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Preparation and detection method of pancreatic regenerating protein PSP/reg

A technology of pancreas and rat models, applied in the field of biomedicine, can solve problems such as difficulty in the pathogenesis of T3cDM

Active Publication Date: 2019-03-01
ZHONGDA HOSPITAL SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few animal models of T3cDM in the prior art, which brings great difficulties to the study of the pathogenesis of T3cDM

Method used

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  • Preparation and detection method of pancreatic regenerating protein PSP/reg
  • Preparation and detection method of pancreatic regenerating protein PSP/reg
  • Preparation and detection method of pancreatic regenerating protein PSP/reg

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, establishment of chronic pancreatitis (chronic pancreatitis, CP) rat model 1, grouping of rats

[0030] Eighty adult healthy male SD rats, each weighing 200-250 g, were randomly divided into 4 groups, 20 in each group. Grouping includes: (1) normal control group; (2) high fructose treatment control group; (3) model control group; (4) high fructose treatment model group.

[0031] Experimental animals were purchased from the Model Animal Center of Nanjing University, and were reared in single cages under SPF conditions in the animal room of the school, with regular feed and drinking water, 12 hours of day and night alternation, the ambient temperature and humidity were suitable, and the feeding environment was clean.

[0032] 2. Establishment of CP rat model

[0033] The rats in the model group (3) model control group and (4) high fructose treated model group were treated with dibutyltin dichloride (DBTC). The CP rat model was established by a single injec...

Embodiment 2

[0037] Embodiment 2, establishment of secondary T3cDM rat model

[0038] 1. Grouping of rats

[0039] According to the 4 groups divided in Example 1.

[0040] 2. Establishment of secondary T3cDM rat model

[0041] After 4 weeks of DBTC-treated rats, the tail vein injection of DBTC and saline was stopped.

[0042]Rats in (1) the normal control group and (3) the rats in the model control group were given a normal diet, respectively.

[0043] For (2) high-fructose-treated control group and (4) high-fructose-treated model group, in addition to the normal diet, additional high-fructose (Research Diets, Inc. New Brunswick, USA) was given to induce insulin resistance and establish a T3cDM rat model . The amount of high fructose is 20% of the normal dietary amount.

[0044] Each experimental group was continuously fed for 8 weeks, and the animals were sacrificed at the 2nd, 4th, 6th and 8th week after high fructose treatment, and the process was completed within 2 minutes.

Embodiment 3

[0045] Embodiment 3, index detection of secondary T3cDM rat model

[0046] 1. Experimental method

[0047] 1. The body weight of each experimental group was monitored weekly, glucose tolerance was measured by OGTT, blood was drawn to measure fasting blood glucose and insulin, and pancreatic β-cell function and insulin resistance levels were evaluated. Carry out according to the conventional experimental method in this technical field or refer to the instructions of detection kits and experimental instruments, and the following experimental methods are similar.

[0048] 2. Use CT / MRI to dynamically observe the pancreas volume, pancreatic duct dilatation, cyst formation, pancreatic calcification and surrounding tissue, and evaluate the relationship between the range and degree of CP and T3cDM.

[0049] 3. The endocrine function of each experimental group was detected, and the serum PP level was detected by ELISA method.

[0050] 4. The level of pancreatic stress protein was de...

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Abstract

The invention relates to a preparation and detection method of pancreatic regenerating protein PSP / reg. The preparation and detection method specifically comprises the steps that an adult healthy malesprague-dawley (SD) rat is adopted and treated with a single injection of dibutyltin dichloride in caudal vein to construct a chronic pancreatitis rat model, high fructose is given for feeding, a type 3c diabetes (T3cDM) rat model is established, and then detection of related physiological and biochemical indexes is performed. It is verified that the clinical physiological and biochemical changesof the T3cDM rat model established are consistent with the clinical physiological and biochemical changes of T3cDM. The rat model can establish an experimental basis for the drug screening and clinical treatment of T3cDM and provides experimental tools. Meanwhile, the pancreatic regenerating protein PSP / reg further provides a good experimental gist for the diagnosis or treatment of T3cDM.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for preparing and detecting pancreatic regeneration protein PSP / reg, including a method for preparing a type 3c diabetes T3cDM rat model and the prepared T3cDM rat model. Background technique [0002] Diabetes mellitus (DM) has become the third chronic disease that seriously endangers human health after tumors and cardiovascular diseases. Among them, diabetes caused by pancreatic exocrine diseases is classified as a special type of diabetes, that is, type 3c diabetes (T3cDM), also known as pancreatic-derived diabetes. T3cDM is mostly caused by the reduction of islet cells caused by pancreatic exocrine injury. The main symptoms are hypoinsulinemia, decreased pancreatic polypeptide (pancreatic polypeptide, PP) level, increased peripheral insulin sensitivity, and hepatic insulin resistance. Studies have found that T3cDM accounts for 5% to 10% of all diabetic patients, and chroni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/32A61K49/00A61K39/395A61P3/10A01K67/02
CPCA01K67/02A61K31/32A61K49/0008A61K2039/505A61P3/10C07K16/18
Inventor 李玲林昊王昆魏琼祝祥云
Owner ZHONGDA HOSPITAL SOUTHEAST UNIV
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