Drug-resistant pathogen infection resistant polypeptide and uses thereof

An antibacterial polypeptide and drug resistance technology, applied in the field of biomedicine, can solve the problems of accelerating the evolution of pathogenic bacteria and increasing the rate of bacterial drug resistance, and achieve the effect of high antibacterial activity

Inactive Publication Date: 2012-10-03
CHINA PHARM UNIV
View PDF2 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of a large number of broad-spectrum antibiotics has also strengthened the screening effect on pathogenic bacteria, accelerated the evolution of pathogenic bacteria, gradually increased the drug resistance rate of bacteria, and expanded the drug resistance spectrum, eventually leading to the emergence of super drug-resistant bacteria. Appear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Drug-resistant pathogen infection resistant polypeptide and uses thereof
  • Drug-resistant pathogen infection resistant polypeptide and uses thereof
  • Drug-resistant pathogen infection resistant polypeptide and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Synthesis and structure confirmation of peptides:

[0028] The polypeptide of the present invention is artificially synthesized by conventional solid-phase synthesis method. Polypeptide Cbf-K of the present invention 16 and Cbf-A 7 A 13 , its purity determined by HPLC is greater than 99%, and its molecular weight determined by mass spectrometry is 3637.63 and 3496.37 respectively, and its molecular weight is consistent with the theoretical value of 3638.1 and 3496.8.

Embodiment 2

[0030] Polypeptide Cbf-K of the present invention 16and Cbf-A 7 A 13 In vitro antibacterial effect on recombinant E.coli NDM-1:

[0031] (1) Accurately weigh the polypeptide Cbf-K 16 and Cbf-A 7 A 13 Prepare a drug solution with a concentration of 1280 μg / ml, filter it aseptically with a 0.22 μm membrane, aliquot it, and store it at -70°C for later use.

[0032] (2) Inoculate recombinant E.coli NDM-1 on the slant of nutrient agar, incubate at 37°C for 18h, then pick a little and inoculate it into 2ml nutrient broth medium, incubate at 37°C for 8h, dilute with sterile MH broth into 10 5 Bacterial suspension with about CFU / ml was continuously induced with IPTG.

[0033] (3) Take a sterile 96-well plate, add 40 μl of drug stock solution and 60 μl of MH broth medium to the first column, add 50 μl of MH broth medium to each well of the remaining wells, and dilute the drug in turn to 50 μl containing drug in each row Solutions with concentrations of 512, 256, 128, 64, 32, 16...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of biomedicine, in particular to an antimicrobial polypeptide of which the amino acid sequence is shown as SEQ ID: NO: 1 or as SEQ ID: NO: 2. The antimicrobial polypeptide provided by the invention has the uses of resistance to drug-resistant pathogen infection, particularly resistance to drug-resistant super bacteria (super bacteria).

Description

technical field [0001] The invention relates to the field of biomedical technology, in particular to an antibacterial polypeptide. The antibacterial polypeptide of the invention has the application of resisting drug-resistant pathogenic bacteria infection, especially super-drug-resistant bacteria (super bacteria). Background technique [0002] The discovery of antibiotics has brought people into the era of controlling and treating bacterial infectious diseases. However, the use of a large number of broad-spectrum antibiotics has also strengthened the screening effect on pathogenic bacteria, accelerated the evolution of pathogenic bacteria, gradually increased the drug resistance rate of bacteria, and expanded the drug resistance spectrum, eventually leading to the emergence of super drug-resistant bacteria. Appear. Super drug-resistant bacteria (superbugs) are spreading across the globe. It has been reported that superbugs are insensitive to currently clinically used antib...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00A61K38/16A61P31/04
Inventor 周长林窦洁王晶郝青茹王慧周伟东
Owner CHINA PHARM UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap