106 results about "Carbohydrate Linkage" patented technology

To provide a technique capable of effectively recycling each recovered material as a single material by selectively decomposing, separating and recovering a fiber having a β - glycoside bond from a mixed fiber comprising the fiber having the β - glycoside bond such as cotton and other fibers such as polyester by a simple treatment in a short time under a non-pressurized condition.SOLUTION: A treatment method comprising selectively decomposing a fiber having a β - glycosidic bond from a mixed fiber containing the fiber having a β - glycosidic bond and a fiber composed of another material by a treatment using a deep eutectic solvent (DES) formed by mixing a hydrogen bond donor with a hydrogen bond acceptor at a molar ratio of 0.50 or more and 6.00 or less, and recovering the fiber composed of another material in a fibrous form.SELECTED DRAWING: None

The present disclosure relates to poly alpha-1,6-glucan ether compounds comprising poly alpha-1,6-glucan substituted with at least one positively charged organic group and having a degree of substitution of about 0.001 to about 3.0. The poly alpha-1,6-glucan comprises a backbone of glucose monomer units, wherein greater than or equal to 40% of the glucose monomer units are linked via alpha-1,6 glycosidic bonds, and optionally about 3% of the backbone glucose monomer units have branches via alpha-1,2 and / or alpha-1,3 glycosidic bonds. Compositions comprising poly alpha-1,6-glucan ether compounds can be used in various applications.

The invention discloses high-purity resistant dextrin and a preparation method thereof, and the preparation method comprises the following steps: (1) adding 4, 3-alpha-glucosyltransferase into a maltodextrin aqueous solution or a starch solution treated by starch debranching enzyme to react, and after the reaction is finished, deactivating the enzyme; (2) adding alpha-amylase into a reactant in the step (1) to react, and deactivating the enzyme after the reaction is finished; (3) adding alpha-glucosidase into the reactant in the step (2) for reaction, and deactivating the enzyme after the reaction is finished; (4) adding saccharifying enzyme into the reactant in the step (3) to react, and deactivating the enzyme after the reaction is finished; and (5) adding yeast powder into the reactant in the step (4) for fermentation, filtering by a filter membrane after fermentation, collecting membrane filtrate, and drying to obtain the high-purity resistant dextrin. The preparation process is simple and efficient, the product purity is high, the proportion of alpha-1, 3 glucosidic bonds in the prepared resistant dextrin is 40% or above, and the purity of the resistant dextrin is 80-90%.

The application discloses a method for controllably degrading glycosidic bonds of hyaluronic acid by programmed temperature rising. The method is used for high-molecular-weight hyaluronic acid aqueous solution, and a combination of an oxygen oxidant and an acidic additive is used to controllably cut and degrade glycosidic bonds in the macromolecule through a heating process of programmed temperature rising control system, so as to obtain oligomeric hyaluronic acid. The oligomeric hyaluronic acid prepared by the method has very good stability and no color change after long-time storage. The oligomeric hyaluronic acid prepared by the method can be applied to the fields of cosmetics, food, medicine and the like, and has very strong practical value.

The invention discloses a flavor modifier, atomized essence and an aerosol generating device, and relates to the technical field of essence. According to the technical scheme, the flavor modifier comprises an active agent, a compound containing a phenolic hydroxyl group and-O-beta-D-glucosidic bond or a compound containing an alcoholic hydroxyl group and-O-beta-D-glucosidic bond is selected as the active agent, and the flavor modifier can be weakly combined with a Piezo channel in a trigeminal nerve to directly modulate a trigeminal nerve mechanoreceptor, so that the flavor of the trigeminal nerve is improved. The activity of the Piezo channel is inhibited, so that the sensitivity of the Piezo channel to mechanical friction of aerosol particles is reduced, and the nasal cavity roughness is reduced. Meanwhile, the flavor modifier disclosed by the invention can also cooperate with olfactory signals transmitted by other components in the atomized essence to jointly improve the smoothness, the fineness and the overall moist feeling of the fragrance.

The present application relates to starch production technical field, especially disclose a kind of green preparation method for modified starch for replacing hydroxypropyl distarch phosphate.The green preparation method, cassava starch is used as raw material, specifically includes the following steps: (1) cassava starch is mixed with water uniformly, and starch suspension is prepared;(2) adding alpha-amylase to starch suspension for glycosidic bond enzymolysis, then passivate enzyme activity;(3) the enzymatic hydrolysate is used to hexose oxidase and oligomeric glucan is subjected to lactonization reaction, then passivate enzyme activity;(4) the final enzymatic hydrolysate is centrifuged, washed with water, the precipitate is freeze-dried, and enzyme modified cassava starch is obtained.The paste viscosity, freeze-thaw stability, shear resistance, anti-aging and other chemical properties of the enzyme modified cassava starch prepared by the present application are close to or better than hydroxypropyl distarch phosphate, and the preparation process is simple, short cycle, green and safe, low in production cost, simple in equipment, suitable for industrial production, and safe and pollution-free during production.

The invention discloses a compound microorganism fermented attenuated aroma-enhanced potato residue feed and a preparation method thereof, and belongs to the field of feed processing. The feed comprises potato residues, crop straw particles, corn flour, bran, soybean meal, urea, ammonium sulfate and a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis and lactobacillus plantarum. The preparation method comprises raw material pretreatment; preparing a microbial agent; performing mixed fermentation; and post-processing. The bacillus velezensis secretes glycosidase, and glucosidic bonds of the glycoalkaloid are subjected to enzymolysis; lactobacillus plantarum is metabolized to generate lactic acid to create an acidic environment to promote chemical degradation of glycoalkaloid, and the double bacteria cooperate to inhibit growth of mould, so that efficient attenuation is realized. The bacillus velezensis metabolizes to generate glucose, ethanol and free amino acid, and the lactobacillus plantarum metabolizes to generate ester flavor substances, so that the feed is endowed with strong fermentation aroma. Meanwhile, the pungent smell of volatile aldehyde substances is eliminated through double-bacterium synergism.

The invention provides emblic leafflower fruit polysaccharide with a weight losing effect and an efficient preparation method and application thereof, and belongs to the field of plant polysaccharide. The preparation method comprises the following steps: performing powdering, water extraction and concentration on a phyllanthus emblica raw material, adding an alcohol solvent for precipitation, and drying to obtain phyllanthus emblica crude polysaccharide; after the phyllanthus emblica crude polysaccharide is dissolved, impurities are removed through membrane filtration, protein substances are adsorbed through a reversed-phase chromatographic column, flow-through liquid and water leacheate are collected and combined, small-molecular-weight substances are removed through a membrane bag, intercepted liquid is collected and dried, and the phyllanthus emblica polysaccharide is obtained. The phyllanthus emblica polysaccharide has alpha-type and beta-type glycosidic bonds, and has linear polysaccharide chains and aggregates formed by a plurality of chains. The emblic leafflower fruit polysaccharide is used for preparing products for reducing fat and blood sugar, and can also be used for preparing medicines for preventing and treating abnormal glucose metabolism, abnormal lipid metabolism, related diseases, obesity and liver injury caused by obesity. The phyllanthus emblica polysaccharide extraction and purification preparation method is simple and efficient, and is convenient for industrial implementation.

This invention discloses a pullulanase mutant with enhanced catalytic activity against highly branched dextrin substrates, its construction method, and its applications, belonging to the fields of enzyme engineering and biotechnology. This invention obtains two mutants, D139A and D139E, by site-directed mutagenesis of the pullulanase derived from Klebsiella pneumoniae at position 139 aspartic acid residue. Compared with the wild-type enzyme, both mutants maintain high hydrolytic activity against pullulan polysaccharides while significantly improving hydrolytic activity against dextrin substrates with high α-1,6 glycosidic bond content. Specifically, the hydrolytic activity against dextrin substrates with 6% and 10% α-1,6 bond content is increased by up to 242% and 110%, respectively. The pullulanase mutants obtained in this invention exhibit excellent catalytic performance in the enzymatic debranching reaction of highly branched dextrin substrates, showing promising application prospects in starch deep processing and related biomanufacturing fields.

The invention belongs to the technical field of plant polysaccharide, and particularly discloses caulis spatholobi polysaccharide, a preparation method thereof and application of the caulis spatholobi polysaccharide in preparation of medicines for treating inflammation. Monosaccharide in the caulis spatholobi polysaccharide disclosed by the invention comprises mannose, glucuronic acid, galacturonic acid, glucose and arabinose in a molar ratio of (0.5-0.6): (0.3-0.5): (1-2): (1-2): (0.5-1.5). The caulis spatholobi polysaccharide provided by the invention is a novel uniform-structure polysaccharide which is separated from caulis spatholobi rattan for the first time, has a slightly acidic pH value, definite monosaccharide composition, definite molecular weight and clear glycosidic bond linking mode, has an anti-inflammatory effect, and is expected to be developed into a new anti-inflammatory drug. The preparation process is mild in condition, simple to operate and environment-friendly.

The invention discloses polysaccharide capable of improving symptoms of alcoholic liver diseases as well as a preparation method and application of the polysaccharide, and belongs to the technical field of biological medicines. The polysaccharide capable of improving the symptoms of the alcoholic liver disease is extracted and purified from yellow water, the molecular weight of the polysaccharide is 12 kDa to 16 kDa, the polysaccharide is formed by connecting glucose units through glucosidic bonds, and the main chain glucosidic bond connection mode is [-> 4-alpha-Glcp-1-> 4-alpha-Glcp-1-> 4-alpha-Glcp-1-> 4-alpha-Glcp-1->]. The polysaccharide provided by the invention shows a clear and powerful effect of improving the alcoholic liver disease on an animal model, is derived from a natural product, is small in expected toxic and side effects, high in safety, simple in preparation method, relatively low in cost and easy to realize large-scale production, and provides a new way for preventing and treating the alcoholic liver disease.

The present application belongs to the technical field of application of fungal polysaccharides, and particularly relates to a Boletus edulis fruiting body polysaccharide as well as a preparation method and application thereof. Polysaccharides in Boletus edulis fruiting bodies are extracted, and the chemical structure of BDP-F is characterized by ultraviolet-visible light, Fourier transform infrared spectroscopy, one-dimensional nuclear magnetic resonance and two-dimensional nuclear magnetic resonance. The research results show that BDP-F is mainly composed of mannose, glucose, galactose and fucose. Methylation and nuclear magnetic resonance analysis show that the glycosidic bond of BDP-F is (1→6)-alpha-d-galactose, the main chain is composed of ->6)-alpha-d-galp-(1→ residues, and the O-2 position is branched by alpha-L-Fucp-(1→ and alpha-D-Manp-(1→ residues. Through DPPH free radical, hydroxyl radical and superoxide anion radical scavenging experiments, it is shown that BDP-F has the ability to serve as a natural antioxidant, and BDP-F can also reduce alcohol-induced GES-1 cell damage through antioxidant and anti-inflammatory effects within the experimental concentration range.

The invention relates to a novel chemical cross-linking agent with mass spectrum fragmentable trehalose disaccharide as a skeleton structure and a preparation method thereof. The cross-linking agent disclosed by the invention has the following characteristics: 1) trehalose disaccharide is used as a skeleton structure, so that the cross-linking agent has excellent biocompatibility; 2) the trehalose skeleton has a pair of symmetrical mass spectrum fragmentable glucosidic bonds, so that a cross-linked peptide fragment can be simplified into a conventional peptide fragment modified by a cross-linking agent fragment; 3) the enrichment of the cross-linked peptide fragment can be realized by the trehalose skeleton under the condition of not adding an enrichment handle; and 4) active groups of the cross-linking agent comprise but not limited to a plurality of reactive groups such as succinamide ester, diaziridine, phenylsulfonyl fluoride, hydrazide group, amino group, hydroxylamine group and the like, and chemical cross-linking of a plurality of amino acids except lysine is realized. The trehalose cross-linking agent disclosed by the invention is applied to the field of proteomics, and provides technical support for realizing large-scale analysis of a protein complex in a complex sample, spatial structure analysis of protein and a protein-protein interaction network.

The invention relates to the technical field of carbohydrate informatics, in particular to a carbohydrate biomacromolecule property prediction method and system based on a graph neural network and chemical fingerprints. Comprising the following steps: modeling a carbohydrate chain sequence into an undirected graph, regarding monosaccharide and glucosidic bonds as nodes in the graph, introducing a virtual node for storing fingerprint features, extracting molecular fingerprint information from the sequence, constructing two different adjacent matrixes Afull and Aori with virtual node connection and without virtual node connection, and constructing two adjacent matrixes Afree and Aori; the dimension of the fingerprint features is reduced to be consistent with the node features, the features are replaced with molecular fingerprint features by positioning the positions of virtual nodes in the graph, the first three layers transmit Aori to enable a model to learn topological structure information of the graph, and the last layer transmits Afull to achieve fusion of the graph structure and the fingerprints. And pooling and splicing node features obtained after convolution of each layer to obtain final sugar chain representation for predicting different properties of sugar chains. According to the method, the graph structure and chemical fingerprint information of the sugar chain can be effectively combined to obtain more meaningful sugar chain representation.

This invention provides an extract of *Hedyotis diffusa*, its preparation method, and its uses. When the extract is measured under reversed-phase high-performance liquid chromatography, the peak area A of 6''-malonyl *Hedyotis diffusa* I is... M Peak area A with cypermethrin I B The ratio is less than 0.1, and the peak area A of free kaempferol is... K With A B The ratio shall not exceed 0.15. The preparation method includes: adjusting the pH of the aqueous extract of *Kaempferia galanga* to 11-13.5, and performing alkaline hydrolysis at 25-65°C for 20-60 minutes. This invention utilizes the difference in stability of malonyl bonds and glycosidic bonds under alkaline conditions to achieve the selective conversion of 6''-malonylkaempferol I to kaempferol I, increasing the content of kaempferol I while maintaining the integrity of the glycosidic bonds and inhibiting or controlling the formation of free kaempferol. The resulting extract can be used to prepare anti-inflammatory and antioxidant drugs or health foods.

This invention relates to the field of gene vector technology, and more particularly to an ionizable comb-like polymer, its preparation method, and its applications. This comb-like polymer is a block polymer, comprising at least one block unit. Each block unit includes a first block, a second block, and a third block linked by covalent bonds. It exhibits high molecular flexibility and readily binds to nucleic acid-like substances to form polymer / nucleic acid complexes. Due to the presence of easily degradable intracellular glycosidic bonds in its structure, the polymer demonstrates high transfection efficiency. Furthermore, cell experiments show that the polymer / nucleic acid complex exhibits low toxicity to cells and significantly improves cell survival compared to the positive control. Therefore, it holds promise for widespread application in cell transfection. The preparation method provided by this invention utilizes a redox amination tandem reaction to directly prepare the target product, eliminating the need for intermediate product purification processes. This simple process facilitates large-scale production.

The present application relates to the field of enzyme engineering, and in particular to a mutanase and application thereof. At present, mutan is mostly prepared by bacterial extraction, and there are problems such as low product yield and many impurities. The present application provides a mutanase, a MutI gene fragment of the mutanase is obtained by cloning a genome of Leuconostoc pseudomesenteroides G496, and a genetically engineered bacterium is constructed. The mutanase provided by the present application is subjected to enzymatic property analysis, and it is found that the mutanase has good stability under acidic conditions, and the optimal induction expression temperature and reaction temperature are close to room temperature, and the production energy consumption is low and the raw material conversion rate is high. The catalytic product of the mutant enzyme is linear mutan with a high polymerization degree, the molecular weight of the linear mutan is as high as 650 kDa, the linear mutan contains 90% of alpha-1,3 glycosidic bonds, and the structure is relatively dense, and the linear mutan has a good application prospect in the field of biological base materials.

The present application belongs to the field of computational chemistry and molecular simulation technology, and relates to a kind of aromatic eutectic solvent multiscale molecular simulation screening method and system based on multi-objective collaborative scoring model.Establish target molecule model library and eutectic solvent candidate component library;Quantum chemistry calculation is carried out on target molecules and eutectic solvent candidate system, and quantum chemistry descriptors are extracted;Molecular dynamics simulation system is constructed, and molecular dynamics descriptors such as aromatic space enrichment coefficient Kπ, glycosidic bond interaction index Ggly and lignin bond type affinity index Lβ are extracted;Multi-scale feature vectors are obtained by normalization processing, input into multi-objective collaborative scoring model, calculate and extract score SE, grading score SF, and judge and output active ingredient extraction priority, lignocellulose grading priority or segmented collaborative processing mode.The present application is suitable for eutectic solvent calculation screening and application mode discrimination when there is a target conflict between plant aromatic active ingredient extraction and lignocellulose grading treatment.

This invention discloses a fucose polymer, its preparation method, and its applications. Using natural fucoidan sulfate ester as a template, a fucose polymer with well-defined and homogeneous structures is prepared via an olefinic metathesis ring-opening polymerization reaction. The structure involves different glycosidic bond linkages, degrees of polymerization, sugar chain lengths, and sulfate sites. The amphiphilic fucose polymer self-assembles into spherical nanoparticles, exhibiting inhibitory activity against type I herpes simplex virus infection. The sulfated fucoidan polymer with a degree of polymerization of 20 shows the best antiviral effect. This invention achieves efficient and controllable synthesis of fucoidan oligosaccharide polymers and has the potential value as a novel anti-herpes simplex virus drug, which can be applied in the preparation of anti-herpes simplex virus drugs.

The application provides a high-stability gastrodin production process, which takes gastrodin tetraacetate as a starting material, and sequentially undergoes pentaacetylation reaction, centrifugation and drying to obtain a gastrodin pentaacetate intermediate; then gradient saponification refining is adopted, that is, preliminary hydrolysis is first carried out by using a low-concentration lye, and then deep saponification is carried out by using a high-concentration lye; after saponification, the product is subjected to centrifugation, drying and crushing to obtain a finished product; an ultrasonic-assisted crystallization step is added after saponification, and an inert gas atmosphere coating is carried out after crushing. The glycosidic bond breakage caused by strong alkali is effectively avoided through gradient saponification, and the key problems of low product purity and poor stability in the existing process are solved in combination with ultrasonic crystallization and inert gas protection. The obtained gastrodin product has the remarkable characteristics of high purity, few impurities and excellent stability, and has the following indexes: purity ≥ 99.1%, related substances ≤ 0.45%, and content decrease rate ≤ 0.8% after 18 months of accelerated test.

The application provides a synthesis method of chondroitin polysaccharide ABW90-1 and / or chondroitin polysaccharide ABW50-1, and relates to the technical field of polysaccharide preparation.The synthesis method provided by the application adopts a fructose 6-position picoloyl group as a guiding group, highly stereoselectively constructs beta-(2 to 6)-D-furan fructose and internal beta-(2 to 1)-D-furan fructose glycosidic bonds through a glycoside unit transfer strategy mediated by hydrogen bonds, and efficiently prepares chondroitin polysaccharide ABW90-1 and chondroitin polysaccharide ABW50-1.The synthesis method provided by the application has the advantages of simplicity, high efficiency, high target product yield and high purity, and has reference significance for the synthesis of other types of sucrose oligosaccharides.

The application provides a uridine monophosphate specific glycoside hydrolase and application thereof in biosynthetic synthesis of pseudouridine. Nmygdh The function of the gene is verified by in-vitro enzyme activity experiment Nmygdh The recombinant protein has broad-spectrum and high-efficiency catalytic activity, and can efficiently catalyze the hydrolysis of glycosidic bonds in various nucleotides, such as uridine monophosphate (UMP), guanosine monophosphate (GMP), adenosine monophosphate (AMP) and cytidine monophosphate (CMP), and the activity of uridine monophosphate is the strongest. The application uses the protein to efficiently hydrolyze uridine monophosphate to obtain two substrates of pseudouridine, i.e. uracil and 5'-phosphoribose, and realizes the atom-economical and efficient biosynthetic synthesis of pseudouridine by combining the cascade reaction of pseudouridine glycosidase EcPsuG and dephosphorylase EcYjjG.

The invention discloses a preparation method and application of aronia melanocarpa fruit homogeneous polysaccharide freeze-dried powder, and belongs to the field of biotechnology and functional food. By optimizing extraction, separation and purification processes, a homogeneous polysaccharide freeze-dried component is obtained from aronia melanocarpa fruits for the first time; the molecular mass, monosaccharide composition, glucosidic bond type and purity of the polysaccharide component are defined; in addition, it is further found from multiple aspects that the component has a good immunity enhancing effect and has important application potential in functional food and health care products for enhancing immunity.

This invention relates to a method for producing an aqueous polymer dispersion by free radical-initiated aqueous emulsion polymerization of a monomer composition comprising 50% to 99.9% by weight of at least one (meth)acrylic acid C1 to C2. 10 Alkyl esters and / or vinyl aromatic compounds (Class I), or at least one vinyl aromatic compound and conjugated aliphatic diene by weight of 50% to 99.9% (Class II), or vinyl acetate, vinyl propionate, vinyl tert-carbonate, long-chain fatty acid vinyl esters and / or ethylene by weight of 50% to 99.9% (Class III), these amounts being based on total monomers in each case, the method being carried out by polymerizing the composition in the presence of an oligosaccharide having 1.5 to 10 β-1,4-glycosidic bonds linking pyranose units; relating to the dispersion obtained accordingly; and relating to the use of the dispersion as a binder, adhesive, sizing agent for fibers, for the production of coatings or for the production of paper coating formulations.

The present invention relates to a method for detecting glycosidic bonds of plant polysaccharides. The method includes taking 100 μL of NaOH-DMSO suspension (1 mg / mL) of plant polysaccharides containing 20 or more kinds of substances such as galactan, araban, polygalacturonic acid, rhamnogalacturonan-I, rhamnogalacturonan-II, xyluronic acid, arabogalacturonic acid I and arabogalacturonic acid II, shaking at room temperature for 30 minutes, adding 40 μL of iodomethane and shaking for 1 hour. Such process is repeated three times to obtain methylated plant polysaccharides. The method further includes using an automatic detection equipment for methylation combined with PMP derivatization to analyze glycosidic bonds in polysaccharides. Not only the glycosidic bond information of neutral sugars can be analyzed in the subsequent LC-MS mass spectrometry analysis, but also the glycosidic bond information of acidic sugars can be resulted, and the analysis results are faster, more detailed and more reliable.

Disclosed herein are compositions comprising an aerogel or a hydrogel. The aerogel or hydrogel comprises a water insoluble alpha-glucan and a metal oxide. At least about 50% of the glycosidic bonds of the water-insoluble alpha-glucan are alpha-1, 3 bonds, and the metal oxide is selected from the group consisting of calcium oxide, magnesium oxide, titanium dioxide or any other suitable metal oxide. Also disclosed are methods of producing the aerogels and hydrogels, as well as methods of using these materials, as in absorption techniques. Other types of materials containing a water insoluble alpha-glucan are also disclosed.

This invention discloses a method for separating and purifying inulin, belonging to the field of natural plant powder separation and purification technology. The method includes: S1. Adding ethanol to an aqueous solution of natural inulin, performing a first alcohol precipitation at 2-5°C, separating to obtain a first supernatant and a precipitate; S2. Preparing the precipitate into an aqueous solution, preheating, subjecting to ultra-high pressure treatment, and freeze-drying to obtain medium- and long-chain inulin; S3. Freeze-drying the first supernatant to obtain a solid, redissolving the solid into an aqueous solution, adding ethanol, performing a second alcohol precipitation at -22 to -18°C, separating to obtain a second supernatant; S4. Freeze-drying the second supernatant, preparing an aqueous solution, ultrasonically treating at less than 40°C, and low-temperature drying to obtain short-chain inulin. The short-chain inulin of this invention employs graded alcohol precipitation assisted by temperature-controlled ultrasound to precisely break glycosidic bonds, resulting in a purity of 80.04%~92.55% and a yield of 42.90%~54.24%. The medium- and long-chain inulin employs graded alcohol precipitation combined with low-temperature preheating and ultra-high pressure coupling to improve its degree of polymerization and uniformity, resulting in a purity of 80.15%~87.84% and a yield of 31.53%~48.06%.

The invention relates to the technical field of feed additives, and provides an oligomeric atractylodes macrocephala polysaccharide BZ-3 as well as a preparation method and application thereof. The oligomeric atractylodes macrocephala polysaccharide BZ-3 is glucosan oligosaccharide composed of 14 monosaccharides, and specifically comprises the following components: 10 beta-furanfructose are connected through beta-1, 2 glucosidic bonds, then alpha-glucopyranose is connected through the beta-1, 2 glucosidic bonds, the tail end of the glucosan oligosaccharide is connected with 3 alpha-glucopyranose through alpha-1, 4 glucosidic bonds, and the molecular formula of the glucosan oligosaccharide is C72H144O72. According to the technical scheme, the problem that the application of the atractylodes macrocephala polysaccharide is limited due to the fact that the atractylodes macrocephala polysaccharide is insufficient in structural analysis and poor in quality controllability in the prior art is solved.

The invention belongs to the technical field of medicinal chemistry and pharmacology, and discloses a diphyllin amide derivative as well as a preparation method and application thereof. The diphyllin thioether derivative has a structure as shown in the following formula, wherein R represents one of phenyl, 3-fluorophenyl, 2-thienyl, 2-pyrrolyl and tert-butyl. The diphyllin amide derivative disclosed by the invention is a diphyllin amide derivative with a non-glycoside structure, does not contain structures such as glycosidic bonds and double bonds which are easy to hydrolyze in vivo, has the metabolic stability superior to that of glycoside compounds, has relatively strong tumor cell proliferation inhibition activity, and can be applied to preparation of medicines for treating cancers such as liver cancer, colorectal adenocarcinoma and lung cancer.

The invention relates to the technical field of enzyme engineering, in particular to recombinant sucrose phosphorylase or a functional fragment thereof, a preparation method and application thereof. The recombinant sucrose phosphorylase has at least one amino acid sequence as shown in SEQ ID NO: 1. The recombinant sucrose phosphorylase has the characteristics of high activity and high specificity, has specificity on catalysis of glucosidic bonds of polyphenol and glucoside, and can specifically catalyze 4'and 4 ''sites of polyphenol to form glucosidic bonds with glucose.