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Method for synthesizing salidroside by utilizing enzyme catalyzed direct glucosylation

A technology of salidroside and chemical synthesis, applied in chemical recovery, fermentation, etc., to achieve the effects of reducing water activity, improving solubility, and high purity

Inactive Publication Date: 2011-09-07
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for synthesizing salidroside by enzyme-catalyzed direct glycosylation in view of the defects of the existing reaction system. The method is simple to operate and improves the efficiency of enzyme-catalyzed synthesis of salidroside

Method used

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  • Method for synthesizing salidroside by utilizing enzyme catalyzed direct glucosylation

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Add 55 μL of citric acid-phosphate buffer solution, 396 μL 1,4-dioxane and 99 μL 1-butyl-3-methylimidazolium hexafluorophosphate in a 10 ml test tube, add 22 mg glucose, 33 mg tyrosol to the reaction medium , adding 1.1 mg of enzyme to initiate the reaction, shaking the reaction on a rotary shaker at 200 rpm for 2 days.

[0034] Sampling 550μL at the scheduled time, adding 8.5ml of methanol to terminate the reaction and ultrasonically oscillating for 20min, centrifuging at 14000rpm for 5min, filtering the supernatant with a 0.45um filter membrane, and using high performance liquid chromatography (LC-10A) to determine the yield of salidroside To measure. SPD-10A UV-visible detector; Elite C18 (4.6mm×250mm×5um); mobile phase methanol:water (15:85), flow rate 1ml / min; detection wavelength 275nm; column temperature room temperature; injection volume 20μL. The salidroside concentration in the reaction system was detected to be 3.6g / L.

Embodiment 2

[0036] Add 55 μL of citric acid-phosphate buffer solution, 500 μL of 1-hexyl-3-methylimidazolium hexafluorophosphate in a 10 ml test tube, add 22 mg of glucose, 33 mg of tyrosol, and 1.1 mg of enzyme to initiate the reaction at 200 rpm Shake the reaction on a rotary shaker for 2 days. The product salidroside was detected by the same detection method as in Example 1, and the product concentration could reach 0.58 g / L.

Embodiment 3

[0038] Add 55 μL of citric acid-phosphate buffer solution, 396 μL 1,4-dioxane and 99 μL 1-butyl-3-methylimidazolium hexafluorophosphate in a 10 ml test tube, add 22 mg glucose, 99 mg tyrosol to the reaction medium , adding 1.1 mg of enzyme to initiate the reaction, shaking the reaction on a rotary shaker at 200 rpm for 2 days. The product salidroside was detected by the same detection method as in Example 1, and the product concentration could reach 7.9 g / L.

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Abstract

The invention relates to a method for synthesizing salidroside by utilizing enzyme catalyzed direct glucosylation, comprising the following steps: carrying out a reaction on glucose, tyrosol and enzyme in a solvent containing a buffer solution; and collecting the salidroside by adopting the conventional method, wherein, the solvent comprises an icon-containing liquid and an organic solvent. Compared with the existing method, in the method, a reaction medium composed of the ionic liquid is adopted as a reaction system for synthesizing the salidroside by utilizing the enzyme catalyzed direct glucosylation; the enzyme activity and the enzyme stability are ensured; the dissolubility of the substrate in the reaction system is improved; the water activity in the reaction system is reduced; the expensive glucoside donor needs not to be used, and the salidroside can be further synthesized; the separation and purification processes of the product are simple, the unreacted tyrosol can be recovered and recycled, thus lowering the production cost, and satisfying the requirements of rapidly developing the medical industries.

Description

technical field [0001] The invention belongs to the field of biocatalysis, and more specifically relates to a method for synthesizing salidroside through direct glycosylation by enzyme catalysis. Background technique [0002] Salidroside is an alcohol glycoside formed by the dehydration of the hemiacetal hydroxyl group of sugar and alcohol hydroxyl group. It is a glycoside formed by combining glucose and tyrosol with glycosidic bonds. Tyrosol is its aglycon. It is the traditional Chinese medicine Rhodiola rosea The main active ingredients in it have many functions such as anti-hypoxia, anti-cold, anti-fatigue, anti-radiation and anti-virus, and also have the effects of delaying the aging of the body and preventing senile diseases, especially in military medicine, aerospace medicine, sports medicine, etc. It has very important application value in health care and medicine. [0003] The traditional way to obtain salidroside is mainly to extract the plant rhodiola as raw mater...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44
CPCY02P20/582
Inventor 石贤爱岳磊林琼妹许格林磊郭养浩
Owner FUZHOU UNIV
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