The invention discloses a health care wine for fatigue endurance and sleep regulation and a method for preparing the health care wine. The health care wine for fatigue endurance and sleep regulation is formed by brewing the following raw materials in the following proportions: 4000-8000 g of fresh grapes, 4000-8000 g of fresh mulberries, 50-100 g of astragali radix, 50-100 g of virgin soil, 20-30g of white prepared lateral root of aconite, 20-30 g of cooked rhubarb, 20-30 g of honey-fried licorice root, 300-500 g of ginger, 100-200 g of sweet osmanthus, 9-15 g of notopterygium root, 1000-3000 g of Chinese date, 2000-3000 g of lily, 2000-4000 g of fushen, 20-30 g of amomum cardamomum and 60-100 L of white spirit. The health care wine is capable of enduring fatigue, regulating sleep, recuperating liver and kidney, settling the viscera and enhancing the disease resistance of a human body, and has a good regulation effect for laziness, fatigue, insomnia and dreaminess, soreness and weakness of waist and knees, muscle weakness, hyposexuality, hair loss and the like caused by life stress.