Method for preparing high-purity taste-removing purple sweet potato pigment

Abstract

The invention discloses a method for preparing a high-purity taste-removing purple sweet potato pigment, and belongs to the field of food additives and agricultural product deep processing. The method for preparing the high-purity taste-removing purple sweet potato pigment comprises the steps that 1, pretreatment is conducted on purple sweet potato raw materials; 2, the purple sweet potato pigment is extracted from low-frequency microwave auxiliary acidified aqueous solution; 3, preliminary purification is conducted on macroporous resins; 4, high-speed counter-current chromatography separation and purification are conducted, and the taste-removing purple sweet potato pigment with concentration more than 95% is obtained. According to the method for preparing the high-purity taste-removing purple sweet potato pigment, the microwave auxiliary acidified aqueous solution is used for extraction, flocculants are added to flocculate mucoproteins, then macroporous resin static adsorption and dynamic elution are conducted, and the taste-removing and enrichment purification process is preliminarily accomplished, and the high-purity taste-removing purple sweet potato pigment is finally prepared through the high speed counter-current chromatography method in a separation mode. According to the method for preparing the high-purity taste-removing purple sweet potato pigment, technological processes are advanced, separation efficiency is high, recovery rate is high, product purity is more than 95%, preparation amount is large, an obtained product is free of peculiar smell, has few impurities and is good in solubility, and method for preparing the high-purity taste-removing purple sweet potato pigment is easy to popularize and use.

Description

technical field [0001] The invention relates to a method for preparing high-purity odorless purple sweet potato pigment by using an acidic aqueous solution as an extractant through preliminary purification of macroporous adsorption resin and separation and purification of high-speed countercurrent chromatography, belonging to the field of food additives and deep processing of agricultural products. Background technique [0002] Chemically synthesized pigments are bright in color and strong in coloring, but they are mainly aniline pigments. When added to food, some will form carcinogens in the body. With the in-depth research on the harmfulness of synthetic pigments to human health, and with the enhancement of people's psychological needs for advocating nature, returning to nature, and safety first, natural pigments have been valued and developed rapidly. It will become a major trend in the future that natural pigments replace synthetic pigments in food, medicine, cosmetics a...

AI Technical Summary

Problems solved by technology

Methods for extracting natural pigments reported in existing patent literature, such as patents "A Method for Continuous Countercurrent Ultrasonic Extraction of Purple Sweet Potato Anthocyanins" (Chinese Patent Application No. 201210038583.8), "Extraction Method of Sweet Potato Pigment" (Chinese Patent Application No. 02110033.0) , "Ethanol Extraction Method of Natural Red Pigment of Purple Sweet Potato" (Chinese Patent Application No. 200610005525.X), these methods all use organic solvent extraction method, which has certain disadvantages: (1) The consumption of organic solvent is large; (2) Organic solvent It is best to remove the organic solvent in the solution before loading the extract on the column, otherwise it will seriously affect the adsorption effect of the resin; if it is concentrated, the temperature should not be too high, so it must be concentrated under reduced pressure, which will inevitably increase the production cost; (3) Organic solvent extraction A large amount of organic solvents remain in the final potato residues, which cannot be recovered cleanly, so that the potato residues cannot be reused, resulting in a waste of resources; (4) Some alcohol-soluble proteins in sweet potatoes enter the organic solvent during extraction, and protein and other substances will be correspondingly increased during the concentration process After the resin is purified, the protein is directly discharged with the eluent, causing pollution to the environment

Both sulfuric acid and methanol are harmful to the human body. If the operation is not strict or other reasons in the production, it will cause harm to producers or consumers, and may even cause immeasurable losses to the enterprise.