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Method for degrading beta-1,4-glycosidic-bond-containing high-molecular-weight polysaccharides

A large molecular weight, glycosidic bond technology, applied in the electrolysis process, electrolysis components, electrolysis organic production and other directions, can solve the problems of unsuitable industrial expansion production, product structure and activity destructive, low degradation rate, etc., to improve cholesterol metabolism, The effect of clearing the intestines and improving glucose tolerance

Active Publication Date: 2016-12-07
北京颐方生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the cleavage of glycosidic bonds in the acid degradation method is random, and the molecular weight distribution of the product is wide, which is destructive to the structure and activity of the product.
The hydrogen peroxide degradation method requires the use of hydrogen peroxide, which is potentially dangerous during storage and transportation, and has a low degradation rate, so it is not suitable for industrialized expansion of production

Method used

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  • Method for degrading beta-1,4-glycosidic-bond-containing high-molecular-weight polysaccharides

Examples

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Embodiment 1

[0031] A kind of method that adopts electric Fenton method to degrade konjac polysaccharide: take by weighing 1200g konjac polysaccharide (molecular weight 500KDa), be dispersed in 1.2Kg dehydrated alcohol, join in 15L distilled water, make the sugar solution that concentration is 80mg / ml, pack into In an electrolytic cell with a volume of 24L, weigh 149.1g of sodium sulfate and dissolve it in the polysaccharide solution, adjust the pH to 3 with 1mol / L hydrochloric acid, use a 30cm×30cm wire mesh as the anode, and a 30cm×30cm porous graphite as the cathode, and the distance between the two electrodes is 7cm, connected to 13V DC, the current density is 10mA / cm 2, and use the aeration device to pass in purified air at a rate of 2.2L / min (or pass in oxygen at a rate of 0.7L / min, the aeration device contains two ventilation holes, and the two ventilation holes are blown into oxygen at the same time), per liter Thick konjac polysaccharide solution passes into the amount of air abou...

Embodiment 2

[0033] A kind of method that adopts electric Fenton method to degrade konjac polysaccharide: take by weighing 2700g konjac polysaccharide (molecular weight 500KDa), be dispersed in 2.2Kg dehydrated alcohol, join in 15L distilled water, make the sugar solution that concentration is 180mg / ml, pack In an electrolytic cell with a volume of 24L, weigh 127.8g of sodium sulfate and dissolve it in the polysaccharide solution, adjust the pH to 3 with 1mol / L hydrochloric acid, use a 30cm×30cm wire mesh as the anode, and a 30cm×30cm porous graphite as the cathode, and the distance between the two electrodes is 5cm, connected to 14V DC, the current density is 13mA / cm 2 , and use the aeration device to feed oxygen at a rate of 0.5L / min (the aeration device contains three air holes, and the three air holes are blown into oxygen at the same time), and the amount of air per liter of crude konjac polysaccharide solution is about 6.0L , the stirrer was stirred at a speed of 250rpm, and after el...

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Abstract

The invention relates to a method for degrading beta-1,4-glycosidic-bond-containing high-molecular-weight polysaccharides. The method comprises the following steps: introducing an 80-180 mg / mL beta-1,4-glycosidic-bond-containing high-molecular-weight polysaccharide solution into an electrolyzer provided with a cathode and an anode, regulating the solution to acidity, introducing 4.0-6.0L of oxygen to the cathode for every liter of the polysaccharide solution, and introducing a direct current while controlling the current density of the cathode at 10-13 mA / cm<2>; and under such conditions, carrying out degradation, and collecting the electrolyte. The method has favorable selectivity for beta-1,4-glycosidic-bond-containing polysaccharides, has the advantages of simple technical operation, time saving, high efficiency, mild reaction conditions and low environmental pollution, and is beneficial to scale-up production. More importantly, compared with the acid-process degradation and hydrogen peroxide degradation, the electro-Fenton process has higher degradation rate for polysaccharides, and the molecular weight range of the obtained low-molecular-weight polysaccharides is narrower.

Description

technical field [0001] The invention relates to the field of production of pharmaceutical raw materials, in particular to a method for degrading large molecular weight polysaccharides containing β-1,4-glucosidic bonds. Background technique [0002] Polysaccharide is formed by the condensation and dehydration of multiple monosaccharide molecules. It is a kind of carbohydrate with complex molecular structure and huge size. Active polysaccharide refers to a polysaccharide compound with special physiological activity, which has the function of regulating the circadian rhythm of the human body. Monosaccharides in polysaccharides are connected by glycosidic bonds, and glycosidic bonds include β-1,4-glycosidic bonds, α-1,3-glycosidic bonds, β-1,3-glycosidic bonds, etc. [0003] Studies have shown that the biological activity of polysaccharides is closely related to its molecular weight. The larger the molecular weight, the larger the molecular volume, and the poor solubility. lear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C25B3/00
CPCC08B37/009C25B3/00
Inventor 石清东王姣
Owner 北京颐方生物科技有限公司
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