Sea lettuce polysaccharide, preparation and use thereof
A reef film and polysaccharide technology, applied in the field of reef film polysaccharide and its preparation, achieves the effects of good anticoagulant activity and antithrombotic activity, no toxic side effects, and good market application prospects
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[0008] Below by embodiment the present invention will be further described.
[0009] Weigh the dried seaweed algae, add water 1 to 80 times the weight of the seaweed to soak and homogenate, stir and extract polysaccharides in a water bath at 40 to 100°C for 0.1 to 6 hours, centrifuge to discard impurities, take the clear liquid, and reduce the obtained clear liquid to Concentrate under pressure, then carry out dialysis or ultrafiltration to remove salt, concentrate the polysaccharide solution after removing the salt under reduced pressure, then perform enzymatic and / or Sevage method to remove the protein in the polysaccharide, and then concentrate the polysaccharide solution after removing the protein under reduced pressure, Adding 4 times the volume of ethanol with a concentration of 95% (weight percentage, the same below) to precipitate the polysaccharide, washing the resulting precipitate with absolute ethanol for 1 to 3 times, drying in an oven at 40 to 80°C for 0.01 to 8 h...
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