Photoresponse silicon oxide nanometer vector and preparation method thereof

Abstract

The invention belongs to the technical field of a non-viral gene vector and a medicine / photosencitizer vector, and particularly relates to a multifunctional nanometer vector and a preparation method of the multifunctional nanometer vector, wherein the multifunctional nanometer vector is built by using silicon oxide nanometer particles as substrates, functionalizing the silicon oxide nanometer particles through surface modification, introducing photosensitive groups with ultraviolet / near infrared light response and using an ATRP (Atom Transfer Radical Polymerization) method. The multifunctionalnanometer vector has higher transfection efficiency than international JINBIAO PEI in cells of HepG2, COS7, 293, C6, Hela and the like, and has photoresponse gene release performance. Under the photostimulation, the photosensitive groups on the surfaces of the silicon oxide nanometer particles perform photolysis, so that positive ions fall off, and the genes are released; and the gene transfection efficiency can be greatly improved. Various different medicine or photosencitizer molecules can be loaded into porous structures of mesoporous silicon oxide nanometer particles; and good experimentfoundation is laid for the development of the novel photoresponse nanometer vector. The method has the advantages that the use method is simple; and the commercialization potential is realized.

Description

(1) Technical field

[0001] The invention belongs to the technical field of non-viral gene carrier and drug / photosensitizer carrier, and specifically relates to the use of silicon oxide nanoparticles as a substrate, the use of surface-modified functionalized silicon oxide nanoparticles, and the introduction of photosensitive groups with ultraviolet / near-infrared photoresponses. A multifunctional nanocarrier constructed by atom transfer radical polymerization (ATRP) and a preparation method thereof. (2) Background technology

[0002] In recent years, malignant tumors have become the main killer that threatens human life and health, and the treatment of malignant tumors has become a worldwide problem that needs to be solved urgently, which has extremely important strategic significance. Due to the influence of many adverse factors, such as recurrence after surgery, strong toxic and side effects of radiotherapy and chemotherapy, the commonly used tumor treatment methods in clini...

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