Optical three-mode targeted contrast agent for lung non-cellule cancer as well as preparation method and application thereof
A non-small cell cancer, targeted contrast agent technology, applied in the direction of X-ray contrast agent preparation, general/multifunctional contrast agent, pharmaceutical formulations, etc., can solve the problem of non-small cell carcinoma of the lung not found in contrast performance and other issues
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0074] First, FA-PEG-COOH was synthesized according to previous studies.
[0075] Take the fifth generation polyamide-amine dendrimers (G5.NH 2 ) 81.47mg, dissolved in 20mL DMSO to obtain a solution, then DOTA-NHS (23.85mg) dissolved in DMSO (10mL) was added dropwise to the above solution, and stirred with strong magnetic force for 24h to obtain G5.NH2-DOTA. FA-PEG-COOH (61.04 mg) was activated by EDC (1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride) (48.04 mg dissolved in 10 mL DMSO) for 3 hours, and gradually Add G5.NH dropwise 2 -DOTA solution, followed by strong magnetic stirring for 3 days, the obtained G5.NH2-DOTA-(PEG-FA) was further modified by mPEG-COOH (93.96 mg) that had been activated by EDC for 3 hours, and G5 was obtained after magnetic stirring for 3 days .NH 2 -DOTA-(PEG-FA)-mPEG dendrimer.
[0076] Such as figure 2 as shown, 1 H NMR spectrum proves that mPEG-COOH has been successfully modified in G5.NH 2 On the surface of the dendrimer, each...
Embodiment 2
[0078] With the G5.NH in embodiment 1 2 -DOTA-(PEG-FA)-mPEG dendrimers were further modified with 3 molar equivalents of Cy5.5 (6.73 mg) and stirred under strong magnetic force for 1 h in the dark to obtain G5.NH 2 -DOTA-(PEG-FA)-mPEG-Cy5.5.
[0079] Such as image 3 As shown, the absorption peak at 650nm in the ultraviolet spectrum is the absorption peak of Cy5.5, which proves that Cy5.5 is successfully conjugated on the surface of the dendrimer.
Embodiment 3
[0081] Get the G5.NH prepared in Example 2 2 -DOTA-(PEG-FA)-mPEG-Cy5.5 dendrimer solution, add 5.72 mL (30 mg / mL) of chloroauric acid solution, and stir for 30 minutes. Then add frozen sodium borohydride solution (78.98mg, 10mL) loaded with more than 5 times the mole of gold particles, and stir for several minutes until the color of the reaction mixture turns to wine red, suggesting that the gold nanoparticles have been synthesized on the macromolecules. The reaction mixture was stirred for 2 h to complete the reaction. After that, the Gd(NO 3 ) 3 Aqueous solution (32.80 mg, 1 mL) and 35 molar equivalents of G5 dendrimers were added dropwise to the above mixture, stirred for 24 h to obtain {(Au°)200-G5.NH 2 -DOTA(Gd)-(PEG-FA)-mPEG-Cy5.5}DENPs.
[0082] After the reaction, 160.0 uL of triethylamine was added, and magnetically stirred for 30 min, then, 130.3 uL of acetic anhydride was added, and the reaction was stirred for 24 h. After the reaction, the reaction mixture was...
PUM
Property | Measurement | Unit |
---|---|---|
Concentration | aaaaa | aaaaa |
Hydrated particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com