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Process for extracting buddleoside

An extraction process and a technology for cylindroside, which is applied in the field of cymenoside extraction technology, can solve problems such as no extraction of cymenoside, and achieve the effects of large preparation amount, simple extraction process and easy industrialization.

Inactive Publication Date: 2011-01-26
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no domestic literature on the extraction process of chrysanthemum glycosides, and no patent has been found on the extraction of chrysanthemum glycosides from wild chrysanthemum after patent searches.

Method used

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  • Process for extracting buddleoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 1kg of wild chrysanthemum residue after extracting volatile oil and put it into a multi-functional extraction tank, add 10 times the amount of 75% methanol, heat and reflux for 0.5h extraction, filter while hot, extract 3 times, combine the extracts, and put the extracts first in the operating pressure difference Under 0.04Mpa, through the hollow fiber microfiltration membrane with a molecular weight cut-off of 100,000, the permeate A was obtained, and the permeate A was passed through a hollow fiber ultrafiltration membrane with a molecular weight cut-off of 2,000 under the operating pressure difference of 1MPa to obtain the permeate B. The permeate B was passed through a hollow fiber nanofiltration membrane with a molecular weight cut-off of 500 under the operating pressure difference of 1.0Mpa to obtain a concentrated solution, the concentrated solution was coated with AB-8 macroporous resin, and eluted with 65% ethanol until TLC was used. Fraction, collect the e...

Embodiment 2

[0025] Take 1kg of wild chrysanthemum residue after extraction of volatile oil and put it into a multi-functional extraction tank, add 6 times the amount of methanol, heat and reflux for extraction for 1 hour, filter while hot, extract 4 times, combine the extracts, and first put the extract under the operating pressure difference of 0.08Mpa The permeate A was processed by a hollow fiber microfiltration membrane with a molecular weight cut-off of 150,000. The permeate A was passed through a hollow fiber ultrafiltration membrane with a molecular weight cut-off of 3,000 under an operating pressure difference of 1.3 MPa to obtain a permeate B. Liquid B was passed through a hollow fiber nanofiltration membrane with a molecular weight cut-off of 400 under an operating pressure difference of 0.9 Mpa, and the concentrated solution was collected. The concentrated solution was applied to S-8 macroporous resin, and the methanol was eluted to TLC to detect the fraction without stigma. Col...

Embodiment 3

[0027] Take 2 kg of wild chrysanthemum residue after extracting volatile oil and put it into a multifunctional extraction tank, add 12 times the amount of deionized water, heat and reflux for extraction for 1.5 hours, filter while hot, extract twice, combine the extract, and put the extract first in the operating pressure difference Under 0.15Mpa, the permeate A is processed by a hollow fiber microfiltration membrane with a molecular weight cut-off of 200,000, and the permeate A is passed through a hollow fiber ultrafiltration membrane with a molecular weight cut-off of 4,000 under the operating pressure difference of 2MPa to obtain permeate B. The permeate B was passed through a hollow fiber nanofiltration membrane with a molecular weight cut-off of 300 under the operating pressure difference of 1.2Mpa, and the concentrated solution was collected. The concentrated solution was coated with HPD-600 macroporous resin and eluted with 60% ethanol until TLC detected no smears. The g...

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Abstract

The invention relates to a process for extracting buddleoside, which comprises the following steps of: adding water or an organic solvent in an amount which is 5 to 12 times the mass of residue of wild chrysanthemum and extracting for 1 to 5 times, wherein the residue is obtained after volatile oil is extracted from the wild chrysanthemum and is used as a raw material; combining extract, filtering, filtering filtrate by using a filter membrane, and performing chromatographic separation and purification on the filtrate by using a packed column; and drying to obtain a product. Membrane separation technology is adopted for separation and purification, so that on the one hand, the purity of the buddleoside in the product is improved, and on the other hand, sample solution before column chromatography is purified, damage to fillers is relieved, and the life cycle of the column chromatography fillers is prolonged; in addition, nanofiltration technology is applied, so that the sample solution before the column chromatography does not need to be concentrated and can be directly loaded, and the process is simplified.

Description

Technical field: [0001] The invention belongs to the technical field of natural medicine extraction, and in particular relates to an extraction process of mongolin. Background technique: [0002] Buddleoside [0003] Molecular formula: C28H32O14; Molecular weight: 592.5468; CAS number: 480-36-4; [0004] Molecular structural formula: [0005] [0006] Wild chrysanthemum is the flower of wild chrysanthemum and rock fragrant chrysanthemum, which has the effect of clearing away heat and detoxifying, soothing wind and calming liver. Indications for boils, carbuncle, erysipelas, eczema, dermatitis, wind-heat cold, sore throat, hypertension. Wild chrysanthemum water has obvious inhibitory effect on rat platelet aggregation induced by ADP, Staphylococcus aureus, and rabbit muscle collagen in test tube, and it is enhanced with the increase of dose; on Staphylococcus aureus, Escherichia coli, dysentery Bacillus and Pseudomonas aeruginosa have strong antibacterial effects; intr...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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