Technique for preparing general flavone of Chinese globeflower with short petal some medicinal substances in high purity

A technology of total flavonoids and nasturtium lotus is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., which can solve the problems of environmental pollution, irreversible adsorption, time-consuming and laborious, and achieve high separation efficiency and easy popularization and use. , Overcome the effect of cumbersome operation

Active Publication Date: 2005-08-31
CHENGDE TIANYUAN PHARMACEUTICAL INDUSTRY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, at home and abroad, traditional separation and preparation methods such as column chromatography and recrystallization are used to separate effective monomers in plants, which is time-consuming and labor-intensive, and pollutes the environment [C.L Ky, M.Noirot, S.Hamon, J.Agric.Food. Chem.1997, 45:786; W.M.Yan, Acta Botanica Sinica, 1979, 20:54; J.J Liu, G.L.Zhao, H.Wang, X.H.Zhang, J.Cent.South.Univ.T.2002, 9:246] , and the immobilization used has some shortcomings such as irreversible adsorption to the sample

Method used

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  • Technique for preparing general flavone of Chinese globeflower with short petal some medicinal substances in high purity
  • Technique for preparing general flavone of Chinese globeflower with short petal some medicinal substances in high purity
  • Technique for preparing general flavone of Chinese globeflower with short petal some medicinal substances in high purity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Such as figure 1 The flow chart of the preparation of total flavonoids from Trollius chinensis is shown in

[0036] 1. Medicinal material extraction: Weigh 300g of dried nasturtium, extract twice with 10 times the amount of 60% ethanol under reflux, each time for 2 hours, filter, combine the filtrates, concentrate under reduced pressure (60°C) until there is no alcohol smell, and obtain the concentrated of the extract.

[0037] 2. Preparation of total flavonoids: take the concentrated extract, add water to suspend (the total volume is 900ml). Add it to a chromatographic column equipped with 500 g of pretreated 1300 macroporous adsorption resin, and absorb for 1.0 hour after loading the sample. Elute with 1200ml of water (discard). Then elute with 2000ml 30% medicinal ethanol, concentrate the ethanol eluted fraction under reduced pressure, and vacuum dry (60° C.) to obtain 16.7 g of total flavonoid powder, the extraction yield is 5.57%, and the total flavonoid purity ...

Embodiment 2

[0050] 1. Weigh 300g of dried nasturtium, extract twice with 10 times the amount of 50% ethanol under reflux, each time for 2 hours, filter, combine the filtrates, concentrate under reduced pressure (60°C) until there is no alcohol smell, and obtain the concentrated extract .

[0051] 2. Take the concentrated extract, add water to suspend, and the total volume is 900ml. Add it to a chromatographic column equipped with 500 g of pretreated AB-8 macroporous adsorption resin, and absorb for 1.0 hour after loading the sample. Elute with 1400ml of water (discard). Then elute with 2200ml 25% medicinal ethanol, concentrate the ethanol eluted fraction under reduced pressure, and vacuum dry (60° C.) to obtain 16.3 g of powder, the yield is 5.43%, and the purity of the total flavonoids is 62.2%.

[0052] 3. The methods, steps and results of the separation and purification, purity detection and structural identification of quercetin-neohesperidin, orientin and vitexin in total flavonoid...

Embodiment 3

[0054] 1. Weigh 300g of dried nasturtium, extract twice with 10 times the amount of 70% ethanol under reflux, each time for 2 hours, filter, combine the filtrates, concentrate under reduced pressure (60°C) until there is no alcohol smell, and obtain the concentrated extract .

[0055] 2. Take the concentrated extract, add water to suspend, and the total volume is 900ml. Add it to a chromatographic column equipped with 500 g of pretreated AB-8 macroporous adsorption resin, and absorb for 1.0 hour after loading the sample. Elute with 1400ml of water (discard). Then elute with 2000ml 35% medicinal ethanol, concentrate the ethanol eluted fraction under reduced pressure, and vacuum dry (60° C.) to obtain 18.6 g of total flavonoid powder, the yield is 6.20%, and the total flavonoid purity is 63.3%.

[0056] 3. The methods, steps and results of the separation and purification, purity detection and structural identification of quercetin-neohesperidin, orientin and vitexin in total f...

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Abstract

A process for preparing globeflower flavone, luxtexin, vitexin and quercetin-neohesperidoside from Chinese globeflower flower includes such steps as reflux extract in alcohol solution, adsorbing by macroreticular resin, cluting, concentrating, drying to obtain globeflower flavone, and separating and purifying, luxtexin, vitexin and quercetin-neohesperioside by HSCCC.

Description

technical field [0001] The present invention relates to a method for separating and purifying active ingredients of traditional Chinese medicines, namely, to provide a method for extracting total flavonoids from nasturtium brevipetalum and separating and purifying high-purity orientin, vitexin and quercetin-neohesperidoside from the total flavonoids Preparation Process. Background technique [0002] Nasturtium, also known as nasturtium, is the dried flower of Trollius ledebouri Reichb of the Ranunculaceae plant, mainly produced in Northeast China, North China and Inner Mongolia. Nasturtium was first seen in "Compendium of Materia Medica Supplements", and it has obvious inhibitory effects on Gram-positive cocci and Gram-negative bacilli, Streptococcus A, Catharella, Pseudomonas aeruginosa, Escherichia coli, and Shigella. Clinically, it is mainly used for the treatment of acute appendicitis, bacillary dysentery, upper respiratory tract infection, urinary tract infection, etc....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/00C07H1/08C07H17/07
Inventor 范国荣周欣彭金咏曲丽萍吴玉田柴逸峰
Owner CHENGDE TIANYUAN PHARMACEUTICAL INDUSTRY CO LTD
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