Method for separating and preparing caffeic tannic acid from honeysuckle by utilizing high speed countercurrent chromatography

A technology of high-speed countercurrent chromatography and coffee tannin, which is applied in the field of plant separation to achieve the effect of high purity, less solvent consumption and large preparation volume

Inactive Publication Date: 2012-03-21
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the method for separating and preparing coffee tannin by high-speed countercurrent chromatography from honeysuckle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The honeysuckle is dried and crushed and passed through a 40-mesh sieve, then placed in an extraction tank and extracted with deionized water, and the weight ratio of honeysuckle to the deionized water is 1 : 8. The temperature of the deionized water is controlled at 60°C, and the extraction time is controlled at 2 hours. The extract formed by the deionized water + honeysuckle is filtered while it is hot and cooled to prepare the honeysuckle water extract and honeysuckle residue.

[0035] Put the prepared honeysuckle water into the first reaction vessel, then add absolute ethanol to the first reaction vessel until the ethanol concentration in the reaction vessel reaches 60% by volume, and place the honeysuckle water overnight + ethanol is filtered to obtain a filtrate, and then the filtrate is decompressed to recover ethanol and prepare a honeysuckle alcohol precipitation extract.

[0036] The prepared described Lonicera alcohol precipitation extract is packed in the s...

Embodiment 2

[0040] The honeysuckle is dried and crushed and passed through a 40-mesh sieve, then placed in an extraction tank and extracted with deionized water, and the weight ratio of honeysuckle to the deionized water is 1 : 9. The temperature of the deionized water is controlled at 75° C., and the extraction time is controlled at 1.5 hours. The extract formed by the deionized water + honeysuckle is filtered while it is hot and cooled to prepare honeysuckle water for extracting liquid and honeysuckle residue.

[0041] Put the prepared honeysuckle water into the first reaction vessel, then add absolute ethanol to the first reaction vessel until the ethanol concentration in the reaction vessel reaches 70% by volume, and place the honeysuckle water overnight + ethanol is filtered to obtain a filtrate, and then the filtrate is decompressed to recover ethanol and prepare a honeysuckle alcohol precipitation extract.

[0042] The prepared described Lonicera alcohol precipitation extract is pac...

Embodiment 3

[0046] The honeysuckle is dried and crushed and passed through a 40-mesh sieve, then placed in an extraction tank and extracted with deionized water, and the weight ratio of honeysuckle to the deionized water is 1 : 10. The temperature of the deionized water is controlled at 90° C., and the extraction time is controlled at 1 hour. The extract formed by the deionized water + honeysuckle is filtered while it is hot and cooled to prepare the honeysuckle water extract and honeysuckle residue.

[0047] Put the prepared honeysuckle water into the first reaction vessel, then add absolute ethanol to the first reaction vessel until the ethanol concentration in the reaction vessel reaches 80% by volume, and place the honeysuckle water overnight + ethanol is filtered to obtain a filtrate, and then the filtrate is decompressed to recover ethanol and prepare a honeysuckle alcohol precipitation extract.

[0048] The prepared described Lonicera alcohol precipitation extract is packed in the...

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PUM

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Abstract

The invention relates to a method for separating and preparing caffeic tannic acid from honeysuckle by utilizing a high speed countercurrent chromatography, comprising five processing steps of preparing water extract of the honeysuckle, preparing alcohol precipitation extract of the honeysuckle, extracting and preparing crude extract of the caffeic tannic acid, preparing a solvent system of stationary phase and mobile phase, separating and purifying by utilizing the high speed countercurrent chromatography. According to the invention, the crude extract of the caffeic tannic acid is extracted from the honeysuckle; and on the basis of confirming the best solvent system for separating the caffeic tannic acid, the caffeic tannic acid with purity up to more than 95% is finally prepared at a time through controlling separation parameters of the high speed countercurrent chromatography, such as rotating speed of a main engine, flow velocity of the mobile phase, temperature of a constant temperature circulator and the like. The method provided by the invention has the characteristics of simpleness and convenience for operation, mild process, good separation effect, high purity, safety and reliability.

Description

technical field [0001] The invention belongs to the technical field of plant separation, and in particular relates to a method for separating and preparing coffee tannin from honeysuckle by high-speed countercurrent chromatography. [0002] Background technique [0003] The 2005 edition of "Chinese Pharmacopoeia" stipulates that Lonicera japonica Thunb is the legal plant source of honeysuckle medicinal materials. [0004] Honeysuckle is a green natural product with the same source of medicine and food. Honeysuckle contains coffee tannin, and its chemical name is: 1,3,4,5-tetrahydroxycyclohexanecarboxylic acid-(3,4-dihydroxycinnamic acid Esters); belong to caffeoylquinic acid derivatives. [0005] Coffee tannin is considered to be the main active ingredient of antibacterial and detoxifying, anti-inflammatory and choleretic in many medicinal materials and Chinese patent medicines. The main biological activities of coffee tannins are reported to be: [0006] (1) It has inhi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48
Inventor 罗磊朱文学樊金玲李欣白喜婷关随霞李鑫玲郝英超
Owner HENAN UNIV OF SCI & TECH
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