Process for extracting xanthohumol from lupulus

A technology of xanthohumol and hops is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, separation/purification of carbonyl compounds, etc. The effect of high sample recovery, high purity and high yield

Inactive Publication Date: 2009-01-07
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The silica gel used in this method has irreversible adsorption to the relative sample, resulting in more loss of xanthohumol, and the yield of extracting and separating xanthohumol from hops is low

Method used

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  • Process for extracting xanthohumol from lupulus
  • Process for extracting xanthohumol from lupulus
  • Process for extracting xanthohumol from lupulus

Examples

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Embodiment 1

[0025] (1) Get the brewing particle hops of the brewery and pulverize into powder with a plant pulverizer;

[0026] (2) Use 1000ml of petroleum ether (boiling range 60-90°C) to reflux the above-mentioned 100g of crushed hops in a water bath to extract the fat-soluble components in the hops. The extraction temperature is 63°C, the extraction time is 45 minutes, and the extraction is repeated twice, and the oil is separated by filtration. ether solution to obtain defatted hops;

[0027] (3) 1000ml of 95% (v / v) ethanol was added to defatted hops to extract xanthohumol ultrasonically, the extraction temperature was 60°C, the extraction time was 40 minutes, the ultrasonic power was 200W, and the extraction was repeated twice. Filtrate, combine the extracts, and concentrate under reduced pressure to a thick paste; (after the thick paste is vacuum-dried, the xanthohumol content of the thick paste is 4.09% as determined by HPLC).

[0028] (4) Add 300ml of ethyl acetate to the above t...

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Abstract

The invention discloses a method for extracting xanthohumol from hops, which comprises the following steps: firstly pulverizing hops, adding petroleum ether to degrease fully, extracting with ethanol, filtering, concentrating the filtrate under reduced pressure to obtain a thick extract, distributing the thick extract with ethyl acetate / water, concentrating the ester phase under reduced pressure, vacuum-drying to obtain crude hop xanthohumol extract, and further separating and purifying the crude hop xanthohumol extract by two-step high-speed counter-current chromatography to finally obtain high-purity xanthohumol. The method overcomes the disadvantages of the conventional separation method such as the irreversible absorption effect of a sample, thereby improving the sample recovery rate. The yield of xanthohumol prepared from hops is up to 0.17% or more, and the purity of the prepared xanthohumol is up to 98% or more.

Description

technical field [0001] The invention relates to the extraction of xanthohumol, in particular to a method for extracting xanthohumol from hops. Background technique [0002] Hops (Humunus Lupulus L) are perennial herbaceous vines of the Moraceae Humulus genus, dioecious, unisexual flowers, female bulbs for short hops. Hops are mainly used as raw materials for beer brewing to impart bitterness, aroma and antiseptic effect to beer. The active ingredients are hop resin, hop oil and polyphenols. Xanthohumol (xanthohumol XN) is a flavonoid in hops, which is hop prenyl chalcone, and its chemical structure is as follows: [0003] [0004] In recent years, the research on xanthohumol has attracted much attention. It has been found that xanthohumol has many beneficial effects, such as anti-HIV, anti-cancer, anti-oxidation, anti-inflammation, estrogen, etc. Due to its important biological activity, it is urgently needed A large number of pure products are carried out in-depth rese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C49/84C07C49/835C07C45/78A61K36/60
Inventor 杨小兰毛立新郑海鹰杨磊胡仕屏罗正明
Owner SHANXI UNIV
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