A method for constructing multifunctional gene therapy vectors based on gold nanoparticles
A gold nanoparticle and gene therapy technology, applied in gene therapy, preparations for in vivo experiments, pharmaceutical formulations, etc., can solve the problems of increased endocytosis, increased cytotoxicity, and increased transfection efficiency, achieving The effect of stable reaction, good storage stability and easy regulation
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Examples
Embodiment 1
[0033] 1) Synthesis of gold nanoparticles:
[0034] Seeding solution: To cetyltrimethylammonium bromide solution (2.5 mL, 0.2 M) was added 74 μL of 0.024 M HAuCl 4 solution, then add 1.0458mLH 2 O, mix well; then add 0.5 mL of 10 mM NaBH kept in ice water 4 solution, stirred vigorously for 2 minutes; the solution turned brownish yellow, stored at 27°C for 1 hour before taking;
[0035] Growth solution: To cetyltrimethylammonium bromide solution (25 mL, 0.2 M) was sequentially added 1.4 mL of AgNO at a concentration of 4 mM 3 solution, add 1.5mL of 0.024M HAuCl 4 solution, then add 21mLH 2 O; each solution should be shaken and mixed evenly after adding; then add 620 μL ascorbic acid (0.0788M), the solution turns from dark yellow to colorless; finally add 500 μL seed solution, react at 27 ° C for 24 h; centrifuge at 2000 rpm for 10 min , then take the supernatant and continue to centrifuge at 12000rpm for 20min to obtain rod-shaped gold nanoparticles;
[0036] 2) Bovine se...
Embodiment 2
[0041] The reaction time of step 4) in Example 1 was 10 minutes, and the rest of the reaction conditions were the same as in Example 1. The obtained multifunctional gene therapy carrier based on gold nanoparticles was designated as Au-pDMA2.
Embodiment 3
[0043] The reaction time of step 4) in Example 1 was 30 minutes, and the rest of the reaction conditions were the same as in Example 1. The obtained multifunctional gene therapy carrier based on gold nanoparticles was designated as Au-pDMA3.
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Application Information
- IPC
- A61K48/00; A61K47/02; A61K49/18; A61K49/14
- Inventors
- 徐福建; 赵娜娜