A method of inducing Haematococcus pluvialls to produce astaxanthin

A technology of Haematococcus pluvialis and astaxanthin, which is applied in the field of microorganism application, can solve problems such as the inability to meet high-efficiency utilization, and achieve the effects of increasing yield, shortening time, and simple and easy method.

Active Publication Date: 2017-10-20
BIOALGO CY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previously, natural astaxanthin was derived from krill oil, crayfish oil, and Phaffia rhodozyme. The content of astaxanthin in these sources was only between 0.15% and 0.4%, which could not meet the market's demand for efficient utilization

Method used

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  • A method of inducing Haematococcus pluvialls to produce astaxanthin
  • A method of inducing Haematococcus pluvialls to produce astaxanthin

Examples

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Embodiment 1

[0029] Step 1. Massive propagation of algae species: inoculate Haematococcus pluvialis into the nutrient medium at a temperature of 22°C and a continuous light intensity of 20 μmol·m -2 ·s -1 And at the same time, continue to pass into the nutrient medium through filtering under the air condition of 1% carbon dioxide with a volume concentration, and its feed rate is to pass into 0.2L / min in every 1L of the nutrient medium, until Reach the end of the logarithmic growth phase of Haematococcus pluvialis, and obtain a large number of algae species;

[0030] Step 2. Production of astaxanthin: Inoculate Haematococcus pluvialls into the production medium, and produce astaxanthin at a temperature of 22°C, wherein the light on Haematococcus pluvialls is continuously maintained during the cultivation process, and Ensure that the light intensity reaching the cell surface of Haematococcus pluvialis is 20 μmol m -2 ·s -1 Adding ethanol to the nutrient medium at the same time, wherein th...

Embodiment 2

[0033] Step 1. Massive propagation of algae species: inoculate Haematococcus pluvialis into the nutrient medium at a temperature of 24°C and a continuous light intensity of 60 μmol·m -2 ·s -1 And at the same time, continue to pass into the nutrient medium through filtering under the air condition of 1% carbon dioxide with a volume concentration, and its feed rate is to pass into 0.2L / min in every 1L of the nutrient medium, until Reach the end of the logarithmic growth phase of Haematococcus pluvialis, and obtain a large number of algae species;

[0034] Step 2, producing astaxanthin: Inoculate Haematococcus pluvialls into the production medium, and produce astaxanthin at a temperature of 24°C, wherein the light on Haematococcus pluvialls is continuously maintained during the cultivation process, and Ensure that the light intensity reaching the cell surface of Haematococcus pluvialis is 60 μmol m -2 ·s -1 Adding ethanol and sea crystals simultaneously in the production mediu...

Embodiment 3

[0037] Step 1, mass reproduction of algae species: Inoculate Haematococcus pluvialis into the nutrient medium at a temperature of 26°C and a continuous light intensity of 100 μmol·m -2 ·s -1And at the same time, continue to pass into the nutrient medium through filtering under the air condition of 2% carbon dioxide with a volume concentration of 2%, and its feed rate is to pass into 0.3L / min in every 1L of the nutrient medium, until Reach the end of the logarithmic growth phase of Haematococcus pluvialis, and obtain a large number of algae species;

[0038] Step 2. Production of astaxanthin: inoculate Haematococcus pluvialls into the production medium, and produce astaxanthin at a temperature of 26°C, wherein the light on Haematococcus pluvialls is continuously maintained during the cultivation process, and Ensure that the light intensity reaching the cell surface of Haematococcus pluvialis is 100 μmol m -2 ·s -1 Add ethanol and sea crystal simultaneously in the production ...

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Abstract

The invention discloses a method for producing astaxanthin by inducing Haematococcus pluvialis, which is characterized by comprising the following steps: in the vegetative growth phase, inoculating Haematococcus pluvialis in a nutrient culture medium, and culturing; continuously keeping irradiation on the Haematococcus pluvialis and ensuring that the intensity of illumination arriving at the Haematococcus pluvialis cell surface is 20-200 mu mol.m<-2>.s<-1>; in the induction stage, adding ethanol and seawater crystal into the production culture medium, wherein the ethanol added into the production culture medium accounts for 0.5-3 vol%, and the seawater crystal added into the production culture medium accounts for 0.5-1 wt%; and continuously introducing filtered air mixed with 1-3 vol% carbon dioxide into the production culture medium, wherein 0.2-0.4L / minute of the air is introduced to every 1L of production culture medium. The method can induce the Haematococcus pluvialis to quickly accumulate the astaxanthin, is simple and has the advantages of high yield and favorable effect.

Description

technical field [0001] The invention relates to the technical field of microorganism application, and mainly relates to a method for inducing Haematococcus pluvialis to produce astaxanthin. Background technique [0002] Astaxanthin (3,3'-dihydroxy-4,4'-diketo-β,β'-carotene, C40H52O4) is a carotenoid widely present in nature. Like other carotenoids, it has a long conjugated double bond, and the end of the double bond also contains unsaturated α-hydroxy ketones, which makes astaxanthin have stronger antioxidant activity than ordinary carotenoids, higher than β-carotene is more than 10 times that of vitamin E, 550 times that of vitamin E; secondly, astaxanthin is the only carotenoid that can penetrate the blood-brain barrier, so it plays an important role in anti-aging, anti-tumor, improving immunity, and preventing cardiovascular and cerebrovascular diseases. It has a significant effect and has the potential to be developed as an antitumor drug. Studies on the biological fun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P23/00C12R1/89
Inventor 温泽文陈方见刘智永陈树林侯余勇郑玉彬
Owner BIOALGO CY CO LTD
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