Extraction method and and purification method of Sanghuang mushroom polysaccharide

A Phellinus polysaccharide and crude polysaccharide technology, which is applied in the digestive system, anti-toxins, anti-tumor drugs, etc., can solve the problems of low yield of Phellinus crude polysaccharide and low purity of Phellinus polysaccharide, achieve short purification time and improve product quality The effect of improving the purity and separation efficiency

Inactive Publication Date: 2008-12-17
SHANGHAI PUCHENG BIOTECHOLOGY
View PDF0 Cites 26 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] One of the technical problems to be solved in the present invention is to disclose a method for extracting Phellinus crude polysaccharides to overcome the technical defect of low yield of Phellinus crude polysaccharides in the prior art
[0010] The second tec

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Cut 1000g Phellinus Phellinus fruiting bodies into appropriate small pieces, put into a high-speed traditional Chinese medicine pulverizer and pulverize, and the pulverization fineness reaches 60 mesh. The pulverized Phellinus powder was mixed with 6L deionized water, and placed in an ultrasonic extractor for ultrasonic extraction. The ultrasonic temperature is 60°C, the ultrasonic time is 30min (2 seconds on, 3 seconds off), and the ultrasonic power is 1000W. Centrifuge at 5000rpm for 10min, and re-operate the obtained precipitate according to the above-mentioned ultrasonic extraction conditions. Centrifuge at 5000 rpm for 10 min, and combine the supernatant obtained after two extractions and centrifugation. Concentrate the extract to 1 / 3 of the original volume in a rotary evaporator, and add three times the volume of absolute ethanol to the concentrate. Centrifuge at 5000rpm for 10min, place the resulting precipitate into a dialysis bag with a membrane cut-off molec...

Embodiment 2

[0034] An aqueous two-phase system of 18% PEG1000 and 14% KPi (pH 6.8) was prepared, divided into an upper layer solution and a lower layer solution, the lower phase being the stationary phase, and the upper phase being the mobile phase. Weigh 200 mg of crude polysaccharide extract, and use mobile phase to prepare 20 ml of sample solution. After filling the entire HS-CCC helical column with the stationary phase, turn on the chromatograph, adjust the rotation speed to 600r / min, then introduce the mobile phase at a flow rate of 1ml / min, and inject the sample after the two phases reach equilibrium; and use a rotation speed of 600r / min , eluted with a mobile phase at a flow rate of 1ml / min, collected an eluent for every 6ml, combined the 26th to 30th eluents, and freeze-dried. The obtained product has a polysaccharide content of 76.7% and a protein content of 18.8%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention pertains to the field of extracting active ingredients of natural products and particularly relates to an extracting method and a purifying method of crude phellinus igniarius polysaccharides. The technical problems needed to be solved are to disclose a method for extracting the extract of crude phellinus igniarius polysaccharides and an efficient method for preparing phellinus igniarius polysaccharides through countercurrent chromatography. An ultrasonic extraction is adopted by the invention to extract crude phellinus igniarius polysaccharides from phellinus igniarius fruit bodies; the high-speed countercurrent chromatography is adopted to further purify the crude phellinus igniarius polysaccharides. The extracting method and the purifying method of the invention have high yield of crude phellinus igniarius polysaccharides, and can take up 6 percent of crude drugs; the purity of the further purified phellinus igniarius polysaccharides is greatly improved to be about 75 percent.

Description

technical field [0001] The invention belongs to the field of extracting effective components of natural products, and in particular relates to a method for extracting Phellinus crude polysaccharide and a method for purifying Phellinus polysaccharide. Background technique [0002] Phellinus linteus (Phellinus linteus), parasitic on poplar, willow, birch, oak, pine and other trees, is widely distributed and is a traditional Chinese medicine for oriental people (especially China, Korea and Japan, etc.). In recent years, Japan and South Korea have successively developed it. It is currently an internationally recognized medicinal fungus with very good efficacy in the field of biological anticancer. MeSima) has become a hotspot in the field of medicinal fungi research. It has been confirmed that the efficacy of Phellinus Phellinus mainly comes from polysaccharide from Phellinus linteus, and its pharmacological effects are as follows: [0003] 1. Anti-cancer: As early as 1968, Ja...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/00A61K36/06A61P1/16A61P39/06A61P29/00A61P31/04A61P35/00
Inventor 孙向军唐波许彦梅
Owner SHANGHAI PUCHENG BIOTECHOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap