Construction and application of farnesyl pyrophosphate synthase RNA interference recombinant lentiviral vector

A technology of farnesyl pyrophosphate and recombinant lentivirus, which is applied in the field of molecular biology and can solve the problem that non-viral vectors cannot satisfy long-term expression
CN101805750BInactive Publication Date: 2011-11-30ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2011-11-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides the construction of a recombinant lentiviral vector targeting farnesyl pyrophosphate synthase RNA interference. The most effective target sequence of FDS gene RNAi is screened out in the tool cell 293T cells, and the double-stranded DNA of the most effective target sequence is synthesized and connected to pGCSIL - GFP vector, the recombinant vector was successfully constructed through restriction sequencing. Studies have shown that the constructed RNA interference vector LV-sh-FDS can down-regulate the expression of FDS mRNA level in neonatal rat cardiomyocytes, and can also down-regulate the expression of cardiac hypertrophy markers such as cell area and marker genes beta-MHC and BNP. While down-regulating FDS, it can also effectively inhibit the activity of RhoA, and can be used in the preparation of drugs for treating cardiac hypertrophy diseases. It can also be used in the preparation of drugs for regulating cholesterol metabolism.
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Description

technical field

[0001] The invention belongs to the technical fields of molecular biology, biomedicine and genetic engineering, and mainly relates to the construction of an RNA interference recombinant lentiviral vector (LV-sh-FDS) targeting the gene of Farnesyl pyrophosphate synthase (FDS) and its Applications in cardiac hypertrophy and regulation of cholesterol metabolism. Background technique

[0002] Current research shows that small G protein is involved in the occurrence of cardiac hypertrophy. RhoA belongs to the superfamily of small G proteins, and has two forms: a GDP-bound inactive state and a GTP-bound active state. At present, many studies have shown that RhoA is involved in the occurrence of cardiac hypertrophy, such as angiotensin II (Ang II)-induced cardiac hypertrophy. Studies have found that overexpression of Rho-GDI (Rho-GDP separation inhibitor) and Rho inhibitor C3exeoezyme can significantly inhibit Ang II-induced cardiac hypertrophy.

[0003] Farnesyl...

Claims

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