49results about How to "Good experimental basis" patented technology

The invention provides the construction for a farnesyl pyrophosphoric acid synthetase RNA (Ribonucleic Acid) interference recombinant lentivirus vector, which comprises the following steps of: sieving the most effective target sequence of an FDS (farnesyl diphosphate synthase) gene RNAi (RNA interference) in a tool cell 293T cell, synthesizing the double-stranded DNA of the most effective target sequence, connecting to a pGCSIL-GFP vector and successfully constructing the recombinant vector through enzyme cutting, sequencing and identification. Researches indicate that the constructed RNA interference vector LV-sh-FDS can downwards modulate the expression of an FDS mRNA (Messenger RNA) level in a neonatal rat cardiac myocyte, simultaneously can downwards modulate the expression of myocardial hypertrophy markers such as cell areas and marker genes beta-MHC (Myosin Heavy Chain) and BNP (Brain Natriuretic Peptide), additionally can effectively inhabit the activity of RhoA while downwards modulating the FDS, can be applied in preparing medicaments for treating myocardial hypertrophy diseases and also can be applied in preparing medicaments for cholesterol metabolic control.

The invention relates to the technical field of biomedicine, in particular to an antimicrobial polypeptide of which the amino acid sequence is shown as SEQ ID: NO: 1 or as SEQ ID: NO: 2. The antimicrobial polypeptide provided by the invention has the uses of resistance to drug-resistant pathogen infection, particularly resistance to drug-resistant super bacteria (super bacteria).

The invention relates to a preparation method for hyaluronic acid functionalized nano fibers applied to capturing cancer cells in a targeted manner. The preparation method comprises the following steps: (1) by taking water as a solvent, preparing a PVA/PEI (Poly(Vinyl Alcohol)/Polyethylenimine) spinning solution and carrying out electrostatic spinning; drying and carrying out vacuum cross-linking treatment in a dyer containing a glutaraldehyde solution to obtain PVA/PEI nano fibers with good water stability; (2) adding activated hyaluronic acid into the PVA/PEI nano fibers with the good water stability; reacting on a shaking bed to obtain electrostatic spinning PVA/PEI nano fibers which are functionally modified by the hyaluronic acid; then adding triethylamine and acetic anhydride to carry out acetylation treatment on all residual amino groups on the surface of a PVA/PEI nano fiber film; and finally, washing and drying to obtain the product. The preparation method provided by the invention is low in raw material price and simple in preparation process; the prepared hyaluronic acid functionalized electrostatic spinning nano fibers have good appearance, structural stability, biocompatibility and blood compatibility and the application prospect is wide.

The invention discloses a cable insulating layer specimen cutting device and cutting method. The device comprises a blade, a fixed shaft and a fixed base, wherein the fixed shaft comprises a first assembly section and a second assembly section which are connected with each other; the first assembly section is connected with a rotating shaft of a lathe, and the second assembly section is sleeved with a cable insulating layer specimen; the fixed base is arranged on the lathe, a blade groove matched with the blade is formed in the fixed base, the blade is fixedly arranged in the blade groove, and the edge of the blade is parallel to the axial direction of the fixed shaft. Along with rotation of the cable insulating layer specimen, the blade approaches the surface of the cable insulating layer specimen and cuts along the perimeter direction of the specimen to obtain a specimen slice, so that the inner, middle and outer layers of the cable insulating layer specimen can be definitely separated, and the thicknesses of the specimen slices are good in uniformity, so that an excellent experimental basis is provided to researches on the aging mechanism of insulating layer materials with different distances from cable cores.

The invention relates to an active gel electrophoresis method for lactalbumin. The method utilizes active acrylamide gel, mixes a lactalbumin sample with a buffer solution, and then directly performs electrophoretic analysis under the conditions as follows: an electrophoresis buffer solution is a Tris-glycine buffer solution with the pH of 8.3; the sample loading amount is that the ratio of lactalbumin to 2-times active protein sample buffer solution is 1:3-5, 7.5 mu L; the voltage is between 75 and 150 V; the time is between 25 and 75 minutes; and the temperature is between 2 and 4 DEG C. The active electrophoresis method has the advantages of small quantity of samples, high resolution, high sensitivity, good repeatability and capability of accurately analyzing the content of various types of lactalbumin and sensitively judging the processing techniques and quality situations of dairy products.

The invention discloses a perovskite solar cell taking Mo-SnO2 as an electron transport layer and a preparation method of the perovskite solar cell. The preparation method of the solar cell comprisesthe steps of (1) depositing a Mo-SnO2 electron transport layer on a clean ITO electrode; (2) depositing an NH4Cl/KCl interface modification layer on the Mo-SnO2 electron transport layer; (3) introducing a P123 copolymer into a (FAMA)CsPbIBr system; (4) depositing a (FAMA)CsPbIBr photosensitive layer on the NH4Cl/KCl interface modification layer; (5) depositing a Spiro-OMeTAD hole transport layer on the photosensitive layer; and (6) evaporating an Au counter electrode on the Spiro-OMeTAD hole transport layer. The average photoelectric conversion efficiency of the prepared solar cell reaches 22.83%, the highest photoelectric conversion efficiency exceeds 22.97%, and the solar cell has good illumination stability.

The invention provides a multifunctional measuring device for measuring influence of a particle system on a laser signal. The multifunctional measuring device comprises a particle suspension unit, a charge measurement unit and a laser signal measurement unit, wherein the particle suspension unit comprises a dustproof box and a particle storage box arranged in the dustproof box; a filtering net is arranged in the particle storage box, and fans are arranged on four walls of the particle storage box; laser through holes are formed in two sides of the dustproof box, respectively; laser lenses are mounted at the laser through holes; the charge measurement unit is used for measuring the electric charge of particles in the dustproof box; and the laser signal measurement unit is used for measuring change of laser power of the laser passing through the particle system. According to the measuring device disclosed by the invention, the influences of different particles on attenuation and depolarization of laser signals under conditions such as different humidity, different concentrations, different electric charges, and different particle size distributions can be accurately measured, a good experimental basis is provided for developing study of laser communication in sand and dust weather, and repeated measurement under the same condition can be realized.

The invention provides a method for constructing an adenovirus for efficient package and expression of melanoma differentiation-associated gene-7 (mda-7). The method adopts pDC316 belonging to adenovirus AdMax<TM> as a vector to construct and recombine an adenovirus expression vector to replace a most popular AdEasy system. Compared with the present most popular adenovirus AdEasy system, an adenovirus AdMax<TM> system has the characteristics of being simple and convenient to operate, high in recombination efficiency and high in yield (generally larger than 10<4>vp/cell) of obtained viruses. The method adopts the mda-7 which selectively induces apoptosis of tumor cells as a therapeutic gene to construct a replication-defective adenovirus vector Ad.mda-7.egfp through package of the mda-7 gene via the AdMax<TM> system, and testifies that the replication-defective adenovirus vector Ad.mda-7.egfp has the function of selectively inducing apoptosis of lung adenocarcinoma cell line A549, thereby laying a favorable experimental foundation for gene therapy of lung adenocarcinoma.

The invention belongs to the technical field of a non-viral gene vector and a medicine/photosencitizer vector, and particularly relates to a multifunctional nanometer vector and a preparation method of the multifunctional nanometer vector, wherein the multifunctional nanometer vector is built by using silicon oxide nanometer particles as substrates, functionalizing the silicon oxide nanometer particles through surface modification, introducing photosensitive groups with ultraviolet/near infrared light response and using an ATRP (Atom Transfer Radical Polymerization) method. The multifunctionalnanometer vector has higher transfection efficiency than international JINBIAO PEI in cells of HepG2, COS7, 293, C6, Hela and the like, and has photoresponse gene release performance. Under the photostimulation, the photosensitive groups on the surfaces of the silicon oxide nanometer particles perform photolysis, so that positive ions fall off, and the genes are released; and the gene transfection efficiency can be greatly improved. Various different medicine or photosencitizer molecules can be loaded into porous structures of mesoporous silicon oxide nanometer particles; and good experimentfoundation is laid for the development of the novel photoresponse nanometer vector. The method has the advantages that the use method is simple; and the commercialization potential is realized.

The invention relates to a preparation method for a CT (computed tomography) contrast media based on the second-generation polyamide-amine dendrimer/gold nanoparticles. The preparation method comprises the following steps of: weighing the second-generation polyamide-amine dendrimers with tail ends which are amino groups in aqueous solution, adding chloroauric acid solution in a water-bath condition, and magnetically stirring and reacting for 3 hours; cooling at a room temperature and then performing acetylation treatment; dialyzing the solution after the reaction, performing freeze-drying treatment to obtain a final product; and evaluating the in-vivo and in-vitro CT contrast performances of the product. The product prepared by the preparation method disclosed by the invention is simple and environment-friendly in preparation process, good in stability and cytocompatibility, and capable of showing a good in-vivo CT contrast effect.

The invention relates to a recombinant lentiviral vector aiming at hUHRF1 gene RNA (Ribonucleic Acid) interference and preparation thereof. The lentiviral vector aiming at ShRNA of the hUHRF1 gene is experimentally constructed; a synthetic DNA (Deoxyribonucleic Acid) segment aiming at the ShRNA is mediated through the lentiviral vector, and is in cotransfection 293 T cell culture with two vectors of pHelper 1.0 and pHelper 2.0; and after the recombinant lentiviral vector is obtained, a target cell is cotransfected, so that RNA interference aiming at the hUHRF1 gene is realized. The adopted inactive third-generation lentiviral vector (SIN) has the advantages of safety, reliability, capability of infecting nondividing cells, long-duration expression of integrating target genes into target cell gene groups, small immune reaction and the like, and is an ideal vector. According to the lentiviral vector, the interference effect on human breast cancer cells MCF-7 reaches 70-85 percent, so that the recombinant lentiviral vector lays a good experimental foundation for further research of relevant hUHRF1 genes, and can be widely used for in vivo gene treatment and gene function research.

The invention discloses a perovskite solar cell taking V-SnO2 as an electron transport layer and a preparation method of the perovskite solar cell. The preparation method of the perovskite solar cellcomprises the steps of (1) depositing a V-SnO2 electron transport layer on a clean ITO electrode; (2) depositing a KI interface modification layer on the V-SnO2 electron transport layer; (3) introducing a F127 copolymer into an (MAFA)CsPbIBr system; (4) depositing an (MAFA)CsPbIBr photosensitive layer on the KI interface modification layer; (5) depositing a Spiro-OMeTAD hole transport layer on thephotosensitive layer; and (6) evaporating an Au counter electrode on the Spiro-OMeTAD hole transport layer. The average photoelectric conversion efficiency of the solar cell prepared by the method reaches 22.72%, the highest photoelectric conversion efficiency exceeds 22.85% by optimizing a device preparation process, and the solar cell has good illumination stability.

The invention relates to an optical fiber electric field sensor based on the F-P interference principle. The sensor comprises an optical fiber electric field sensor body which is connected with an adjustable direct-current voltage source and an optical fiber sensor demodulator. The optical fiber sensor demodulator is connected with a computer. The manufacturing method of the optical fiber electricfield sensor comprises the following steps: inserting an optical fiber into a ceramic contact pin, and cutting a plastic plate into a circle with the same diameter as the piezoelectric ceramic piece;drilling a hole in the center of the plastic plate, and inserting the ceramic contact pin into the hole in the plastic plate; and arranging the piezoelectric ceramic piece and the plastic plate withthe ceramic contact pin in a stainless steel tube. The method for measuring by using the optical fiber electric field sensor comprises the following steps: introducing a direct-current voltage source;when the piezoelectric ceramic piece is subjected to an external electric field, enabling the piezoelectric ceramic piece to be subjected to telescopic deformation in the thickness direction; transmitting the data to the computer through a demodulator to obtain the variable quantity of the cavity length; and solving the electric field intensity according to the deformation quantity and an inversepiezoelectric coefficient. The sensor has the advantages of simple structure, small size, high precision and high response speed.

The invention relates to a preparation method of dendrimer stable bismuth sulfide nanoparticles. The preparation method comprises: (1) preparing a diluted acetic acid solution of hydroxyl-terminated fourth-generation polyamide-amine dendrimer and preparing a bismuth salt diluted acetic acid solution; and (2) mixing the diluted acetic acid solution of hydroxyl-terminated fourth-generation polyamide-amine dendrimer with the bismuth salt diluted acetic acid solution, introducing H2S gas, reacting under the protection of nitrogen gas for 1-2 hours, carrying out dialysis after the reaction is finished, and performing freeze-drying to obtain the dendrimer stable bismuth sulfide nanoparticles. The preparation method provided by the invention is simple to operate and has mild reaction conditions; and the prepared dendrimer stable bismuth sulfide nanoparticles have the advantages of narrow particle size distribution, good dispersion property and no agglomeration, can be potentially used in CT (computed tomography) imaging diagnosis, and has been successfully applied to the rabbit in-vivo imaging.

The invention discloses a method for rapidly carrying out variety analysis and content determination on anthocyanin in tomato fruits. Methanol/formic acid (9: 1, v/v) is used as a solvent; after an internal standard is added, anthocyanin in tomato fruits is subjected to liquid-liquid extraction, extraction is conducted for 12 h at a temperature of 4 DEG C, and supernate is taken through centrifugation; after membrane passing is conducted, mass spectrum information and spectral information of anthocyanin are collected at the same time, the anthocyanin in the tomato fruits is quantified, and structural analysis is conducted. According to the detection method, the pretreatment process is simple, the detection result is accurate, and tedious pretreatment purification steps are not needed. Themethod can be used for qualitatively and quantitatively analyzing anthocyanin in tomato fruits.

The invention provides an electric field sensor based on an electrostriction effect. The electric field sensor includes an electrostriction ceramic block shell, a groove body is arranged in the electrostriction ceramic block shell, a strain gauge is arranged in the groove body, the strain gauge comprises a strain gauge substrate, a grid structure is arranged on the strain gauge substrate, and thegrid structure is connected with one end of the connecting wire through an end point, and the other end of the connecting wire is connected with a bridge circuit, and the strain gauge is attached to the wall of the inner groove body of the electrostriction ceramic block shell through the adhesive. The invention aims to provide an electric field sensor which is simple in structure, small in size, low in power consumption, and can better realize the electric field intensity measurement.

The invention relates to a CT/MR/near infrared optical three-mode targeted contrast agent based on the lung non-cellule cancer as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, modifying the amino terminal of a fifth-generation polyamide-amine dendrimer with DOTA-NHS; secondly, connecting folic acid to the dendrimer by polyethylene glycol; thirdly, further modifying with Cy5.5; fourthly, preparing gold nanoparticles by using the modified dendrimer as a template through sodium borohydride reduction, and chelating gadolinium ions to the dendrimer; fifthly, carrying out complete acetylization on amino remained on the surface of the dendrimer; and finally, dialyzing the reacted solution, lyophilizing to obtain a final product, and evaluating in-vivo and in-vitro CT/MR/near infrared optical three-mode contrast performance of the product. The method is simple and feasible, has excellent in-vivo and in-vitro lung non-cellule cancer cell target effect, and has a potential application prospect of targeted CT/MR/near infrared optical contrast.

The invention belongs to the technical field of traditional Chinese medicine anticancer treatment and particularly relates to a traditional Chinese medicine extract composition, a melanoma inhibitingdrug, a preparation method and application. The traditional Chinese medicine extract composition comprises a ligustrum lucidum alcohol extract and a herba ecliptae alcohol extract which are in a crudedrug ratio of (0.5-1.5): 1. According to the traditional Chinese medicine extract composition, the action of inhibiting activity of melanoma cells is exerted through inhibiting cell cycle G2/M-stagetime phase, cell proliferation and cell cloning, so that the apoptosis of the melanoma cells is promoted, a good experiment foundation is settled for traditional Chinese medicine treatment on tumors such as malignant melanoma, and a new view is developed. The traditional Chinese medicine extract composition can be used for preparing drugs for inhibiting melanoma and is applied to treatment or deepresearches on the melanoma.