystallinity, uniform particle size distribution, smooth particle surface, high luminescent efficiency, and high color purity. The spray-pyrolysis process has such advantages as simple process, easy operation, and high production efficiency, and is suitable for continuous production.

The invention relates to a preparation method of grafted starch. The preparation method comprises the following steps of firstly adopting an electrical Fenton method to pretreat a prepared starch milk suspension for breaking a starch crystalline region, so that molecular chains of starch molecules are stretched, and more reactive sites capable of being in graft polymerization are exposed; then dropwise adding grafting monomers in a pretreated system, continuously introducing oxygen and electrifiying, enabling generated hydroxyl radicals as an initiator to initiate a graft polymerization reaction of starch molecule free radials and the grafting monomers, and meanwhile avoiding the generation of homopolymers of the grating monomers as much as possible. Grafting treatment is carried out on starch by adopting the method, the grafting ratio can reach 69.2%, the grafting efficiency can reach 87.6%, and the content of the homopolymers is low; the grafted starch prepared by the method has a high water-absorbing quality or film-forming property; and besides, the method is simple in technological operation and post-treatment, mild in reaction condition, time-saving, efficient and little in environmental pollution, and is beneficial for expanding scale production.

The invention belongs to the technical field of fine chemical engineering, and particularly relates to a green preparation method for metal oxide nano material. A 'metal ion-thiocyanide' system is used as a precursor, and reacts with aqueous alkali at the room temperature to form a product. The prepared metal oxide nano material is packed in a sealed manner to form the product. Various metal oxidenano materials, various metal oxide composite nano materials and doped nano materials can be synthesized. The preparation method has universality, the process is simple, conditions are gentle, cleanproduction and transportation can be realized, and production scale can reach industrial scale. The prepared metal oxide nano material can serve as an advanced functional material and a chemical novelmaterial, and application value is obtained in extensive fields.

The invention provides a strategy for obtaining a novel high selenate tolerant strain by utilizing selenate to treat tea endophytic herbaspirillum spp WT00F and combining with a forced evolution method, and a method of the novel high selenate tolerant strain to produce red elemental selenium (Se<0>). Through selenate treatment and forced evolution, the tea endophytic herbaspirillum spp can be repeatedly transferred and cultured in a 200 mM selenate culture medium, so that the stable novel high selenate tolerant strain- tea Herbaspirillum sp.NTC00F can be finally formed, and selenate tolerancecan reach 800 mM. The tea Herbaspirillum sp.NTC00F is placed into a culture medium containing 400 mM of selenate to ferment; and the bacteria can reduce the selenate and efficiently produce the red nano elemental selenium, and the yield of the red nano elemental selenium can reach 1,000 [mu]g/ml. The prepared pure red nano elemental selenium can be used for pharmaceutical, agricultural and food processing, and is simple in technical operation, low in cost, green, environmentally-friendly and pollution-free.

The invention discloses a method for preparing 3-trifluoromethyl pyrrole boric acid, belonging to the technical field of organic chemical synthesis. The method is characterized by comprising the following steps: (I) with trifluoroacetic anhydride as a start raw material, generating 4-ethoxy-3-alkenyl trifluoroacetyl ketone through reaction between the trifluoroacetic anhydride and ethyl vinyl ether; (II) performing heating reflux of 4-ethoxy-3-alkenyl trifluoroacetyl ketone and hydrazine dihydrochloride in an ethanol solution to obtain 3-trifluoromethyl pyrrole; and (III) performing functional group conversion of 3-trifluoromethyl pyrrole to generate 1-methyl-3-trifluoromethyl-5-pyrrole boric acid, N-tert-butyl acyl-3-trifluoromethyl-5-pyrrole boric acid and 1-methyl-3-trifluoromethyl-4-pyrrole boric acid respectively. The method has the beneficial effects that the generated target compound has good purity and stable properties, the method is simple and easy to operate, and the preparation scale is easy to enlarge to realize industrial production.

The invention relates to a preparation method for a CT (computed tomography) contrast media based on the second-generation polyamide-amine dendrimer/gold nanoparticles. The preparation method comprises the following steps of: weighing the second-generation polyamide-amine dendrimers with tail ends which are amino groups in aqueous solution, adding chloroauric acid solution in a water-bath condition, and magnetically stirring and reacting for 3 hours; cooling at a room temperature and then performing acetylation treatment; dialyzing the solution after the reaction, performing freeze-drying treatment to obtain a final product; and evaluating the in-vivo and in-vitro CT contrast performances of the product. The product prepared by the preparation method disclosed by the invention is simple and environment-friendly in preparation process, good in stability and cytocompatibility, and capable of showing a good in-vivo CT contrast effect.

The invention belongs to the technical field of novel chemical materials and in particular relates to an insoluble inorganic salt micro-nano material as well as a preparation method and application thereof. The insoluble inorganic salt micro-nano material is synthesized by taking a metal ion- thiocyanide system as a precursor and taking the precursor to react with a water-soluble inorganic salt solution at room temperature, wherein when water-soluble inorganic salt is sodium carbonate and metal ions are calcium ions, the prepared inorganic salt micro-nano material is a calcium carbonate micro-nano material with a special shape; when the metal ions are indium ions, the prepared insoluble inorganic salt micro-nano material is an indium salt nano material. The calcium carbonate micro-nano material with the special shape can be used as a chemical material and a filling agent and is used for fields including rubber, plastic, coating, cosmetics and the like; the insoluble indium salt nano material can be used as a functional addition material and a doping material and is used for fields including functional materials, photoelectric functional devices and the like.

The invention discloses a method for synthesizing poly-substituted 2-aryl benzothiazoles by utilizing thiourea as a sulphur source. According to the method, thiourea reacts with benzyl chloride to generate an S-benzylisothiourea salt in situ. After that, through the aromatic nucleophilic substitution reaction of the obtained S-benzylisothiourea salt with 2-fluoronitrobenzene and then the one-step reduction (one-pot reaction) process, o-amino phenyl benzyl thioether as an intermediate product can be obtained. Finally, through the iron-catalyzed cross-dehydrogenative-coupling reaction of the o-amino phenyl benzyl thioether, a target product can be obtained. Compared with the traditional synthetic method, the method has the significant advantages of (1) short reaction step, wherein the target product can be synthesized through only three steps of simple reactions by utilizing simple chemical raw materials; (2) mild reaction condition, high atom economy, and relatively safe and cheap reaction reagents; (3) high reaction yield, good substrate tolerance and free of any dangerous or high-toxicity reagent. Therefore, the method might be applied to the large-scale production.

The invention discloses a method for preparing saturated potassium azacyclo-trifluoroborates, which comprises the following steps: 1. reacting pyrrolidine and piperidine used as initial raw materials and tert-butyl carbonic anhydride to generate N-tert-butyl carbonyl pyrrolidine and N-tert-butyl carbonyl piperidine, and performing heating reflux on the N-tert-butyl carbonyl piperazine used as an initial raw material and benzyl chloride to generate N-tert-butyl carbonyl-4-benzyl piperazine; and 2. by means of the orientation effect of the N-tert-butyl carbonyl, in a nitrogen protective atmosphere, reacting with sec-butyl lithium at low temperature, then reacting with trimethylborate to generate saturated o-boric acid, adding a saturated KHF2 solution without separating the boric acid, and performing post treatment to respectively generate a potassium N-tert-butyl carbonyl-2-pyrrolidine trifluoroborate, a potassium N-tert-butyl carbonyl-2-piperidine trifluoroborate and a potassium N-tert-butyl carbonyl-4-benzyl-2-piperazine trifluoroborate. These saturated potassium azacyclo-trifluoroborates prepared by the invention are all white solids, are stable at room temperature in an air atmosphere, belong to very important organic chemical reagents and medical intermediate compounds, and have wide application prospects. The method disclosed by the invention is simple to operate, utilizes the cheap and accessible initial raw materials, achieves high yield and favorable purity, is easy to implement scale-up production, and realizes commercialized production.

The invention provides a preparation method of a nucleic acid probe, which comprises the following steps: mixing and grinding a nucleic acid solid with an amino active group and an activated ester modified dye solid capable of reacting with the amino group, and carrying out one-step reaction to obtain the nucleic acid probe. The nucleic acid solid and the dye solid are mechanically mixed and ground into jelly, and no reaction solvent or other reaction reagent is added in the process. The preparation method of the nucleic acid probe is a solvent-free reaction, does not need a toxic organic solvent, and is more green and pollution-free; and the production process is greatly simplified, and production can be amplified by combining the ball mill.

The invention relates to harringtonines, and a preparation method and application thereof, belonging to the field of medicinal compounds and preparation methods thereof. The harringtonines have a structure as shown in a formula (I) which is described in the specification. The invention also provides the preparation method for the harringtonines. The preparation method comprises a step of reacting a compound as shown in a formula (11) which is described in the specification with a compound as shown in a formula (12) which is described in the specification in the presence of Lewis acid.

The invention relates to a key intermediate product compound A for preparing trabectedin and a method for preparing the compound A by using a compound D. According to the method disclosed by the invention, three-step reaction can be continuously operated, and individual separation and purification are not needed, and therefore, an operation is simple and convenient. The invention also relates to amethod for preparing a compound E through reduction of the compound A and application of the intermediate product. The method has the advantages of controllability, simpleness and convenience in operation and stable yield. The formula (1) is shown in the description.

The invention provides a renaturation and purification method of a recombinant human granulocyte colony stimulating factor. The renaturation and purification method comprises the steps of (1) preparing inclusion body dissolving liquid; (2) adding the inclusion body dissolving liquid to renaturation buffer liquid, adding cystine and cysteine, and performing renaturation; and (3) applying the obtained renaturation liquid to a weak cation chromatography column with a composite ligand for purification. The purification technology is simple and convenient to operate, short in consumption time, andlow in equipment requirement; the cost is saved, and the purification technology is suitable for amplified production scale; and besides, during renaturation, inclusion protein bodies are high in concentration and high in renaturation rate, and after chromatography purification, protein purity is high.

The invention discloses a snuffing type Chinese herbal medicine for preventing influenza. The Chinese herbal medicine is prepared from the following 13 raw herbal materials: cyrtomium rhizome, mint, honeysuckle stem, honeysuckle, radix isatidis, asarum, folia perillae acutae, fructus viticis, herba schizonepetae, lotus leaf, Chinese mosla Herb, eupatorium and borneol. By the Chinese herbal medicine which is not required to be orally taken, the purpose of preventing symptoms such as influenza, wind cold, wind-heat type common cold, initial febrile diseases and drunkenness. When the snuffing type Chinese herbal medicine is used, a user only needs to wear a snuffing type Chinese herbal medicine bag. The snuffing type Chinese herbal medicine has the advantages of good prevention effect, instant effect, effective treatment, no toxic or side effects, low using cost, convenience in use and the like.