32 results about "Rosavin" patented technology

The invention provides a rhodiola crenulata extract which is characterized in that: in the extract, the weight percentage of rhodioloside is not less than 10%, the weight percentage of rhodiola polysaccharide is not less than 30%, and the weight percentage of rhodiola polyphenol is not less than 34.0%; and the content of rosavin is 0%. The invention also provides a preparation method of the extract. The rhodiola crenulata provided by the invention has the advantages of exact drug effect, stable quality and strong controllability.

The invention discloses a Rhodida plant callus and suspension cell granule production method, comprising: (1) constructing a Rhodida plant aseptic culture system to culture and obtain aseptic Rhodida plants with roots, stems and leaves; (2) inoculating the roots, stems or leaves of the Rhodida plants into a callus induction culture medium to carry out callus induction culture and successive transfer culture to obtain good suspension cell granules originated from different organs of the Rhodida plants. The good suspension cell line originated from different organs of the Rhodida plants has the characteristics of fast growing speed, good dispersibility, high content of secondary metabolites (wherein the content scope of Salisorosides Rosavin and tyrosol are respectively 1-3% and 0.5-0.7% of the dry weight of the suspension cells) and the like, is very stable in successive transfer culture and degradation phenomenon does not exist.

The invention discloses a method for synchronously separating and preparing salidroside and rosavin from rhodiola rosea, comprising the following steps: (1) taking the rhodiola rosea as a raw material, and extracting by using analcohol aqueous solution containing a deep-eutectic solvent, wherein the deep-eutectic solvent has the effects of emulsifying, wetting, being assistant in dissolving and the like, and is beneficial for the extraction of the salidroside and the rosavin; (2) preparing a salidrosidecrude extracting solution and a rosavin crude extracting solution respectively from the extract by combining a mixed macroporous resinexpanded bed technology and a gradient elution technology; (3) preparing a salidrosideextract and a rosavin extract from the salidrosidecrude extracting solution and the rosavin crude extracting solution respectively by using the mixed macroporous resinexpanded bed technology again. Through HPLC content testing, the content of the salidroside in the salidroside extract is 40 percent to 80 percent, and the recovery is 60 percent to 88 percent; the content of the rosavin in the rosavinextract is 30 percent to 70 percent, and the recovery is 65 percent to 85 percent.

The invention relates to a process for preparing a rhodiola rosea extract with a high antioxidant value using the ultrafiltration method. The process comprises the following steps: (1) drying and crushing rhodiola rosea material to obtain crude powder, extracting with an ethanol solution, and then filtering and concentrating to obtain a concentrate; (2) adsorbing the concentrate via macroporous adsorbent resin, preparing elution solvents, eluting the resin with the solvents respectively, and collecting the eluates; (3) performing step-by-step membrane filtration on the eluates to obtain a target solution; (4) concentrating the target solution and then drying by the freeze-drying method, and producing the rhodiola rosea extract with a high antioxidant value. With adoption of the combination of the membrane filtration method and the freeze-drying method, a high content of rhodiola rosea extract with a high antioxidant value is obtained, and the rosavin content of the rhodiola rosea extract obtained by the method is 5-25%, the salidroside content is 5-20%, and the antioxidant value is 1000-2000 mumole TE/g.

The invention discloses composite anti-fatigue beverage containing plant extracts. The beverage disclosed by the invention consists of salidroside, rhodiola rosavin, astragalus saponin, hawthorn flavone, caffeine, taurine, inositol, nicotinamide, lysine, vitamins, citric acid, cane sugar, juice and water according to a reasonable ratio. The anti-fatigue beverage disclosed by the invention has the effects of resisting fatigue and improving the immunity.

The invention relates to a synthesis method for Beta-(E)-cinnamic alcohol group-O-[6'-O- Alpha-L-arabopyranose group]-D-glucose pyranoside (Rosavin). The method comprises: carrying out the glycoside reaction on Cinnamic alcohol and 2,3,4-tri-O-benzoyl-[6-O-(2',3',4'-tri-O-acetyl)-L-furan arabopyranose group ]-D-furan glucose trichloroacetimidate, and then removing a protective group, so as to obtain the Beta-(E)-cinnamic alcohol group-O-[6'-O- Alpha-L-arabopyranose group]-D-glucose pyranoside. The method has easily obtained raw materials, moderate reaction conditions and high product purity. The method is not reported in a document. Therefore, the synthesis method is a novel method for artificially synthesizing Rosavin.

The invention discloses a Chinese medicinal composition for resisting anoxia and fatigue and a preparation method thereof. The Chinese medicinal composition is prepared by adding excipients such as a disintegrant, a flavoring agent and the like into rhodiola rosea and borneol which are taken as raw materials. The content of rosavin and salidroside contained in a rhodiola rosea extract obtained by the method is improved; and the process is simple. Pharmacodynamic experiment results of the Chinese medicinal composition show that the medicine has remarkable effects of resisting anoxia and fatigue. The Chinese medicinal composition and the preparation method have the advantages that: the formula is simple, the preparation process is simple, and the medicine is convenient to use and has no toxic or side effect.

The invention discloses a method for efficiently extracting salidroside and rosavin from rhodiola rosea. The method comprises the following steps of: selecting rhodiola rosea roots, drying, and rapidly air-cooling; crushing the dried rhodiola rosea roots, and screening into the granularity of 40 meshes; adding 2% of hexadecyl trimethyl quaternary ammonium bromide and 80% alcohol solution of 1% of potassium acetate in an amount of 10 times of the weight of the rhodiola rosea into the obtained rhodiola rosea powder, performing dynamic countercurrent extraction at a temperature of 25 DEG C so as to obtain an extracting solution; performing vacuum concentration, concentrating into an extract proportion of 1.15-1.20; adding acidified pure water in an amount of 7 times of the weight into the obtained extract, fully stirring, precipitating in a refrigerated storage and staying overnight, and separating the precipitate; and performing spray drying, thereby obtaining the product. The production method provided by the invention is a one-step extraction method, the dynamic countercurrent extraction is adopted, extraction, separation and purification are simultaneously performed, the method is high in extraction speed, high in efficiency, low in energy consumption, simple in process and low in cost, and the product recovery rate is obviously improved.

The invention discloses a method for preparing rosavin from rose rhodiola rosea. The method comprises the following steps: pretreating raw materials; extracting; filtering; passing macroporous resin primarily; concentrating; passing resin again; separating membrane; spraying and drying, and the like. Compared with other methods, two times of column chromatography methods and the membrane method are adopted, so that the method disclosed by the invention is more suitable for industrial production. The product obtained by the method is high in safety, purity, quality and yield.

The invention discloses an anti-stress compound preparation, which consists of 30 weight parts of Chinese medicinal composition and 0.65 weight part of nutrient composition, wherein the Chinese medicinal composition comprises the following components in part by weight: 5 to 40 parts of 0.8 percent eleutheroside, 5 to 40 parts of 2 percent salisorosides rosavin, 3 to 20 parts of spina date seed, 10 to 70 parts of grassleaf sweelflag rhizome, 5 to 50 parts of tribulus terrestris L. and 5 to 40 parts of astragalus; and the nutrient composition comprises the following components in part by weight: 100 to 300 parts of tyrosine, 3 to 20 parts of vitamin C, 40 to 100 parts of vitamin B2, 100 to 220 parts of vitamin B1, 40 to 100 parts of vitamin B6, 100 to 220 parts of vitamin PP, 2 to 12 parts of folic acid, 40 to 120 parts of ferrous lactate and 200 to 800 vitamin E powder containing 30 percent of VE. Capsules of the invention are proved by clinical application to have the effects of supporting right, securing the root and enhancing the immunity of organisms, are applied to people with relatively higher life stress and work stress in the modern society, and can enhance the endurance of the organisms, reduce the stress-resisting stress levels of the organisms and improve the anti-stress capability of the organisms.

A cosmetic composition comprising a UV-protective amount of at least one rosavin, preferably present in an extract of Rhodiola rosea in a cosmetically acceptable vehicle, and methods of use thereof, including preventing or reducing the signs of photoaging.

The present invention provides a synthetic method of rhodiola rosea active ingredient rosavin or rosarin. A convergent synthetic strategy is adopted, rosin is used as a starting material and modifiedinto sugar acceptors through protection and deprotection strategies, then sugar acceptors and sugar donors formed by arabinopyranose and arabinyl furanose in presence of an accelerator quickly and efficiently conduct synthesis of rosavin and rosarin. The related synthetic method is cheap and easy-to-obtain in raw materials and mild in reaction conditions, the obtained product is high in purity, and compared with currently reported linear synthesis strategy, the method has very good industrialization prospects.

The invention discloses a preparation method of high-purity Rosavin. The preparation method comprises the following steps of: carrying out reflux extraction on the roots and the stems of herba rhodiolae by using an ethanol solution, respectively extracting by using petroleum ether and ethyl acetate, and concentrating extract liquor to obtain a Rosavin crude extract; carrying out separation and enrichment on the Rosavin crude extract by using macroporous adsorption resin, and eluting by using ethanol to obtain a Rosavin eluate; separating the Rosavin eluate by using a high-speed counter-current chromatography separation technology to obtain a Rosavin crude product; purifying the Rosavin crude product by using preparative high performance liquid chromatography to obtain a Rosavin refined product; and drying the Rosavin refined product in vacuum to obtain the high-purity Rosavin. The purity of the separated and extracted Rosavin is high, the purity is larger than 98.5%, and the obtained Rosavin can be used as a standard sample of the Rosavin.

The invention discloses a method for simultaneously detecting multiple components in rhodiola rosea by HPLC, which comprises the following steps: by using methanol as a solvent, extracting from crushed rhodiola rosea raw materials to obtain a test solution; respectively preparing a salidroside standard substance solution, a rosavin standard substance solution, a rosavin standard substance solutionand a cheek complexing standard substance solution, fixing the volumes of the solutions in a volumetric flask by using methanol, respectively taking the same amount of single standard substances, performing mixing to prepare a mixed standard substance, and filtering the mixed standard substance by using 0.4-0.5 [mu]l of an organic filter membrane; respectively carrying out quantitative analysis on the mixed standard solution and the test solution which pass through the organic filter membrane at dual wavelengths by utilizing HPLC, wherein the column temperature is 26-30 DEG C, the sample sizeis 10[mu]l, the detection wavelength is set to be 276 nm from 0 min to 8 min, and is set to be 255 nm from 8 min to 20 min; and S4, according to an HPLC analysis result, obtaining a spectrum of the test solution and a mixed standard spectrum, and carrying out comparative analysis. The invention provides the method for simultaneously detecting the contents of salidroside, rosavin, rosavin and cheek in rhodiola rosea, and the method has the characteristics of simple operation, less experiment times, high efficiency, accuracy and wide applicability.

The invention discloses a method for simultaneously extracting salidroside, rosavin and polysaccharide from rhodiola rosea. The method comprises the following steps of performing ethanol reflux extraction on a rhodiola rosea raw material, separating residues and filtrate, and concentrating the filtrate to obtain a crude extract containing the salidroside and the rosavin; performing water extraction, filtration, concentration and alcohol precipitation on residues to obtain a polysaccharide crude extract; passing the crude extract containing the salidroside and the rosavin through a non-polar macroporous resin column, correspondingly eluting with water and ethanol with different concentrations, and correspondigly collecting eluents to obtain a polysaccharide crude product, a salidroside crude product and a rosavin crude product; combining the polysaccharide crude extract and the eluent polysaccharide crude product, and removing protein and pigment to obtain a rhodiola rosea polysaccharide extract; and repeatedly recrystallizing the salidroside crude product and the rosavin crude product through a polyamide column and an organic solvent respectively to obtain salidroside and rosavin pure products. According to the method, the salidroside, the rosavin and the polysaccharide can be efficiently and simultaneously extracted.

The invention provides sea salt and rhodiolarosea extract containing plant salt. The sea salt and rhodiolarosea extract containing plant salt comprises sea salt and a rhodiolarosea extract. In the sea salt and rhodiolarosea extract containing plant salt, the total content of rosavin is 0.3%-2%, and the content of rhodioloside is 0.25%-1.5%. The invention also provides a method for preparing the plant salt by using a preheating enrichment crystallization process. The method comprises the step of removing heavy metals with brine; the step of removing heavy metals with brine includes the step of removing the heavy metals with an aeration method; in the step of removing the heavy metals with the aeration method, the brine is aerated by using ozone, and the aeration time lasts for 20-40 min. By using the special process provided by the invention, a prepared bio-adsorbent is enabled to have good removal effects on heavy metal ions, and the removal effect of the bio-adsorbent on lead ions is 10%.