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Cultivation method and plant application of plant including salidroside

The technology of a sedroside plant and its cultivation method, which is applied in the field of biotransformation, can solve the problems of low yield of salidroside, complex chemical synthesis methods, and shortage of resources, and achieve the effects of protecting the liver, enhancing immunity, and protecting the cardiovascular system

Inactive Publication Date: 2014-08-27
HUNAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a cultivation method and plant application of salidroside-containing plants, so as to solve the problem of resource shortage caused by the low yield of salidroside and complicated chemical synthesis methods.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: Take Compositae lettuce, cultivate it according to conventional cultivation methods, transplant it when 5-6 true leaves grow, and add 10g tyrosol per kilogram of soil into the transplanted soil (you can also add tyrosol Alcohol is dissolved in water and added to the soil, and mixed), and every 3 to 15 days (the initial interval is 15 days, and then gradually shorten the interval to 3 days) to apply water containing tyrosol to the soil, and can also be mixed with organic fertilizers Adding amount is 10g tyrosol per kilogram of soil, stop adding tyrosol 3 days before harvesting, so that allow tyrosol to transform completely, lettuce after harvesting, measure salidroside content according to the above method, its content (fresh weight ) up to 0.15% or more.

[0037]Detection method of salidroside: adopt high-performance liquid chromatography, take 0.5g of the sample to be tested, add 25ml of 50% methanol, weigh it, heat and reflux for 30 minutes, let it cool,...

Embodiment 2

[0038] Embodiment 2, get Compositae lettuce, cultivate according to conventional cultivation methods, transplant when 5 to 6 true leaves grow, add 0.1g tyrosol per kilogram in the transplanted soil (tyrosol can be added Add to the soil after dissolving in water and mix well), apply tyrosol-containing water to the soil where it grows every 5 days after transplanting, and the amount of tyrosol added is twice that of the previous time (that is, each kilogram of soil 0.2, 0.4, 0.8, 1.6, 3.2, 6.4, 12.8g, after reaching 12.8g, the concentration will no longer increase, and it will be 12.8g every time thereafter). Stop adding tyrosol 5 days before harvesting, so as to allow complete conversion of tyrosol. The content of salidroside in lettuce after harvesting can be determined according to the above method, and its content (fresh weight) can reach up to 0.18% or more.

Embodiment 3

[0039] Embodiment 3, get Compositae lettuce, cultivate according to conventional cultivation methods, transplant when 5-6 true leaves grow, add 2g tyrosol per kilogram in the soil for transplanting (tyrosol can be mixed with water After dissolving, add to the soil and mix well), apply tyrosol-containing water to the soil every 7 days, or mix it with organic fertilizer for application, the addition amount is 1g tyrosol per kg of soil, stop adding 1 day before harvest Tyrosol, in order to allow the complete conversion of tyrosol, the content of salidroside in the harvested lettuce is determined according to the above method, and its content (fresh weight) can reach up to 0.15% or more.

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PUM

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Abstract

The invention provides a cultivation method and plant application of a plant including salidroside. Tyrosol is added to plant growth solid mediums such as soil, ground substance and fertilizer, 0.1-100g tyrosol is added for one kilogram of the solid mediums or 0.1-1000g tyrosol is added for every plant, and the tyrosol is supplemented at certain time interval or not. The tyrosol taken by the plant is converted into salidroside under the action of related enzymes in cells, so that the plant can obtain physiological and pharmacological activity of the salidroside. The plant can be used directly and can be made into health food, drug, food additives and the like or can be used as a raw material for extracting the salidroside.

Description

technical field [0001] The invention relates to the technical field of biotransformation, in particular to a cultivation method and plant application of salidroside-containing plants. Background technique [0002] Salidroside (salidroside) is the most important medicinal ingredient of Crassulaceae (Crassulaceae) Rhodiola (Rhodiola L.) plants. Protect nerve cells, protect the liver, anti-oxidation, anti-tumor, anti-radiation and other specific effects. It has extremely important application value in military, aerospace, sports and health care medicine. In recent years, there are more and more types of products such as pharmaceuticals, beverages, food and cosmetics produced with it as the main raw material, and people's demand for salidroside is increasing. [0003] Rhodiola plants are the main source of extracting salidroside, but wild resources are scarce, and the content of salidroside is very low, such as the most commonly used Rhodiola alpine and Rhodiola grandiflora, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/06A23L1/29A23L1/30C07H15/18C07H1/08A61K36/39A61K36/31A61K36/81A61K36/28A61P9/10A61P25/28A61P9/00A61P39/00A61P37/04A61P25/00A61P1/16A61P39/06A61P35/00A61P17/16A23L33/00
Inventor 谭朝阳彭菲崔培梧于静罗跃龙徐德宏孟蕾
Owner HUNAN UNIV OF CHINESE MEDICINE
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