Glossy privet fruit total triterpenes extract and total phenols extract as well as preparation method thereof

A technology of extract, Ligustrum lucidum, applied in the field of medicine

Inactive Publication Date: 2009-01-21
石任兵
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Glossy privet fruit total triterpenes extract and total phenols extract as well as preparation method thereof
  • Glossy privet fruit total triterpenes extract and total phenols extract as well as preparation method thereof
  • Glossy privet fruit total triterpenes extract and total phenols extract as well as preparation method thereof

Examples

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Embodiment 1

[0070] Example 1: Preparation process of Ligustrum lucidum triterpene extract and total phenolic extract

[0071] Get 1 kg of Ligustrum lucidum decoction pieces, reflux extraction with 75% ethanol (10 times the amount) for 3 times, extract for 1 hour each time, reclaim the solvent under reduced pressure to obtain the extract, add a certain volume of water to disperse and dissolve, so that the amount of medicinal material is the same as that of the solution after dispersion. The volume ratio is 1:10. Then centrifuge at a speed of 4000 rpm for 30 minutes, pour out the supernatant, add 95% ethanol 7 times the amount of the crude drug to disperse the precipitate, and centrifuge for 30 minutes at a speed of 3000 rpm. After centrifugation, recover the solvent from the supernatant to dryness, and vacuum-dry to obtain the triterpene extract of Ligustrum lucidum. The supernatant was passed through 4L AB-8 macroporous adsorption resin, the adsorption flow rate was 1.0ml / min, the diamet...

Embodiment 2

[0073] Embodiment 2: Ligustrum lucidum triterpene extract and total phenolic extract preparation process

[0074] Take 1kg of Ligustrum lucidum decoction pieces, extract 3 times with 10L of 75% ethanol under reflux, extract for 1 hour each time, recover the solvent under reduced pressure to obtain the extract, add a certain volume of water to disperse and dissolve, so that the volume ratio of the amount of medicinal material to the solution after dispersion is 1 : 10. Then centrifuge at a speed of 3000 rpm for 30 minutes, pour out the supernatant, disperse the precipitate with 95% ethanol 5 times the amount of the crude drug, and centrifuge for 30 minutes at a speed of 3000 rpm. After centrifugation, recover the solvent from the supernatant to dryness, and vacuum-dry to obtain the triterpene extract of Ligustrum lucidum. The supernatant was passed through 4L AB-8 macroporous adsorption resin, the adsorption flow rate was 1.0ml / min, the diameter-to-height ratio of the resin co...

Embodiment 3

[0076] Embodiment 3: Ligustrum lucidum triterpene extract and total phenolic extract preparation process

[0077] Take 1kg of Ligustrum lucidum decoction pieces, extract 3 times with 12L of 75% ethanol under reflux, extract for 1 hour each time, recover the solvent under reduced pressure to obtain the extract, add a certain volume of water to disperse and dissolve, so that the volume ratio of the amount of medicinal material to the solution after dispersion is 1 : 10. Then centrifuge at a speed of 4000 rpm for 30 minutes, pour out the supernatant, add 95% ethanol 7 times the amount of crude drug to the precipitate to disperse, and centrifuge at a speed of 4000 rpm for 20 minutes. After centrifugation, recover the solvent from the supernatant to dryness, and vacuum-dry to obtain the triterpene extract of Ligustrum lucidum. The supernatant was passed through 4L AB-8 macroporous adsorption resin, the adsorption flow rate was 1.0ml / min, the diameter-to-height ratio of the resin c...

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Abstract

The invention discloses total triterpene extract and total phenol extract extracted from Chinese herbal medicine glossy privet fruit and a preparation method thereof. The total triterpene extract mainly contains oleanolic acid, ursolic acid, acetyl oleanolic acid, indicant compound and other derivatives with acetyl oleanolic acid as the mother nucleus. The total phenol extract mainly contains hydroxyl mandelic, 3, 4-dihydroxy phenylethyl alcohol, salidroside and indicant compound and other derivatives with salidroside as the mother nucleus. The glossy privet fruit total triterpene extract and total phenol extract can be prepared by any method of solvent distillation, solvent extraction, deposition, macroporous resin absorption, supercritical fluid extraction, column chromatography and liquid-liquid countercurrent distribution chromatography, or any combination of these methods. The total percentage of various triterpenoids of the prepared glossy privet fruit total triterpene extract is 5 to 100 percent (w/w); wherein, the content of oleanolic acid and ursolic acid accounts for 5 to 100 percent (w/w) of the total triterpene. The total percentage of various phenols of the prepared glossy privet fruit total phenol extract is 5 to 100 percent (w/w); wherein, the content of salidroside accounts for 5 to 100 percent (w/w) of the total phenol.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a total triterpene extract and a total phenol extract of Ligustrum lucidum, a preparation method, a quality control method and its application in the fields of medicine and health food. Background technique [0002] Ligustrum lucidum Ait., a commonly used traditional Chinese medicine, is derived from the dried mature fruit of Ligustrum lucidum Ait., a plant of Oleaceae (Oleaceae). Traditional Chinese medicine theory believes that Ligustrum lucidum is good at nourishing liver and kidney yin, and its name is black hair. It not only treats dizziness, weak waist and knees, dark eyesight, premature graying of beard and hair caused by liver and kidney yin deficiency, but also cures symptoms such as fever due to yin deficiency. Modern pharmacological studies have shown that Ligustrum lucidum has anti-inflammatory, anti-tumor, anti-cancer, anti-bacterial, anti-aging, hypogly...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P1/10A61P7/00A61P9/06A61P9/10A61P11/14C07C37/68C07H1/08C07J9/00C07J63/00A61K131/00A61K125/00A61K127/00A61K133/00A61K135/00
CPCY02P20/54
Inventor 石任兵田恒康周文娟刘斌姜艳艳
Owner 石任兵
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