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Method of preparing Rhodiola sacra glycoside

A technology of salidroside and extract, which is applied in the field of medicine, can solve the problems of serious root rot of seedlings, difficulty in large-scale production, and occupation of a large area of ​​cultivated land, etc., and achieves the effects of easy acquisition, easy recycling, and high safety

Inactive Publication Date: 2006-06-14
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to protect this precious plant, artificial cultivation of Rhodiola plants has been attempted in recent years, but the content of salidroside under artificial cultivation conditions is low (less than 0.2%), the root rot of seedlings is serious, and it takes a large The area of ​​cultivated land limits the promotion of this technology
Although large-scale cell culture has achieved good results, the cost of culture is high and it is difficult to carry out large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: After 1 kg of Ligustrum lucidum is crushed, add 3 kg of 80% ethanol, stir evenly and fully mix, soak for 48 hours, reclaim the filtrate, extract three times, combine the filtrate, recycle the ethanol under reduced pressure to obtain the salidroside extract; The extract was adsorbed by weak polarity AB-8 macroporous adsorption resin for 30min, washed with pure water until the Molish reaction was negative, then eluted with 2 times the amount of 30% ethanol, the eluate was recovered and concentrated by ethanol, and then Weakly polar AB-8 macroporous adsorption resin was adsorbed for 30 minutes, washed with pure water until the Molish reaction was negative, and then eluted with 2 times the amount of 30% ethanol. The eluent was recovered from ethanol and concentrated, and dried in vacuum to obtain Salidroside crude product 3.065g. The prepared crude salidroside was further purified by a polyamide column and detected under the following chromatographic condition...

Embodiment 2

[0014] Embodiment 2: After 1 kg of Ligustrum lucidum was crushed, 3 kg of 70% ethanol was added, stirred evenly and fully mixed, soaked for 72 hours, the filtrate was recovered, extracted twice, the filtrate was combined, and the ethanol was recovered under reduced pressure to obtain a salidroside extract; The extract was adsorbed by weakly polar AB-8 macroporous adsorption resin for 30 minutes, washed with pure water until the Molish reaction was negative, and then eluted with 2 times the amount of 35% ethanol, and the eluate was recovered and concentrated by ethanol, and then Adsorbed by weakly polar AB-8 macroporous adsorption resin for 30 minutes, rinsed with pure water until the Molish reaction was negative, then eluted with 2 times the amount of 35% ethanol, the eluate was recovered from ethanol and concentrated, and dried in vacuum to obtain Salidroside crude product 2.988g. After the prepared crude salidroside was further purified by a polyamide column, the content of ...

Embodiment 3

[0015] Embodiment 3: After 1kg of Ligustrum lucidum is pulverized, add 4kg of 60% ethanol, stir evenly and fully mix, soak for 72 hours, reclaim the filtrate, extract three times, combine the filtrate, recycle the ethanol under reduced pressure to obtain the salidroside extract; The extract was absorbed by weak polarity AB-8 macroporous adsorption resin for 30min, washed with pure water until the Molish reaction was negative, and then eluted with 2 times the amount of 35% ethanol, the eluate was recovered and concentrated by ethanol, and then Weakly polar AB-8 macroporous adsorption resin was adsorbed for 30 minutes, washed with pure water until the Molish reaction was negative, and then eluted with 2 times the amount of 35% ethanol. The eluate was recovered from ethanol and concentrated, and it was red after vacuum drying. Crude sedroside 3.012g. After the prepared crude salidroside was further purified by a polyamide column, the content of salidroside in the crude salidrosid...

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Abstract

The present invention relates to a method for preparing rhodiola glucoside crude product by using glassy privet fruit as raw material and adopting macroporous adsorption resin purification technique. Said method includes the following steps: using 60%-80% ethyl alcohol to soak broken glossy privet fruit to obtain rhodiola glucoside extract, then making the obtained rhodiola glucoside extract undergo the processes of low-polar AB-8 type macroporous resin adsorption, washing with pure clean water, 25-35% ethyl alcohol elution, recovering ethyl alcohol and concentration so as to obtain the rhodiola glucoside crude product.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to a preparation method for extracting salidroside from Ligustrum lucidum. Background technique [0002] Salidroside, English name salidroside, chemical name "p-hydroxyphenethyl-β-D-glucopyranoside (p-hydroxyphenethyl-β-D-glucoside)", systematic name: 1-(4-hydroxy)benzene Ethyl-β-D-glucopyranoside is an active compound extracted from medicinal plants. Salidroside has the functions of enhancing learning and memory, improving cardiovascular and cerebrovascular systems, anti-fatigue, hypoxia resistance, and anti-aging , enhancing immunity, anti-virus and protecting organs, it is a very promising environmental adaption drug (Qu Zhiwei. Research progress of Rhodiola, Chinese Journal of Practical Traditional Chinese and Western Medicine, Volume 18, No. 7, 2005 : 1063-1065). At present, drugs developed using this compound are mainly based on natural Rhodiol...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H1/08
Inventor 石力夫王丹胡晋红
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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