Preparation of dendrimer/ gold-silver alloy nanoparticle with computed tomography (CT) radiography function

A dendrimer and nanoparticle technology, which can be used in preparations for in vivo experiments, MRI/MRI contrast agents, emulsion delivery, etc., can solve the problem of not finding gold-silver alloy nanoparticles, etc. Good use value, narrow size distribution, easy to operate effect

Inactive Publication Date: 2011-03-23
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Retrieval of literature and patents related to gold-silver alloy nanoparticles at home and abroad shows that: before the completion of the present invention, no report on the application of gold-silver alloy nanoparticles based on dendrimers in CT imaging has been found.

Method used

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  • Preparation of dendrimer/ gold-silver alloy nanoparticle with computed tomography (CT) radiography function
  • Preparation of dendrimer/ gold-silver alloy nanoparticle with computed tomography (CT) radiography function
  • Preparation of dendrimer/ gold-silver alloy nanoparticle with computed tomography (CT) radiography function

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Take 299.52mgAgNO 3 , dissolved in distilled water, magnetically oscillated to fully dissolve and evenly prepare transparent and colorless Ag with a concentration of 88.16mmol / L + 20 mL of saline solution. Take 114.68mg of NaBH 4 , dissolved in distilled water / methanol (water:methanol=2:1, volume ratio), and magnetically oscillated to fully dissolve and evenly prepare 4.2mL of a solution with a concentration of 721.78mmol / L. Take the fifth generation polyamide-amine dendrimers (G5.NH 2 ) 48.65 mg, dissolved in 5 mL of distilled water, and magnetically oscillated to fully dissolve and evenly prepare a solution with a concentration of 9.73 mg / mL. Take AgNO 3 Solution 1.485mL, added to G5.NH 2 Solution, stirred for 0.5h. After that, add NaBH 4 Solution 0.544mL, stirring reaction 2h. After the reaction, half of the solution was dialyzed, PBS buffer solution (3 times, 4L / time) and distilled water (3 times, 4L / time). Then freeze-drying was carried out to obtain a den...

Embodiment 2

[0051] Take 299.52mgAgNO 3 , dissolved in distilled water, magnetically oscillated to fully dissolve and evenly prepare transparent and colorless Ag with a concentration of 88.16mmol / L + 20 mL of saline solution. Take HAuCl 4 50 mg, dissolved in distilled water, magnetic shock to fully dissolve it evenly, and prepare a solution with a concentration of 10 mg / mL. Take 114.68mg of NaBH 4 , dissolved in distilled water / methanol (water:methanol=2:1, volume ratio), and magnetically oscillated to fully dissolve and evenly prepare 4.2mL of a solution with a concentration of 721.78mmol / L. Take the fifth generation polyamide-amine dendrimers (G5.NH 2 ) 50.35mg, dissolved in 5mL of distilled water, and magnetically oscillated to fully dissolve and evenly prepare a solution with a concentration of 10.07mg / mL. Take AgNO 3 Solution 1.098mL, added to G5.NH 2 solution, followed by the addition of HAuCl 4 Solution 1.595mL, stirred for 0.5h. After that, add NaBH 4 The solution was 0.5...

Embodiment 3

[0056] Take 240.70mg AgNO 3 , dissolved in distilled water, and magnetically oscillated to fully dissolve and evenly prepare transparent and colorless Ag with a concentration of 70.84mmol / L + 20 mL of saline solution. Take HAuCl 4 50 mg, dissolved in distilled water, magnetic shock to fully dissolve it evenly, and prepare a solution with a concentration of 10 mg / mL. Take 56.26mg of NaBH 4 , dissolved in distilled water / methanol (water:methanol=2:1, volume ratio), and magnetically oscillated to fully dissolve and evenly prepare 2.1mL of a solution with a concentration of 708.18mmol / L. Take the fifth generation polyamide-amine dendrimers (G5.NH 2 ) 57.13 mg, dissolved in 5 mL of distilled water, and magnetically oscillated to fully dissolve and evenly prepare a solution with a concentration of 11.43 mg / mL. Take AgNO 3 Solution 1.090mL, added to G5.NH 2 solution, followed by the addition of HAuCl 4 Solution 3.166mL, stirred for 0.5h. After that, add NaBH 4The solution w...

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Abstract

The invention relates to an in-situ preparation method of dendrimer / gold-silver alloy nanoparticle with a computed tomography (CT) radiography function, comprising the following steps: (1) weighing the fifth generation of polyamide-amine dendrimer of which the tail end is an amino, and preparing 9.7-14.9mg / mL of solution; and (2) in the above solution, adding silver nitrate solution, then addingchloroauric acid solution, adding sodium borohydride solution after stirring, and continuing stirring and reacting for 2-3 hours, dialyzing the reacted solution, freezing and drying to obtain an acetylated pre-product. The dendrimer / gold-silver alloy nanoparticle prepared by the method of the invention has narrow size distribution, good dispersibility and no agglomeration phenomenon. The X-ray attenuation intensity test shows that the X-ray attenuation intensity of alloy nanoparticle is in rising tendency and gradually shows the CT imaging property superior to that of the traditional contrast agent Iohexol.

Description

technical field [0001] The invention belongs to the field of preparation of nanoparticles, in particular to an in-situ preparation method of dendrimers / gold-silver alloy nanoparticles with CT imaging function. Background technique [0002] Noble metal nanoparticles have shown their potential application value in many fields due to their unique optical, electrical, catalytic and quantum size-related properties, which have aroused strong research interest of researchers. Among them, gold nanoparticles and silver nanoparticles are favored by researchers because of their remarkable properties of nanostructures. Shi X.Y. et al [Shi, X.Y.; Wang, S.H.; Meshinchi, S.; Van Antwerp, M.E.; Bi, X.D.; Lee, I.H.; ]. Small 2007, 3, 1245.] Gold nanoparticles with an average size of 3.2nm were prepared by in situ reduction method. The prepared gold nanoparticles have good biocompatibility and can effectively target and image cancer cells. Sutton, A. et al [Sutton, A.; Franc, G.; Kakkar, A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/12A61K49/06
Inventor 史向阳刘辉
Owner DONGHUA UNIV
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