Microorganisms or/and biological enzyme extracting method for plant anthocyanin

An extraction method and anthocyanin technology, applied in the field of bioengineering, can solve the problems of large differences in individual biotransformation functions, low degree of anthocyanin degradation and absorption, degradation, and low metabolic rate, and achieve remarkable, reasonable and effective medical emergency treatment. Make full use of and avoid the effect of environmental pollution

Inactive Publication Date: 2010-06-30
安徽天紫花青素科技有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Anthocyanins are cationic anthocyanins in plants, that is, glycosides formed by the combination of flower color motifs and various monosaccharides through glycosidic bonds. This is a neutral substance with low biological activity. In the human body, it can only be degraded and desugared by human intestinal bacteria Cationic anthocyanins, i.e. flower color elements, can be absorbed and utilized by the human body, eliminate harmful free radicals in the human body, prevent and treat diseases, but this neutral anthocyanin degrades in the human body, has a very low metabolic rate, and has low bioavailability , so the body's absorption rate is also very low
[0004] In addition, the individual biotransformation function of people varies greatly, especially the intestinal degradation and absorption of anthocyanins is lower in middle-aged and elderly people and patients

Method used

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Experimental program
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Embodiment

[0050] When extracting, proceed as follows:

[0051] 1. Pretreatment of raw materials: fresh raw materials containing plant anthocyanins, such as grape skins, carrots, and indigo fruits, are classified → pulverized → soaked in soft water to absorb water → frozen to destroy plant tissue cell walls → heated and thawed → squeezed → filtered to make juice for later use ( 1) → add water to the residue → soak and stir → squeeze → filter to make juice for later use (2).

[0052] Two, the above-mentioned squeezed and filtered aqueous solution (1) and (2) are put together, and its water temperature is adjusted to the optimum range of following degrading microorganisms or / and biological enzymes.

[0053] 3. The above products are used in combination with lactic acid bacteria and bifidobacteria of human intestinal bacteria, etc., together with β-glucosidase, amygdalinase, invert sugar enzyme, maltosidase, β-glucosidase, and pectin lyase , the above-mentioned human intestinal bacteria an...

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Abstract

The invention relates to a Microorganisms or / and biological enzyme extracting method for plant anthocyanin, belonging to the technical field of biological engineering. The method comprises the following steps of: classifying plants containing anthocyanin for smashing, adsorbing water, freezing the plants, heating the plants for thawing, pressing the plants, filtering for making juice, cracking and degrading the anthocyanin by the microorganisms or / and the biological enzyme cracking anthocyanin glycosidic bond, utilizing an anion active agent for precipitation reaction, degrading the precipitate by the microorganisms, and carrying out precipitation separation and evaporation concentration. Cation anthocyanin with the real content of more than 95%, namely color primitive but not the anthocyanin can be obtained by the technology of the invention. The cation anthocyanin made by the invention does not need human intestinal microorganisms for degrading, and is directly adsorbed and utilizedby a human body or directly goes into human blood. The medical care value of the plant anthocyanin is much higher than that of the anthocyanin.

Description

Technical field: [0001] The invention relates to a method for extracting plant cationic anthocyanins in the technical field of bioengineering, in particular to a method for extracting plant anthocyanins by microorganisms or / and biological enzymes. Background technique: [0002] With the improvement of people's living standards and the pursuit of natural organic substances that are non-toxic and harmless to natural plant sources, which are beneficial to protecting people's health, preventing and curing diseases, and because artificially synthesized anthocyanins have a greater impact on the human body The toxic and side effects of cyanide have been banned by the World Health Organization. Therefore, efficient and non-toxic natural plant cationic anthocyanins and their extraction methods have become emerging frontier research topics in the 21st century. [0003] However, the existing separation and extraction technology only obtains anthocyanins containing anthocyanins, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P17/06C07D311/62C12R1/225C12R1/01C12R1/38C12R1/025C12R1/06C12R1/265C12R1/365C12R1/13C12R1/15C12R1/20C12R1/685
CPCY02P20/582
Inventor 耿兆翔
Owner 安徽天紫花青素科技有限公司
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