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Method for simultaneously detecting multiple components in rhodiola rosea by HPLC

A Rhodiola and methanol technology, which is applied in measurement devices, material separation, analysis of materials, etc., can solve the problems of low reliability of detection results, large differences in components of Rhodiola, and complex detection process, and achieve high reliability. , the number of experiments is small, the effect of simple operation

Pending Publication Date: 2020-09-25
太阳树(厦门)生物工程有限公司
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Problems solved by technology

[0003] Due to the wide range of sources of Rhodiola plants in the market, the commonly used varieties in different regions are also different, and the components of Rhodiola from different sources are quite different. The existing methods for detecting multiple components in Rhodiola have a single detection component and the detection process It is complicated and the reliability of the detection results is not high, so it is necessary to develop a method that can quickly and accurately detect multiple components in Rhodiola

Method used

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  • Method for simultaneously detecting multiple components in rhodiola rosea by HPLC
  • Method for simultaneously detecting multiple components in rhodiola rosea by HPLC
  • Method for simultaneously detecting multiple components in rhodiola rosea by HPLC

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0022] A method for simultaneous detection of multiple components in Rhodiola Rosea by HPLC, comprising the steps of:

[0023] 1. The Rhodiola rosea raw material was crushed, and after accurate weighing, it was ultrasonically extracted with 60% methanol for 30 minutes, and then the ultrasonic liquid was transferred to a volumetric flask and fixed to volume with 60% methanol to obtain the test solution.

[0024] 2. Chromatographic conditions:

[0025] The chromatographic column is: ZORBAX-SB-Aq 5um 4.6×250mm.

[0026] The mobile phase is: methanol, 0.4% acetic acid, the initial ratio is 60:40, the methanol is increased from 60% to 85% in 0-8min, the 0.4% acetic acid is decreased from 40% to 15%, and the methanol is increased from 85% to 85% in 8-20min 95%, 0.4% phosphoric acid from 15% to 5%, 20-22min, methanol from 95% to 60%, 0.4% acetic aci...

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Abstract

The invention discloses a method for simultaneously detecting multiple components in rhodiola rosea by HPLC, which comprises the following steps: by using methanol as a solvent, extracting from crushed rhodiola rosea raw materials to obtain a test solution; respectively preparing a salidroside standard substance solution, a rosavin standard substance solution, a rosavin standard substance solutionand a cheek complexing standard substance solution, fixing the volumes of the solutions in a volumetric flask by using methanol, respectively taking the same amount of single standard substances, performing mixing to prepare a mixed standard substance, and filtering the mixed standard substance by using 0.4-0.5 [mu]l of an organic filter membrane; respectively carrying out quantitative analysis on the mixed standard solution and the test solution which pass through the organic filter membrane at dual wavelengths by utilizing HPLC, wherein the column temperature is 26-30 DEG C, the sample sizeis 10[mu]l, the detection wavelength is set to be 276 nm from 0 min to 8 min, and is set to be 255 nm from 8 min to 20 min; and S4, according to an HPLC analysis result, obtaining a spectrum of the test solution and a mixed standard spectrum, and carrying out comparative analysis. The invention provides the method for simultaneously detecting the contents of salidroside, rosavin, rosavin and cheek in rhodiola rosea, and the method has the characteristics of simple operation, less experiment times, high efficiency, accuracy and wide applicability.

Description

technical field [0001] The invention relates to the detection field of traditional Chinese medicinal materials, in particular to a method for simultaneously detecting multiple components in Rhodiola rosea by HPLC. Background technique [0002] Rhodiola rosea, alias: Rhodiola rosea, is a perennial herb, 10-20cm high, with thick, conical, fleshy, brownish-yellow roots, with many fibrous roots at the root neck, short, strong, cylindrical, covered by many Scale-like leaves arranged in tiles. It grows in the alpine and pollution-free zone at an altitude of 1800-2500 meters, and its growth environment is harsh, so it has strong vitality and special adaptability. It can be used as medicine, can invigorate qi and clear the lungs, improve intelligence and nourish the heart, and is a traditional Chinese medicine with a wide range of effects. It also has a great cosmetic effect and can be used as a skin care product or edible. [0003] Due to the wide range of sources of Rhodiola pl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 陈义刘裕陈强
Owner 太阳树(厦门)生物工程有限公司
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