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A method for extracting Roseville from rhodiola rosea

A crude extraction technology of rose bengal, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of insufficient stability, low product purity, large amount of extractant, etc., and achieve less reagent consumption, High yield and purity in a short time

Active Publication Date: 2015-10-07
三原金瑞生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amount of extractant used in this preparation method is large, and the content of the final product salidroside and rosevin is 5% to 95%, which is not stable enough. Experiments have proved that it is basically no more than 50%, and the product purity is low.

Method used

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  • A method for extracting Roseville from rhodiola rosea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Roseville extract from Rhodiola rosea, the steps are as follows:

[0023] 1) Using Rhodiola rosea as the raw material, reflux extraction twice with 65% ethanol solution 9 times the weight of the raw material, 2 hours each time, combine the extracts, concentrate the extracts under reduced pressure to 2 times the weight of the raw materials, centrifuge, and The clear liquid passes through the chromatographic column filled with D101 macroporous resin, the flow rate of the upper column is 1BV / h, and washed with water (the flow rate of water washing is 0.8BV / h) until the effluent is clear, first eluted with 20% ethanol solution, and then with 65% Elution with ethanol solution, collecting the eluate of 65% ethanol solution, concentrating under reduced pressure, and drying to obtain the crude extract;

[0024] 2) Use dichloromethane as the extractant to perform subcritical extraction on the crude extract. The injection volume of the extractant is 1.5 times the weight of the cr...

Embodiment 2

[0026] Roseville extract from Rhodiola rosea, the steps are as follows:

[0027] 1) Using Rhodiola Rosea as the raw material, reflux extraction with 65% ethanol solution 8 times the weight of the raw material for 3 times, 1 hour each time, combine the extracts, concentrate the extracts under reduced pressure to 2 times the weight of the raw materials, centrifuge, and The supernatant was passed through the chromatographic column filled with D101 macroporous resin, the flow rate of the upper column was 1.2BV / h, washed with water (the flow rate of water washing was 0.5BV / h) until the effluent was clear, first eluted with 20% ethanol solution, and then washed with 65 % ethanol solution for elution, collect the eluate of 65% ethanol solution, concentrate under reduced pressure, and dry to obtain the crude extract;

[0028] 2) Use dichloromethane as the extractant to perform subcritical extraction on the crude extract. The injection volume of the extractant is twice the weight of th...

Embodiment 3

[0030] Roseville extract from Rhodiola rosea, the steps are as follows:

[0031] 1) With Rhodiola rosea as the raw material, reflux extraction once with 70% ethanol solution 9 times the weight of the raw material, for 2 hours each time, concentrate the extract under reduced pressure to 2.5 times the weight of the raw material, centrifuge, and the supernatant through the filling For the chromatographic column with D101 macroporous resin, the flow rate of the upper column is 0.8BV / h, wash with water (the flow rate of water washing is 0.8BV / h) until the effluent is clear, first elute with 25% ethanol solution, and then wash with 70% ethanol solution Remove, collect the eluate of 70% ethanol solution, concentrate under reduced pressure, and obtain the crude extract after drying;

[0032] 2) Use dichloromethane as the extractant to perform subcritical extraction on the crude extract. The injection volume of the extractant is 1 times the weight of the crude extract. The extraction t...

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Abstract

The invention discloses a method for extracting rosavin from Rhodiola rosea. The method includes the following steps that 1) the Rhodiola rosea is used as a raw material, a 60%-70% ethanol solution is used for conducting reflux extraction, after an extracting solution is concentrated, centrifuging is carried out, a liquid supernatant is washed until effluent is clarified through a chromatographic column filled with macroporous resin, elution is carried out first by using a 15%-25% ethanol solution, then elution is carried out again by using a 60%-70% ethanol solution, and after concentration and drying are completed, a crude extract is obtained; 2) subcritical extraction is conducted on the crude extract by using dichloromethane as an extraction agent, the injection rate of the extraction agent is one to two times of the weight of the crude extract, the extraction temperature is 38-42 DEG C, the extraction pressure is 2.3-2.7MPa, the extraction time is 20-40min, and after the self-volatilized dichloromethane is recycled, a finished product can be obtained. The method for extracting the rosavin from the Rhodiola rosea is simple in step, short in used time and few in dosage of reagents, the yield is about 1.5%, the content of the rosavin is about 80%, the yield and the purity are high, and therefore the method is more suitable for industrial production.

Description

technical field [0001] The invention relates to a method for preparing a plant extract, in particular to a method for extracting roseavi from rhodiola rosea. Background technique [0002] Rosavin is a phenylpropanoid glycoside with molecular formula C 20 h 28 o 10 , the molecular structure is: [0003] [0004] Roseville is mainly used for anti-fatigue, anti-hypoxia, stress relief, improvement of work efficiency and treatment of functional diseases of the nervous system. The main source of Roseville is Rhodiola rosea (RhodioLa rosea L.), especially its rhizome. Rhodiola rosea, also known as Rhodiola rosea, is a plant of the genus Rhodiola in the sedum family. It is a rare medicinal plant resource with excellent health benefits. It contains a variety of physiologically active ingredients, such as rosevi, tyrosol, flavonoids etc. The content of Roseville in Rhodiola rosea is less, how to obtain high yield and high purity Roseville from Rhodiola rosea is a research hot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/18C07H1/08
Inventor 文继承蒋元勇朱昂辉易华李丽金雍小军
Owner 三原金瑞生物工程有限公司
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