49results about How to "Good experimental basis" patented technology

The invention provides the construction for a farnesyl pyrophosphoric acid synthetase RNA (Ribonucleic Acid) interference recombinant lentivirus vector, which comprises the following steps of: sieving the most effective target sequence of an FDS (farnesyl diphosphate synthase) gene RNAi (RNA interference) in a tool cell 293T cell, synthesizing the double-stranded DNA of the most effective target sequence, connecting to a pGCSIL-GFP vector and successfully constructing the recombinant vector through enzyme cutting, sequencing and identification. Researches indicate that the constructed RNA interference vector LV-sh-FDS can downwards modulate the expression of an FDS mRNA (Messenger RNA) level in a neonatal rat cardiac myocyte, simultaneously can downwards modulate the expression of myocardial hypertrophy markers such as cell areas and marker genes beta-MHC (Myosin Heavy Chain) and BNP (Brain Natriuretic Peptide), additionally can effectively inhabit the activity of RhoA while downwards modulating the FDS, can be applied in preparing medicaments for treating myocardial hypertrophy diseases and also can be applied in preparing medicaments for cholesterol metabolic control.

The invention relates to a preparation method of a hepatocarcinoma target CT (Computed Tomography) contrast medium based on Au DENPs-LA (Dendrimer-entrapped Gold Nanoparticles-Lactobionic acid). The method comprises the following steps of: modifying a fifth generation polyamide-amine dendrimer of an amino terminal with fluorescein, connecting lactobionic acid with the dendrimer through polyethylene glycol, taking the modified dendrimer as a template, preparing gold nanoparticles by reduction of sodium borohydride, performing acetylation treatment on left amino on the surface of the dendrimer wholly, dialyzing a reacted solution, performing freeze drying treatment, obtaining a finished hepatocarcinoma target CT contrast medium, and evaluating the external CT performance and internal CT performance of the contrast medium. The method is simple and easy to implement; prepared Au DENPs-LA has good stability, aqueous solution dispersity and biocompatibility, shows a good external hepatocarcinoma cell target effect, has a specific target tumour CT effect, and has a potential target CT application prospect.

The invention relates to the technical field of biomedicine, in particular to an antimicrobial polypeptide of which the amino acid sequence is shown as SEQ ID: NO: 1 or as SEQ ID: NO: 2. The antimicrobial polypeptide provided by the invention has the uses of resistance to drug-resistant pathogen infection, particularly resistance to drug-resistant super bacteria (super bacteria).

The invention relates to a preparation method for hyaluronic acid functionalized nano fibers applied to capturing cancer cells in a targeted manner. The preparation method comprises the following steps: (1) by taking water as a solvent, preparing a PVA / PEI (Poly(Vinyl Alcohol) / Polyethylenimine) spinning solution and carrying out electrostatic spinning; drying and carrying out vacuum cross-linking treatment in a dyer containing a glutaraldehyde solution to obtain PVA / PEI nano fibers with good water stability; (2) adding activated hyaluronic acid into the PVA / PEI nano fibers with the good water stability; reacting on a shaking bed to obtain electrostatic spinning PVA / PEI nano fibers which are functionally modified by the hyaluronic acid; then adding triethylamine and acetic anhydride to carry out acetylation treatment on all residual amino groups on the surface of a PVA / PEI nano fiber film; and finally, washing and drying to obtain the product. The preparation method provided by the invention is low in raw material price and simple in preparation process; the prepared hyaluronic acid functionalized electrostatic spinning nano fibers have good appearance, structural stability, biocompatibility and blood compatibility and the application prospect is wide.

The invention discloses a cable insulating layer specimen cutting device and cutting method. The device comprises a blade, a fixed shaft and a fixed base, wherein the fixed shaft comprises a first assembly section and a second assembly section which are connected with each other; the first assembly section is connected with a rotating shaft of a lathe, and the second assembly section is sleeved with a cable insulating layer specimen; the fixed base is arranged on the lathe, a blade groove matched with the blade is formed in the fixed base, the blade is fixedly arranged in the blade groove, and the edge of the blade is parallel to the axial direction of the fixed shaft. Along with rotation of the cable insulating layer specimen, the blade approaches the surface of the cable insulating layer specimen and cuts along the perimeter direction of the specimen to obtain a specimen slice, so that the inner, middle and outer layers of the cable insulating layer specimen can be definitely separated, and the thicknesses of the specimen slices are good in uniformity, so that an excellent experimental basis is provided to researches on the aging mechanism of insulating layer materials with different distances from cable cores.

The invention relates to an in-situ preparation method of dendrimer / gold-silver alloy nanoparticle with a computed tomography (CT) radiography function, comprising the following steps: (1) weighing the fifth generation of polyamide-amine dendrimer of which the tail end is an amino, and preparing 9.7-14.9mg / mL of solution; and (2) in the above solution, adding silver nitrate solution, then addingchloroauric acid solution, adding sodium borohydride solution after stirring, and continuing stirring and reacting for 2-3 hours, dialyzing the reacted solution, freezing and drying to obtain an acetylated pre-product. The dendrimer / gold-silver alloy nanoparticle prepared by the method of the invention has narrow size distribution, good dispersibility and no agglomeration phenomenon. The X-ray attenuation intensity test shows that the X-ray attenuation intensity of alloy nanoparticle is in rising tendency and gradually shows the CT imaging property superior to that of the traditional contrast agent Iohexol.

The invention relates to an active gel electrophoresis method for lactalbumin. The method utilizes active acrylamide gel, mixes a lactalbumin sample with a buffer solution, and then directly performs electrophoretic analysis under the conditions as follows: an electrophoresis buffer solution is a Tris-glycine buffer solution with the pH of 8.3; the sample loading amount is that the ratio of lactalbumin to 2-times active protein sample buffer solution is 1:3-5, 7.5 mu L; the voltage is between 75 and 150 V; the time is between 25 and 75 minutes; and the temperature is between 2 and 4 DEG C. The active electrophoresis method has the advantages of small quantity of samples, high resolution, high sensitivity, good repeatability and capability of accurately analyzing the content of various types of lactalbumin and sensitively judging the processing techniques and quality situations of dairy products.

The invention discloses a perovskite solar cell taking Mo-SnO2 as an electron transport layer and a preparation method of the perovskite solar cell. The preparation method of the solar cell comprisesthe steps of (1) depositing a Mo-SnO2 electron transport layer on a clean ITO electrode; (2) depositing an NH4Cl / KCl interface modification layer on the Mo-SnO2 electron transport layer; (3) introducing a P123 copolymer into a (FAMA)CsPbIBr system; (4) depositing a (FAMA)CsPbIBr photosensitive layer on the NH4Cl / KCl interface modification layer; (5) depositing a Spiro-OMeTAD hole transport layer on the photosensitive layer; and (6) evaporating an Au counter electrode on the Spiro-OMeTAD hole transport layer. The average photoelectric conversion efficiency of the prepared solar cell reaches 22.83%, the highest photoelectric conversion efficiency exceeds 22.97%, and the solar cell has good illumination stability.

The invention provides a separating and purifying process of isochrysis galbana exopolysaccharide; and the process comprises the following steps of: preparing crude isochrysis galbana exopolysaccharide by using isochrysis galbana culturing solution as a raw material; separating the crude isochrysis galbana exopolysaccharide ECPS by ion exchange chromatography, so as to obtain neutral polysaccharide and acidic polysaccharide components; loading the neutral polysaccharide and acidic polysaccharide components on a gel column for chromatography, so as to obtain eight components, wherein except for an ECPSII-A component, the quantities of the rest polysaccharide components are small; purifying the ECPSII-A component by using the gel column chromatography, so as to obtain a single polysaccharide component; and depositing, freezing and drying the single polysaccharide component by ethanol so as to prepare pure exopolysaccharide ECPSIII. According to the invention, the separating and purifying process of the isochrysis galbana exopolysaccharide is established; and the process is simple and reasonable and has the advantages of stable reproducibility and good operability. A good experiment basis is laid for follow-up study on biological activity of the polysaccharide; moreover, the microalgae polysaccharide can be applied to the medicine and medical field like other polysaccharide substances.

The invention provides a multifunctional measuring device for measuring influence of a particle system on a laser signal. The multifunctional measuring device comprises a particle suspension unit, a charge measurement unit and a laser signal measurement unit, wherein the particle suspension unit comprises a dustproof box and a particle storage box arranged in the dustproof box; a filtering net is arranged in the particle storage box, and fans are arranged on four walls of the particle storage box; laser through holes are formed in two sides of the dustproof box, respectively; laser lenses are mounted at the laser through holes; the charge measurement unit is used for measuring the electric charge of particles in the dustproof box; and the laser signal measurement unit is used for measuring change of laser power of the laser passing through the particle system. According to the measuring device disclosed by the invention, the influences of different particles on attenuation and depolarization of laser signals under conditions such as different humidity, different concentrations, different electric charges, and different particle size distributions can be accurately measured, a good experimental basis is provided for developing study of laser communication in sand and dust weather, and repeated measurement under the same condition can be realized.

The invention provides a method for constructing an adenovirus for efficient package and expression of melanoma differentiation-associated gene-7 (mda-7). The method adopts pDC316 belonging to adenovirus AdMax<TM> as a vector to construct and recombine an adenovirus expression vector to replace a most popular AdEasy system. Compared with the present most popular adenovirus AdEasy system, an adenovirus AdMax<TM> system has the characteristics of being simple and convenient to operate, high in recombination efficiency and high in yield (generally larger than 10<4>vp / cell) of obtained viruses. The method adopts the mda-7 which selectively induces apoptosis of tumor cells as a therapeutic gene to construct a replication-defective adenovirus vector Ad.mda-7.egfp through package of the mda-7 gene via the AdMax<TM> system, and testifies that the replication-defective adenovirus vector Ad.mda-7.egfp has the function of selectively inducing apoptosis of lung adenocarcinoma cell line A549, thereby laying a favorable experimental foundation for gene therapy of lung adenocarcinoma.

The invention belongs to the technical field of a non-viral gene vector and a medicine / photosencitizer vector, and particularly relates to a multifunctional nanometer vector and a preparation method of the multifunctional nanometer vector, wherein the multifunctional nanometer vector is built by using silicon oxide nanometer particles as substrates, functionalizing the silicon oxide nanometer particles through surface modification, introducing photosensitive groups with ultraviolet / near infrared light response and using an ATRP (Atom Transfer Radical Polymerization) method. The multifunctionalnanometer vector has higher transfection efficiency than international JINBIAO PEI in cells of HepG2, COS7, 293, C6, Hela and the like, and has photoresponse gene release performance. Under the photostimulation, the photosensitive groups on the surfaces of the silicon oxide nanometer particles perform photolysis, so that positive ions fall off, and the genes are released; and the gene transfection efficiency can be greatly improved. Various different medicine or photosencitizer molecules can be loaded into porous structures of mesoporous silicon oxide nanometer particles; and good experimentfoundation is laid for the development of the novel photoresponse nanometer vector. The method has the advantages that the use method is simple; and the commercialization potential is realized.

The invention relates to a preparation method for a CT (computed tomography) contrast media based on the second-generation polyamide-amine dendrimer / gold nanoparticles. The preparation method comprises the following steps of: weighing the second-generation polyamide-amine dendrimers with tail ends which are amino groups in aqueous solution, adding chloroauric acid solution in a water-bath condition, and magnetically stirring and reacting for 3 hours; cooling at a room temperature and then performing acetylation treatment; dialyzing the solution after the reaction, performing freeze-drying treatment to obtain a final product; and evaluating the in-vivo and in-vitro CT contrast performances of the product. The product prepared by the preparation method disclosed by the invention is simple and environment-friendly in preparation process, good in stability and cytocompatibility, and capable of showing a good in-vivo CT contrast effect.

The invention relates to a recombinant lentiviral vector aiming at hUHRF1 gene RNA (Ribonucleic Acid) interference and preparation thereof. The lentiviral vector aiming at ShRNA of the hUHRF1 gene is experimentally constructed; a synthetic DNA (Deoxyribonucleic Acid) segment aiming at the ShRNA is mediated through the lentiviral vector, and is in cotransfection 293 T cell culture with two vectors of pHelper 1.0 and pHelper 2.0; and after the recombinant lentiviral vector is obtained, a target cell is cotransfected, so that RNA interference aiming at the hUHRF1 gene is realized. The adopted inactive third-generation lentiviral vector (SIN) has the advantages of safety, reliability, capability of infecting nondividing cells, long-duration expression of integrating target genes into target cell gene groups, small immune reaction and the like, and is an ideal vector. According to the lentiviral vector, the interference effect on human breast cancer cells MCF-7 reaches 70-85 percent, so that the recombinant lentiviral vector lays a good experimental foundation for further research of relevant hUHRF1 genes, and can be widely used for in vivo gene treatment and gene function research.

The invention discloses a perovskite solar cell taking V-SnO2 as an electron transport layer and a preparation method of the perovskite solar cell. The preparation method of the perovskite solar cellcomprises the steps of (1) depositing a V-SnO2 electron transport layer on a clean ITO electrode; (2) depositing a KI interface modification layer on the V-SnO2 electron transport layer; (3) introducing a F127 copolymer into an (MAFA)CsPbIBr system; (4) depositing an (MAFA)CsPbIBr photosensitive layer on the KI interface modification layer; (5) depositing a Spiro-OMeTAD hole transport layer on thephotosensitive layer; and (6) evaporating an Au counter electrode on the Spiro-OMeTAD hole transport layer. The average photoelectric conversion efficiency of the solar cell prepared by the method reaches 22.72%, the highest photoelectric conversion efficiency exceeds 22.85% by optimizing a device preparation process, and the solar cell has good illumination stability.

The invention relates to an optical fiber electric field sensor based on the F-P interference principle. The sensor comprises an optical fiber electric field sensor body which is connected with an adjustable direct-current voltage source and an optical fiber sensor demodulator. The optical fiber sensor demodulator is connected with a computer. The manufacturing method of the optical fiber electricfield sensor comprises the following steps: inserting an optical fiber into a ceramic contact pin, and cutting a plastic plate into a circle with the same diameter as the piezoelectric ceramic piece;drilling a hole in the center of the plastic plate, and inserting the ceramic contact pin into the hole in the plastic plate; and arranging the piezoelectric ceramic piece and the plastic plate withthe ceramic contact pin in a stainless steel tube. The method for measuring by using the optical fiber electric field sensor comprises the following steps: introducing a direct-current voltage source;when the piezoelectric ceramic piece is subjected to an external electric field, enabling the piezoelectric ceramic piece to be subjected to telescopic deformation in the thickness direction; transmitting the data to the computer through a demodulator to obtain the variable quantity of the cavity length; and solving the electric field intensity according to the deformation quantity and an inversepiezoelectric coefficient. The sensor has the advantages of simple structure, small size, high precision and high response speed.

The invention relates to a preparation method of dendrimer stable bismuth sulfide nanoparticles. The preparation method comprises: (1) preparing a diluted acetic acid solution of hydroxyl-terminated fourth-generation polyamide-amine dendrimer and preparing a bismuth salt diluted acetic acid solution; and (2) mixing the diluted acetic acid solution of hydroxyl-terminated fourth-generation polyamide-amine dendrimer with the bismuth salt diluted acetic acid solution, introducing H2S gas, reacting under the protection of nitrogen gas for 1-2 hours, carrying out dialysis after the reaction is finished, and performing freeze-drying to obtain the dendrimer stable bismuth sulfide nanoparticles. The preparation method provided by the invention is simple to operate and has mild reaction conditions; and the prepared dendrimer stable bismuth sulfide nanoparticles have the advantages of narrow particle size distribution, good dispersion property and no agglomeration, can be potentially used in CT (computed tomography) imaging diagnosis, and has been successfully applied to the rabbit in-vivo imaging.

The invention relates to a device for measuring deformation quantity of an electrostrictive material based on a Fabry-Perot cavity. The device comprises a base and a ball screw; the ball screw and a guide rail are vertically fixed to the base and connected through a sliding block. The fixed ball screw is connected with a lifting controller through a stepping motor; a material test bench is arranged on the base; a material clamp is arranged above the material test bench; an optical probe is fixed on a connecting plate through a fixing device and is connected with an external leading-out opticalfiber, and the end surface of the optical probe faces the surface of a fixed piezoelectric film; an optical signal emitted by a laser light source is processed to form parallel light, the optical signal is incident to the piezoelectric film through the optical fiber and reflected on the upper surface of the piezoelectric film, the optical signal is transmitted to an optical demodulation system through the optical fiber, and deformation generated on the piezoelectric film is sensed through change of an optical phase. The device has the advantages of high measurement precision, simple structure, strong anti-interference capability, high response speed and small size.

The invention discloses a method for rapidly carrying out variety analysis and content determination on anthocyanin in tomato fruits. Methanol / formic acid (9: 1, v / v) is used as a solvent; after an internal standard is added, anthocyanin in tomato fruits is subjected to liquid-liquid extraction, extraction is conducted for 12 h at a temperature of 4 DEG C, and supernate is taken through centrifugation; after membrane passing is conducted, mass spectrum information and spectral information of anthocyanin are collected at the same time, the anthocyanin in the tomato fruits is quantified, and structural analysis is conducted. According to the detection method, the pretreatment process is simple, the detection result is accurate, and tedious pretreatment purification steps are not needed. Themethod can be used for qualitatively and quantitatively analyzing anthocyanin in tomato fruits.

The invention relates to a recombinant lentiviral vector aiming at hNINL gene RNA (Ribonucleic Acid) interference and preparation thereof. The lentiviral vector aiming at ShRNA of the hNINL gene is experimentally constructed; a synthetic DNA (Deoxyribonucleic Acid) segment aiming at the ShRNA is mediated through the lentiviral vector, and is in cotransfection 293 T cell culture with two vectors of pHelper 1.0 and pHelper 2.0; and after the recombinant lentiviral vector is obtained, a target cell is cotransfected, so that RNA interference aiming at the hNINL gene is realized. The adopted inactive third-generation lentiviral vector (SIN) has the advantages of safety, reliability, capability of infecting nondividing cells, long-duration expression of integrating target genes into target cell gene groups, small immune reaction and the like, and is an ideal vector. According to the lentiviral vector, the interference effect on human breast cancer cells MCF-7 reaches 60-85 percent, so that the recombinant lentiviral vector lays a good experimental foundation for further research of relevant hNINL genes, and can be widely used for in vivo gene treatment and gene function research.

The invention relates to a recombinant lentivirus-based vector for implementing the RNA (Ribose Nucleic Acid) interference aiming at an FLG (filaggrin) gene and preparation of the recombinant lentivirus-based vector. A lentivirus-based vector of shRNA (shorthairpin RNA) aiming at the FLG gene is constructed through experiments; a synthesized DNA fragment aiming at the ShRNA is mediated by the lentivirus-based vector; 293T cells are subjected to co-transfection together by the lentivirus-based vector and two vectors pHelper1.0 and pHelper2.0 to carry out culture; and after the recombinant lentivirus-based vector is obtained, target cells are subjected to transfection so as to implement the RNA interference aiming at the FLG gene. The adopted self-inactivated third-generation lentivirus-based vector has the advantages of safety, reliability, capability of infecting nondividing cells, long-term expression of integrating a target gene to a target cell gene, small immune response and the like and is an ideal vector. The lentivirus-based vector disclosed by the invention has the interference effect reaching 75 to 95 percent on normal skin cells HACAT of people, and thus, the recombinant lentivirus-based vector disclosed by the invention lays a good experimental foundation for further research related to the FLG gene and can be widely used for the in-vivo gene therapy and the gene functional research.

A method for constructing multifunctional gene therapy vectors based on gold nanoparticles. The invention belongs to the technical field of CT contrast agents and non-viral gene carriers, and specifically relates to gold nanoparticles as the skeleton, including spherical gold nanoparticles, rod-shaped gold nanoparticles (with different axis-to-diameter ratios), arrowhead-shaped gold nanoparticles, and octahedral gold nanoparticles. The morphology of gold nanoparticles, etc., using modified bovine serum albumin to wrap gold nanoparticles, and ATRP method to construct a multifunctional gene carrier. The polymerization reaction is stable and easy to control, and a variety of high-performance cationic gene carriers with different monomer contents can be prepared according to needs, and the storage stability is good, and the original performance can be maintained after being placed for several days or months. The cationic gene carrier has a higher transfection efficiency than the international "gold standard" PEI in HepG2, COS7, 293, C6 and other cells, and its cytotoxicity is much lower than that of PEI. It is simple to use and has commercial potential. And the material has a good CT imaging effect, which lays a good experimental foundation for the development of new contrast agents.

The invention provides an electric field sensor based on an electrostriction effect. The electric field sensor includes an electrostriction ceramic block shell, a groove body is arranged in the electrostriction ceramic block shell, a strain gauge is arranged in the groove body, the strain gauge comprises a strain gauge substrate, a grid structure is arranged on the strain gauge substrate, and thegrid structure is connected with one end of the connecting wire through an end point, and the other end of the connecting wire is connected with a bridge circuit, and the strain gauge is attached to the wall of the inner groove body of the electrostriction ceramic block shell through the adhesive. The invention aims to provide an electric field sensor which is simple in structure, small in size, low in power consumption, and can better realize the electric field intensity measurement.

The invention relates to a preparation and detection method of pancreatic regenerating protein PSP / reg. The preparation and detection method specifically comprises the steps that an adult healthy malesprague-dawley (SD) rat is adopted and treated with a single injection of dibutyltin dichloride in caudal vein to construct a chronic pancreatitis rat model, high fructose is given for feeding, a type 3c diabetes (T3cDM) rat model is established, and then detection of related physiological and biochemical indexes is performed. It is verified that the clinical physiological and biochemical changesof the T3cDM rat model established are consistent with the clinical physiological and biochemical changes of T3cDM. The rat model can establish an experimental basis for the drug screening and clinical treatment of T3cDM and provides experimental tools. Meanwhile, the pancreatic regenerating protein PSP / reg further provides a good experimental gist for the diagnosis or treatment of T3cDM.

The invention relates to a recombinant lentivirus-based vector for implementing the RNA (Ribose Nucleic Acid) interference aiming at an FLG (filaggrin) gene and preparation of the recombinant lentivirus-based vector. A lentivirus-based vector of shRNA (shorthairpin RNA) aiming at the FLG gene is constructed through experiments; a synthesized DNA fragment aiming at the ShRNA is mediated by the lentivirus-based vector; 293T cells are subjected to co-transfection together by the lentivirus-based vector and two vectors pHelper1.0 and pHelper2.0 to carry out culture; and after the recombinant lentivirus-based vector is obtained, target cells are subjected to transfection so as to implement the RNA interference aiming at the FLG gene. The adopted self-inactivated third-generation lentivirus-based vector has the advantages of safety, reliability, capability of infecting nondividing cells, long-term expression of integrating a target gene to a target cell gene, small immune response and the like and is an ideal vector. The lentivirus-based vector disclosed by the invention has the interference effect reaching 75 to 95 percent on normal skin cells HACAT of people, and thus, the recombinant lentivirus-based vector disclosed by the invention lays a good experimental foundation for further research related to the FLG gene and can be widely used for the in-vivo gene therapy and the gene functional research.

The invention relates to a CT / MR / near infrared optical three-mode targeted contrast agent based on the lung non-cellule cancer as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, modifying the amino terminal of a fifth-generation polyamide-amine dendrimer with DOTA-NHS; secondly, connecting folic acid to the dendrimer by polyethylene glycol; thirdly, further modifying with Cy5.5; fourthly, preparing gold nanoparticles by using the modified dendrimer as a template through sodium borohydride reduction, and chelating gadolinium ions to the dendrimer; fifthly, carrying out complete acetylization on amino remained on the surface of the dendrimer; and finally, dialyzing the reacted solution, lyophilizing to obtain a final product, and evaluating in-vivo and in-vitro CT / MR / near infrared optical three-mode contrast performance of the product. The method is simple and feasible, has excellent in-vivo and in-vitro lung non-cellule cancer cell target effect, and has a potential application prospect of targeted CT / MR / near infrared optical contrast.

The invention belongs to the technical field of traditional Chinese medicine anticancer treatment and particularly relates to a traditional Chinese medicine extract composition, a melanoma inhibitingdrug, a preparation method and application. The traditional Chinese medicine extract composition comprises a ligustrum lucidum alcohol extract and a herba ecliptae alcohol extract which are in a crudedrug ratio of (0.5-1.5): 1. According to the traditional Chinese medicine extract composition, the action of inhibiting activity of melanoma cells is exerted through inhibiting cell cycle G2 / M-stagetime phase, cell proliferation and cell cloning, so that the apoptosis of the melanoma cells is promoted, a good experiment foundation is settled for traditional Chinese medicine treatment on tumors such as malignant melanoma, and a new view is developed. The traditional Chinese medicine extract composition can be used for preparing drugs for inhibiting melanoma and is applied to treatment or deepresearches on the melanoma.

The invention relates to a graphene / metal / MgB2 composite material with high intergranular connectivity and a preparation method thereof. Mg, B and a certain proportion of graphene and metal are jointly subjected to ball milling, and finally, an MgB2 superconducting block body is obtained through high-temperature sintering. The MgB2 superconducting block body prepared through the method is good in intergranular connectivity, thin and small in grain and even in distribution, and forms an eutectic liquid phase with the Mg at the low temperature through doping of Cu or Ni, favorable conditions are provided for diffusion and reaction between Mg and B particles, the reaction free energy is reduced, and the formation temperature of MgB2 is decreased to 500 DEG C. A reaction site is further provided for the particles through adding of the graphene, and thus MgB2 particles are subjected to nucleation and growth on a graphene carrier, so that the MgB2 forms a network structure, and the intergranular connectivity is improved. The used raw materials are easy to obtain, the material preparation method is mature in development, operation is convenient, and the process is controllable; and according to the method, the MgB2 reaction temperature is effectively decreased, and the intergranular connectivity of the MgB2 is improved.

The invention relates to a CT / MR / near infrared optical three-mode targeted contrast agent based on the lung non-cellule cancer as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, modifying the amino terminal of a fifth-generation polyamide-amine dendrimer with DOTA-NHS; secondly, connecting folic acid to the dendrimer by polyethylene glycol; thirdly, further modifying with Cy5.5; fourthly, preparing gold nanoparticles by using the modified dendrimer as a template through sodium borohydride reduction, and chelating gadolinium ions to the dendrimer; fifthly, carrying out complete acetylization on amino remained on the surface of the dendrimer; and finally, dialyzing the reacted solution, lyophilizing to obtain a final product, and evaluating in-vivo and in-vitro CT / MR / near infrared optical three-mode contrast performance of the product. The method is simple and feasible, has excellent in-vivo and in-vitro lung non-cellule cancer cell target effect, and has a potential application prospect of targeted CT / MR / near infrared optical contrast.