Method for extracting morroniside and loganin from Macrocarpium officinalis

A technology of Cornus officinalis and morroniside, which is applied in the technical field of separating morroniside and loganin by high-speed countercurrent chromatography, can solve the problems of easy structure change, large loss of active ingredients, and reduced extraction rate, etc. Reduce loss, good separation effect

Inactive Publication Date: 2012-05-30
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such as the patent "Preparation method and application of morroniside from Cornus officinalis extract" (application number is 200410014771.2), the method disclosed in this patent is to extract the medicinal material of Cornus officinalis with ethanol, concentrate under reduced pressure, add water to the concentrated solution, and absorb the supernatant with macropores Carry out column chromatography with resin or activated carbon, wash away impurities with water and dilute alcohol, and then elute with ethanol, collect the eluate, concentrate under reduced pressure and dry, and then separate through reverse-phase silica gel to obtain morroniside, the extract of Cornus officinalis in powder form. Method The impurities are eluted with water and dilute alcohol, and then the active ingredients are eluted w

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 1kg of dry Cornus officinalis and grind it, ultrasonically extract it with 6L 70% ethanol for 3 hours, extract 3 times, combine the extracts, concentrate without alcohol, add 1L water to disperse, add an equal volume of n-butanol to extract 4 times, concentrate the n-butanol layer to recover normal butanol to dryness to obtain crude glycosides, take 800ml n-butanol, 200ml ethanol and 1000ml water, mix them by ultrasonic vibration, transfer to a separatory funnel, let stand to separate layers, separate the upper and lower phases, the upper phase is the stationary phase, and the lower phase is mobile phase. The above-mentioned crude glycosides are dissolved in 350ml of the lower phase, and the upper phase is filled with a pump at a flow rate of 10ml / min to the chromatographic column, the head end of the column is connected to the injection valve, the speed controller is turned on, and the spiral tube rotates clockwise. When the rotational speed of the chromatograph w...

Embodiment 2

[0021] Take 1kg of dried Cornus officinalis and grind it, ultrasonically extract it with 8L 70% ethanol for 3 hours, extract twice, combine the extracts, concentrate without alcohol, add 800ml water to disperse, add an equal volume of n-butanol to extract three times, concentrate and recover the n-butanol layer n-butanol to dryness to obtain crude glycosides, take 700ml n-butanol, 400ml ethanol and 1000ml water, mix them by ultrasonic vibration, transfer to a separatory funnel, let stand to separate layers, separate the upper and lower phases, the upper phase is the stationary phase, and the lower phase for the mobile phase. The above crude glycosides were dissolved in 300ml of the lower phase, and the upper phase was filled with a pump at a flow rate of 10ml / min to the chromatographic column, the head end of the column was connected to the injection valve, the speed controller was turned on, and the spiral tube was rotated clockwise. When the rotational speed of the chromatog...

Embodiment 3

[0023] Take 2kg of dry Cornus officinalis and grind it, ultrasonically extract it with 15L 70% ethanol for 2 hours, extract 3 times, combine the extracts, concentrate without alcohol, add 1.5L water to disperse, add an equal volume of n-butanol to extract twice, and concentrate the n-butanol layer Recover n-butanol to dryness to obtain crude glycosides, take 1600ml n-butanol, 500ml ethanol and 1800ml water, mix them by ultrasonic vibration, transfer to a separatory funnel, let stand to separate layers, separate the upper and lower phases, the upper phase is the stationary phase, and the lower phase phase is the mobile phase. The above-mentioned crude glycosides were dissolved in 700ml of the lower phase, and the upper phase was filled with a pump at a flow rate of 12ml / min. The head end of the column was connected to the injection valve, and the speed controller was turned on to make the spiral tube rotate clockwise. When the rotational speed of the chromatograph was 950r / min,...

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Abstract

The present invention relates to a method for extracting morroniside and loganin from Macrocarpium officinalis. The method comprises ultrasonically extracting with 70% ethanol, extracting with n-butanol, concentrating an extractive solution and recovering n-butanol to near dryness to obtain crude morroniside and loganin, adopting an n-butanol-ethanol-water system as a two-phase solvent system, dissolving the crude morroniside and loganin in a bottom phase, separating by high-speed countercurrent chromatography, concentrating eluates under a reduced pressure to near dryness, respectively, crystallizing with n-butanol and ethyl acetate for 2-3 times, respectively, filtering out crystal, and drying to obtain the final products. The method has a simple process and low cost. The products have high purity.

Description

Technical field: [0001] The invention relates to a process for extracting morroniside and loganin from Cornus officinalis, in particular to a process for separating morroniside and loganin by high-speed countercurrent chromatography. Background technique: [0002] Cornus officinalis (Fructus Corni) is the dry pulp of Cornus officinalis Sieb.et Zucc. It is a kind of nourishing and precious medicinal material, which has the effects of nourishing the liver and kidney, astringent essence and detoxification. Studies in recent years have shown that Cornus officinalis has anti-hemorrhagic shock, inhibition of platelet aggregation, immune regulation, anti-inflammation, hypoglycemic, anti-oxidation, anti-cancer, and antibacterial effects. Cornus officinalis mainly contains iridoid glycosides, polysaccharides, organic acids, tannins and other components, with the highest content of morroniside and loganin. [0003] Since ancient times, there have been records in my country that Corn...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
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