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Extraction process and application of peach gum polysaccharide

The technology of peach gum polysaccharide and peach gum is applied in the field of plant polysaccharide and the field of biomedicine, and can solve the problems of inability to meet the application requirements of medicines, content of peach gum polysaccharide, and difficulty in industrialized production, and achieve high yield, lower blood sugar, and loss of small effect

Active Publication Date: 2015-11-18
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the above defects or improvement needs of the prior art, the present invention provides a peach gum polysaccharide extraction process and its application, the purpose of which is to obtain a high yield and high purity by refining the extraction steps of peach gum polysaccharide The peach gum polysaccharide PGPSD, which solves the technical problems that peach gum polysaccharides in the past peach gum extracts contain a lot of impurities, have narrow application fields, are difficult to industrialize production, and cannot meet the requirements of pharmaceutical applications.

Method used

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  • Extraction process and application of peach gum polysaccharide
  • Extraction process and application of peach gum polysaccharide
  • Extraction process and application of peach gum polysaccharide

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preparation example Construction

[0035] According to one aspect of the present invention, a kind of preparation method of peach gum polysaccharide is provided, it is characterized in that, comprises the following steps:

[0036] (1) Remove impurities from natural peach gum, and swell it with water at a ratio of 0.5-1.5:100 to obtain a suspension, heat and dissolve the suspension, filter and centrifuge to obtain a supernatant; the temperature is preferably 90-100°C;

[0037] (2) Concentrating the supernatant, ethanol alcohol precipitation to obtain a precipitate, dissolving the precipitate in water, purifying, taking the supernatant, fading, concentrating and drying to obtain peach gum crude polysaccharide;

[0038] (3) The peach gum crude polysaccharide is configured into an aqueous solution with a mass volume ratio of 5%-12%, passes through a DEAE cellulose column, and is eluted with a water solvent eluent of 0.3mol / L-0.7mol / L. - Collect the polysaccharide solution within 16 hours;

[0039] (4) The collect...

Embodiment 1

[0054] (1) Remove impurities from the raw peach gum, swell at a material-to-liquid ratio of 1:100, and then dissolve at 100°C for 25-30 hours. Filter the obtained peach gum solution with four layers of gauze to remove impurities such as undissolved peach gum and bark for the first time; centrifuge the obtained supernatant at 3000rpm / min, and remove impurities such as dust for the second time.

[0055] (2) Concentrate the collected supernatant to about 1 / 4 of the original volume with a vacuum rotary evaporator, and carry out alcohol precipitation with ethanol overnight, and the obtained precipitate is crude polysaccharide. The crude polysaccharide was dissolved again in water, and the protein was removed by Sevage method. This process was repeated 3-4 times until the middle layer was clarified. Mix the resulting deproteoglycan solution and concentrate it with 30% H 2 o 2 For fading, stir evenly, leave it for several hours until the color is stable, and the fading is over. Co...

Embodiment 2

[0059] (1) Remove impurities from raw peach gum, swell at a solid-liquid ratio of 0.5:100, and then dissolve at 90°C for 25-30h. Filter the obtained peach gum solution with four layers of gauze to remove impurities such as undissolved peach gum and bark for the first time; centrifuge the obtained supernatant at 2000 rpm / min, and remove impurities such as dust for the second time.

[0060] (2) Concentrate the collected supernatant to about 1 / 4 of the original volume with a vacuum rotary evaporator, and carry out alcohol precipitation with ethanol overnight, and the obtained precipitate is crude polysaccharide. The crude polysaccharide was dissolved again in water, and the protein was removed by Sevage method. This process was repeated 3-4 times until the middle layer was clarified. Mix the resulting deproteoglycan solution and concentrate it with 30% H 2 o 2 For fading, stir evenly, leave it for several hours until the color is stable, and the fading is over. Concentrate to ...

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Abstract

The invention discloses an extraction process and application of peach gum polysaccharide (PGPSD). The process includes: water extraction and alcohol precipitation, protein removal by a Sevage reagent, dialysis, passing of a column with DEAE cellulose as the filler and other steps, thus obtaining finer polysaccharide (PGPSD). The PGPSD can be dissolved in water at room temperature, and is formed by connection of arabinose, mannose and galactose through a glycosidic bond. The peach gum polysaccharide (PGPSD) prepared by the process provided by the invention can reinforce the expression of insulin related transcription factors and glucose degradation key enzyme, has no toxicity to mice, and can significantly lower the blood glucose of diabetic mice.

Description

technical field [0001] The invention belongs to the fields of plant polysaccharides and biomedicine, and more specifically relates to an extraction process of peach gum polysaccharides and its application. Background technique [0002] Peach gum is the resin secreted from the main trunk, main branches, side branches and fruits of peach trees (Prunus persica (L) Batsch) or mountain peaches (Prunus davidiana) infected with gum disease. Peach gummosis is common in southern my country, seriously affecting orchard yield and fruit quality. More than 90% of the components of peach gum are polysaccharides, which are linked by arabinose, xylose, mannose, galactose and uronic acid with glycosidic bonds. The role of peach gum polysaccharide in medicine, printing and dyeing, storage and preservation and other industries has been gradually discovered and applied, and it has huge production value. [0003] But so far, the extraction of peach gum polysaccharides is relatively extensive, ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P3/10
Inventor 李国怀王雨亭马兆成
Owner HUAZHONG AGRI UNIV
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