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Method for simultaneously extracting salidroside, rosavin and polysaccharide from rhodiola rosea

A technology of salidroside and rhodiola, which is applied in the field of extraction of rhodiola, can solve the problems of high viscosity of deep eutectic solvent, large one-time investment, difficult recovery, etc., to reduce steps, save cost, and easy to obtain Effect

Active Publication Date: 2022-01-21
HUNAN NUSTREETCARAX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the above-mentioned method can obtain salidroside and luosaiwei extract, there are following defects: 1) at first added tetraethylammonium chloride in the extract , glucose and other substances, due to the high viscosity of the deep eutectic solvent, it is difficult to recycle, and because the solvent residue has certain safety hazards, it increases the cost to a certain extent; 2) Secondly, the macroporous resin expanded bed technology is different from the traditional fluid flow chemical bed requires special adsorbents and equipment, which requires the transformation of the original equipment of the factory, and the one-time investment is relatively large; utilize resource

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] 1) Take the dried Rhodiola rosea that has been removed from impurities, slice it, crush it through a 30-mesh sieve, weigh 50g, add 50% ethanol that is 5 times the weight of Rhodiola rosea powder, extract at 80°C for 2 hours, filter, add 4 times, 3 times the weight of Rhodiola rosea powder was extracted with 50% ethanol for 1.5 h, filtered, the filtrate and residue were separated, and all filtrates were combined.

[0071] The filtrate passed through a 24-mesh activated carbon column at a flow rate of 1 BV / h, and the column temperature of the activated carbon column was maintained at 60°C. The effluent was collected and concentrated until it had no alcohol smell, and an extract containing salidroside and rosevin was obtained.

[0072] 2) Take the residue from step 1), add water 4 times the weight of Rhodiola rosea powder, reflux extraction at 80°C for 3 hours, centrifuge to obtain the extract, concentrate to 1 / 4 of the original volume, add absolute ethanol to make the etha...

Embodiment 2

[0081] 1) Take the dried Rhodiola rosea that has been removed from impurities, slice it, crush it through a 60-mesh sieve, weigh 5kg, add 70% ethanol that is 8 times the weight of Rhodiola rosea powder, extract at 60°C for 1 hour, filter, and add 6 times, 4 times the weight of Rhodiola rosea powder was extracted with 7% ethanol for 0.5h, filtered, the filtrate and residue were separated, and the filtrates were combined three times.

[0082] The filtrate passed through a 100-mesh activated carbon column at a flow rate of 2BV / h, and the column temperature of the activated carbon column was maintained at 80°C. The effluent was collected and concentrated until it had no alcohol smell, and an extract containing salidroside and rosevin was obtained.

[0083] 2) Take the residue from step 1), add water 6 times the weight of Rhodiola rosea powder, reflux extraction at 90°C for 1 hour, centrifuge to obtain the extract, concentrate to 1 / 4 of the original volume, add absolute ethanol to m...

Embodiment 3

[0092] 1) Take the dried Rhodiola rosea after removal of impurities, slice it, crush it through a 50-mesh sieve, weigh 500kg, add 10 times the weight of Rhodiola rosea powder 60% ethanol, extract at 70°C for 1.5h, filter, and add 8 times the filter residue respectively , 6 times the weight of Rhodiola rosea powder with 60% ethanol to extract for 1 h, filter, separate the filtrate and residue, and combine the filtrates 3 times.

[0093] The filtrate passed through a 50-mesh activated carbon column at a flow rate of 3BV / h. The column temperature of the activated carbon column was kept at 70°C. The effluent was collected and concentrated until it had no alcohol smell, and an extract containing salidroside and rosevin was obtained.

[0094] 2) Take the residue from step 1), add water 5 times the weight of Rhodiola rosea powder, reflux extraction at 90°C for 1 hour, centrifuge to obtain the extract, concentrate to 1 / 4 of the original volume, add absolute ethanol to make the ethanol ...

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Abstract

The invention discloses a method for simultaneously extracting salidroside, rosavin and polysaccharide from rhodiola rosea. The method comprises the following steps of performing ethanol reflux extraction on a rhodiola rosea raw material, separating residues and filtrate, and concentrating the filtrate to obtain a crude extract containing the salidroside and the rosavin; performing water extraction, filtration, concentration and alcohol precipitation on residues to obtain a polysaccharide crude extract; passing the crude extract containing the salidroside and the rosavin through a non-polar macroporous resin column, correspondingly eluting with water and ethanol with different concentrations, and correspondigly collecting eluents to obtain a polysaccharide crude product, a salidroside crude product and a rosavin crude product; combining the polysaccharide crude extract and the eluent polysaccharide crude product, and removing protein and pigment to obtain a rhodiola rosea polysaccharide extract; and repeatedly recrystallizing the salidroside crude product and the rosavin crude product through a polyamide column and an organic solvent respectively to obtain salidroside and rosavin pure products. According to the method, the salidroside, the rosavin and the polysaccharide can be efficiently and simultaneously extracted.

Description

technical field [0001] The invention relates to the extraction of rhodiola components, in particular to a method for simultaneously extracting salidroside, rosevi and polysaccharide from rhodiola. Background technique [0002] Rhodiola rosea is a perennial herb of the Rhodiola family Rhodiola family. It is usually used as medicine with dried roots and rhizomes. Rhodiola is a traditional Chinese medicinal material in China and one of the local medicinal materials in Northeast my country. Due to the lack of wild resources of medicinal materials, it is a rare medicinal plant and is known as "snowy ginseng". Therefore, artificially cultivated rhodiola rosea is the main source of resources on the market. In view of the current serious environmental pollution, heavy metals may be enriched during the cultivation process. In addition, harmful elements may be brought into the process of processing, storage and production, and the raw materials are rich in heavy metals. It will multi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H15/18C08B37/00
CPCC07H15/18C07H1/08C08B37/0003Y02P20/54
Inventor 张宝堂廖娜谭家忠范斌
Owner HUNAN NUSTREETCARAX
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