Method for separating and extracting cannabidiol by high-speed countercurrent chromatography

A high-speed countercurrent chromatography, cannabidiol technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of no process parameters, inability to separate effects, and low CBD purity.

Inactive Publication Date: 2019-09-20
黑龙江康源生物科技有限公司
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  • Summary
  • Abstract
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  • Application Information

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Problems solved by technology

For example, in the extraction of CBD, if the parameters and materials are not selected correctly, the effect cannot be separated, or the purity of the isolated CBD is not high
[0011] At present, there is no good process parameter, so that high-speed countercurrent chromatography can purify c

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Step 1: Take industrial hemp flowers and leaves and dry them in the shade; dry the shade-dried industrial hemp flowers and leaves at 140°C for 0.4 hours to fully remove the water in the flowers and leaves, then crush them to more than 40 meshes, and extract them by percolation with 95% ethanol at room temperature , wherein the solid-liquid ratio of industrial hemp flower leaves and 95% ethanol is 1:30, then rotary evaporation, reclaiming extraction solvent, obtains the alcohol extract of industrial hemp flower leaves;

[0027] Step 2: Suspend the alcohol extract of industrial hemp flowers and leaves with water, then extract twice with n-butanol, ethyl acetate, and petroleum ether in sequence, combine the petroleum ether extraction parts obtained from the two extractions, and concentrate to remove petroleum ether Solvent, get sherwood oil extract; Wherein the consumption ratio of hemp leaf ethanol extract and water is 1:20; The volume consumption ratio of n-butanol, ethyl...

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PUM

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Abstract

The invention discloses a method for separating and extracting cannabidiol by high-speed countercurrent chromatography, belonging to the field of industrial hemp extraction. The method comprises the following steps: 1) drying and crushing industrial hemp flowers and leaves; 2) extracting the cannabidiol with methanol or ethanol to obtain a cannabidiol alcohol extract; 3) extracting with petroleum ether to obtain a petroleum ether phase, and removing the petroleum ether to obtain extract; 4) separating the extract by high-speed countercurrent chromatography, and collecting eluent; and 5) concentrating and crystallizing the eluent to obtain a cannabidiol product.

Description

technical field [0001] The invention relates to the field of industrial hemp, in particular to a method for separating and extracting cannabidiol by high-speed countercurrent chromatography. Background technique [0002] Cannabis sativa (Cannabis sativa L.subsp.sativa) is an annual herb with important medicinal value, belonging to the genus Cannabis subfamily of Moraceae. There are three main active ingredients in hemp plant extract (CBD, CBN, THC), among which THC is an addictive ingredient. The hallucinogenic effect of marijuana plants on humans is mainly determined by the concentration of THC, and varieties with THC<0.3% have no psychoactive effect. Therefore, the European Commission has formulated a unified standard that the THC content in dry cannabis products can not be higher than 0.3%, which is called "industrial hemp". [0003] At present, there are more than 80 kinds of cannabinoids isolated from cannabis, mainly including cannabidiol CBD, tetrahydrocannabinoi...

Claims

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Application Information

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IPC IPC(8): C07C37/68C07C37/70C07C37/72C07C37/82C07C37/84C07C39/23
CPCC07C37/004C07C2601/16C07C39/23
Inventor 屈晓宇邓小宽罗禹高平
Owner 黑龙江康源生物科技有限公司
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