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Process for preparing high purity rhodiola root glycosides

A salidroside, high-purity technology, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of environmental pollution, high price, high toxicity, etc., and achieve high yield and high purity. , large-scale effect

Inactive Publication Date: 2007-07-04
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above studies, no matter whether the normal-phase silica gel column preparative chromatography or the high-speed countercurrent chromatography technology is used, there are the following defects: a large amount of low-polarity organic solvents are used, and the toxicity is relatively high, especially the normal-phase silica gel column preparative chromatography, the silica gel filler used , are often discarded without recycling after use, causing environmental pollution. Moreover, the use of high performance liquid chromatography requires expensive instruments, and the amount of preparation is limited, and generally can only achieve milligram-level separation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take Rhodiola plants as raw materials, crush them to below 10 mesh, add 2 times of water to decoct twice, each time for 1 hour, combine the aqueous solutions, concentrate to 1 / 10 volume under reduced pressure, and obtain red Aqueous extract of sedroside.

[0025] Take the above 200 grams of Rhodiola rosea water extract, dissolve it with a small amount of water, add 95% ethanol until the polysaccharide precipitates completely, filter, concentrate the solution until there is no alcohol smell, apply a macroporous adsorption resin, and elute with water, about 4 to 5 times the column volume, and then eluted with 5% ethanol aqueous solution, collected fractions of this concentration, concentrated under reduced pressure until there was no alcohol smell, put on a reversed-phase C18 silica gel column, first eluted with water, then eluted with 10% alcohol, and used For HPLC detection, the parts containing salidroside were collected, concentrated under reduced pressure to remove m...

Embodiment 2

[0027] Take Rhodiola plants as raw materials, crush them to below 10 mesh, add 10 times of water to decoct 3 times, each time is 2 hours, combine the aqueous solutions, concentrate to 1 / 20 volume under reduced pressure, and obtain red Aqueous extract of sedroside.

[0028] Take the above 200 grams of Rhodiola rosea water extract, dissolve it with a small amount of water, add 95% ethanol until the polysaccharide precipitates completely, filter, concentrate the solution until it has no alcohol smell, then extract it with chloroform for 3 times, remove the chloroform from the water layer under reduced pressure, and put Macroporous adsorption resin, eluted with water, about 4 to 5 times the column volume, and then changed to 10% methanol aqueous solution for elution, collected fractions of this concentration, concentrated under reduced pressure to remove methanol, put on a reversed-phase C8 silica gel column, and first used water Elution, then use 5% ethanol to elute, use HPLC to ...

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Abstract

The invention relates to a method for separating high-purity solidroside from red common stonecrop herb, belonging to Chinese traditional medicine preparation field. The method comprises following steps: extracting raw material with hot water or water containing no more than 50% of methanol or ethanol; condensing the extrant and presipitaing it with ethanol; eluting with water solution containing no more than 10% of methanol or ethanol through chromatographic column of polymeric packing stationary phase; separating the product with chromatographic column of bonded stationary phase silica gel packing material and getting high-purity solidroside. The invention is characterized by large produtino scale, high production rate, high purity, repeated use of polymeric packing stationary phase and bonded silica gel packing stationary phase, and no volatile, toxic nd polluting containing organic solvent.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to a method for separating high-purity salidroside from rhodiola rosea. Background technique [0002] Rhodila is a perennial herb with anti-aging, anti-hypoxia, anti-fatigue, immune regulation and other effects, and is of great significance in military medicine, aerospace medicine, sports medicine and health care medicine. The main components in Rhodiola are glycosides, flavonoids, coumarins, amino acids, tannins and volatile oils. The main component salidroside (Salidroside) is often used as a quality control indicator, which has anti-oxidation, anti-radiation, anti-hypoxia, anti-fatigue and other effects. At present, the main methods for preparing salidroside include chemical synthesis and extraction from plants of the genus Rhodiola. The key steps of the method for separating high-purity salidroside from Rhodiola rosea that have been reported at present all ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18C07H1/08
Inventor 俞培忠丁溪影刘珊林
Owner FUDAN UNIV
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