Method for separating and purifying mangiferin from aquilaria sinensis leaves

Abstract

The invention discloses a method for separating and purifying mangiferin from aquilaria sinensis leaves. The method is used for solving the problems such as low purity, low yield and complex operation of the mangiferin separated by virtue of the existing process. The method comprises the following step: quickly preparing the high-purity mangiferin by use of the aquilaria sinensis leaves used as raw materials by virtue of adopting a method of combining normal phase column chromatography with high-speed counter-current chromatography. The method has the advantages of great preparation amount, high efficiency, quickness, low cost, simplicity in operation, high product quality and no pollution; and moreover, a solvent can be recycled, the solvent cost and the solvent treatment cost can be lowered, and the method can be widely applied to industrial production.

Description

【Technical field】 [0001] The invention belongs to the field of extraction, separation and purification of natural compounds, and in particular relates to a method for rapidly preparing high-purity compounds by using column chromatography combined with HSCCC technology. 【Background technique】 [0002] Aquilaria sinensis, also known as soil agarwood, agarwood tree, Guanxiang, tooth incense, daughter incense, etc., is a plant of the genus Aquilaria in the family Daphneaceae, an evergreen tree, and is a unique national second-level protected plant in China. In Guangdong, Hainan, It is planted in Guangxi, Fujian and other places, and it is a rare and precious medicinal material commonly used in traditional Chinese medicine. The heartwood of Baimuxiang is pungent, bitter, and slightly warm. It has the effects of lowering Qi, warming the kidneys, and relieving pain. The resin-containing heartwood (commonly known as agarwood) of the tree body can be used as a raw material for spice...

AI Technical Summary

Problems solved by technology

[0010] 1. The purity of mangiferin obtained from most processes is low, or there is no exact guarantee of purity;

[0011] 2. Part of the process is complicated to operate, and a large amount of solvent is required for elution, which is easy to cause harm to the operator, and the cost of the solvent is high;

[0012] 3. The preparation cycle of most processes is long, the recovery rate is low, and the economic benefit is low;

[0013] 4. Mangiferin is unstable under acidic or alkaline conditions. If acid extraction or alkaline extraction is used, most mangiferin may be denatured and inactivated during the preparation process;

[0014] 5. Mango leaves are mostly used as raw materials, which are prone to excessive pesticide residues and are unsafe;

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