Ergothioneine anti-aging skin care composition and preparation method thereof

Abstract

The invention belongs to the field of cosmetics, and particularly discloses an ergothioneine anti-aging skin care composition and a preparation method thereof. According to the formula, each 100 g of the anti-allergy anti-inflammatory and anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti The transdermal rate and the stability of the ergothioneine and the pyrroloquinoline quinone are improved by adopting a liposome encapsulation technology, an'enzymatic + non-enzymatic 'dual-mode antioxidant system is formed by combining the French muskmelon extract and the roxburgh rose extract, full-type free radical scavenging is covered, and cell autophagy is regulated and controlled through spermidine to delay senescence.

Description

Technical Field

[0001] The present invention belongs to the field of cosmetics, and particularly relates to an ergothioneine anti-aging skin care composition and a preparation method thereof. Background Art

[0002] With the development of society and the improvement of people's living standards, skin care has gradually become an indispensable part of modern life. In particular, anti-aging skin care has attracted more and more attention from consumers. The manifestations of aging are not only reflected in appearance such as wrinkles, skin laxity and pigmentation, but also affect an individual's self-confidence and mental health. Therefore, how to effectively anti-aging has become an important research direction in the research and development of skin care products;

[0003] In the field of anti-aging skin care, traditional technologies mainly rely on single antioxidant components, such as vitamin C and vitamin E, which have limitations:

[0004] Poor component stability: For example, vitamin C is easily oxidized and inactivated, and high concentrations need to be added, resulting in increased irritation;

[0005] Low transdermal efficiency: Most antioxidants are difficult to penetrate the stratum corneum due to their high molecular polarity;

[0006] Single mechanism of action: Single components only target specific free radicals, such as hydroxyl radicals, and cannot cover multiple types of oxidative stress sources such as superoxide anions and hydrogen peroxide. Summary of the Invention

[0007] The purpose of the present invention is to provide an ergothioneine anti-aging skin care composition and a preparation method thereof, so as to solve the problem that anti-aging compositions mostly rely on single-functional components and are difficult to penetrate the stratum corneum as mentioned in the above background art.

[0008] To achieve the above purpose, the present invention provides the following technical solutions:

[0009] An ergothioneine anti-aging skin care composition contains the following components per 100 g of the formula: 10-20 mg of ergothioneine, 5-15 mg of French melon extract, 40-60 mg of Rosa roxburghii extract, 3-8 mg of spermidine, 8-12 mg of pyrroloquinoline quinone nanoliposomes, 0.1-0.5 g of niacinamide, 0.05-0.2 g of sodium hyaluronate, 0.05-0.1 g of ectoine, 0.05-0.1 g of xanthan gum, 0.1-0.2 g of carbomer, and the balance is a cosmetically acceptable matrix, including an aqueous phase and an oil phase.

[0010] Preferably, per 100 g of the formulation, it contains the following components: ergothioneine 15 mg, French melon extract 10 mg, Rosa roxburghii Tratt extract 50 mg, spermidine 5 mg, pyrroloquinoline quinone nanoliposome 10 mg, niacinamide 0.3 g, sodium hyaluronate 0.1 g, ectoine 0.08 g, xanthan gum 0.08 g, carbomer 0.1 g, and the balance is a cosmetically acceptable matrix, including an aqueous phase and an oil phase.

[0011] A preparation method of an ergothioneine anti-aging skin care composition includes the following steps:

[0012] S1. Dissolve ergothioneine, French melon extract, and Rosa roxburghii Tratt extract in an aqueous phase matrix at a temperature of 35 ± 2 °C, stir at 300 rpm for 10 minutes, then add spermidine, and treat it under ultrasonic conditions of 40 kHz until completely dissolved to obtain a spermidine-aqueous phase mixture;

[0013] S2. Slowly inject pyrroloquinoline quinone nanoliposome into the spermidine-aqueous phase mixture, and stir at 200 - 400 rpm for 20 minutes under light-shielded conditions to form an active ingredient complex solution;

[0014] S3. Preheat the oil phase matrix to 40 °C, then add an emulsifier to the active ingredient complex solution, and perform high-speed shear emulsification at 10000 - 12000 rpm for 5 minutes in a vacuum emulsification tank with a vacuum degree of -0.08 MPa to form a primary emulsion. Transfer the primary emulsion to a high-pressure homogenizer and circulate it 3 times under a pressure of 20 MPa to obtain a stable emulsion with a particle size ≤ 5 μm;

[0015] S4. Cool the stable emulsion to below 30 °C, sequentially add niacinamide, sodium hyaluronate, ectoine, and azone, and adjust the pH to 5.8 - 6.2 with sodium citrate buffer solution to form a final product matrix;

[0016] S5. Add xanthan gum and carbomer to the final product matrix, stir at 500 rpm at 25 °C until the viscosity reaches 3000 - 5000 mPa·s, and then filter through a 0.22 μm microporous membrane to obtain the composition.

[0017] Preferably, the preparation process of the Rosa roxburghii Tratt extract is as follows:

[0018] Mix Rosa roxburghii Tratt fruit powder with 70% ethanol at a solid-liquid ratio of 1:10, extract at 40 - 50 °C and an ultrasonic power of 200 W for 30 minutes, filter, and then concentrate under reduced pressure until the solid content ≥ 30% to obtain the Rosa roxburghii Tratt extract.

[0019] Preferably, the preparation parameters of the pyrroloquinoline quinone nanoliposome are:

[0020] Dissolve hydrogenated lecithin and cholesterol in a phosphate buffer solution with a pH of 7.4 at a mass ratio of 4:1. After adding pyrroloquinoline quinone, transfer it to a high-pressure homogenizer and circulate it 3-5 times at a pressure of 800-1000 bar. The particle size of the nanoliposomes is 80-120 nm, and the encapsulation efficiency is ≥85%.

[0021] Preferably, the preparation process of the French melon extract is as follows:

[0022] After the melon pulp is crushed, under the conditions of pH 6.5-7.0 and temperature 4-8 °C, enzymolysis is carried out with cellulase with an enzyme activity of ≥5000 U / g for 2-4 hours;

[0023] The enzymolysis solution is separated by a 50 kDa ultrafiltration membrane, and the active fragment with a molecular weight <50 kDa is collected. After freeze-drying, the French melon extract is obtained.

[0024] Preferably, the emulsifier in step S3 is polysorbate-80, and the mass ratio is 0.5-1 g.

[0025] Preferably, the temperature in the primary emulsification stage in step S3 is 40 ± 1 °C, and the temperature is reduced to 35 ± 1 °C in the homogenization stage.

[0026] Preferably, the aqueous phase matrix includes one of deionized water, glycerol, and 1,3-propanediol, and the oil phase matrix includes one of caprylic acid, glyceryl tributyrate, squalane, and tocopheryl acetate.

[0027] Compared with the prior art, the beneficial effects of the present invention are:

[0028] The present invention improves the transdermal rate and stability of ergothioneine and pyrroloquinoline quinone by adopting the liposome encapsulation technology, combines the French melon extract and the Rosa roxburghii extract to form an "enzymatic + non-enzymatic" dual-mode antioxidant system to cover the scavenging of all types of free radicals, delays aging by regulating autophagy with spermidine, activates mitochondrial biosynthesis with PQQ to enhance cell energy metabolism, introduces Ectoin and low-molecular-weight sodium hyaluronate, and constructs a "water-locking - protection" double-layer barrier to reduce transdermal water loss. Detailed Embodiments

[0029] To make the above objects, features, and advantages of the present invention more obvious and understandable, the following detailed description of the specific embodiments of the present invention is provided.

[0030] In the following description, many specific details are set forth to fully understand the present invention. However, the present invention can also be implemented in other ways different from those described herein. Those skilled in the art can make similar promotions without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed below.

[0031] Second, the "one embodiment" or "embodiment" referred to herein means a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The appearances of "in one embodiment" in different places in this specification do not all refer to the same embodiment, nor are they separate or alternative embodiments that are mutually exclusive of other embodiments.

[0032] Embodiment 1: This embodiment provides an ergothioneine anti-aging skin care composition, which contains the following components per 100 g of the formula: 15 mg of ergothioneine, 10 mg of French melon extract, 50 mg of rose hip extract, 5 mg of spermidine, 10 mg of pyrroloquinoline quinone nanoliposome, 0.3 g of nicotinamide, 0.1 g of sodium hyaluronate, 0.08 g of ectoine, 0.08 g of xanthan gum, 0.1 g of carbomer, and the balance is a cosmetically acceptable matrix, including an aqueous phase and an oil phase.

[0033] A preparation method of an ergothioneine anti-aging skin care composition includes the following steps:

[0034] S1. Dissolve ergothioneine, French melon extract, and rose hip extract in an aqueous phase matrix at a temperature of 35 ± 2 °C, stir at 300 rpm for 10 minutes, then add spermidine, and treat it under ultrasonic conditions of 40 kHz until completely dissolved to obtain a spermidine-aqueous phase mixture;

[0035] S2. Slowly inject pyrroloquinoline quinone nanoliposome into the spermidine-aqueous phase mixture, and stir at 200 - 400 rpm for 20 minutes under light-shielded conditions to form an active ingredient complex solution;

[0036] S3. Preheat the oil phase matrix to 40 °C, then add an emulsifier to the active ingredient complex solution, and perform high-speed shear emulsification at 10000 - 12000 rpm for 5 minutes in a vacuum emulsifying tank with a vacuum degree of -0.08 MPa to form a primary emulsion. Transfer the primary emulsion to a high-pressure homogenizer and circulate it 3 times under a pressure of 20 MPa to obtain a stable emulsion with a particle size ≤ 5 μm;

[0037] S4. Cool the stable emulsion to below 30 °C, sequentially add nicotinamide, sodium hyaluronate, ectoine, and azone, and adjust the pH to 5.8 - 6.2 with sodium citrate buffer solution to form a final product matrix;

[0038] S5. Add xanthan gum and carbomer to the final product matrix, stir at 500 rpm at 25 °C until the viscosity reaches 3000 - 5000 mPa·s, and then filter through a 0.22 μm microporous membrane to obtain the composition.

[0039] Specifically, the preparation process of the rose hip extract is as follows:

[0040] The Rosa roxburghii Tratt fruit powder was mixed with 70% ethanol at a solid-liquid ratio of 1:10, extracted for 30 minutes at 40 - 50 °C under an ultrasonic power of 200 W, filtered, and then concentrated under reduced pressure to a solid content of ≥30% to obtain the Rosa roxburghii Tratt extract.

[0041] Specifically, the preparation parameters of the pyrroloquinoline quinone nano-liposomes are as follows:

[0042] Hydrogenated lecithin and cholesterol were dissolved in a pH 7.4 phosphate buffer solution at a mass ratio of 4:1. After adding pyrroloquinoline quinone, it was transferred to a high-pressure homogenizer and circulated 3 - 5 times under a pressure of 800 - 1000 bar. The particle size of the nano-liposomes was 80 - 120 nm, and the encapsulation efficiency was ≥85%.

[0043] Specifically, the preparation process of the French melon extract is as follows:

[0044] After the melon pulp was crushed, it was enzymatically hydrolyzed with cellulase with an enzyme activity of ≥5000 U / g for 2 - 4 hours at pH 6.5 - 7.0 and a temperature of 4 - 8 °C;

[0045] The enzymatic hydrolysate was separated by a 50 kDa ultrafiltration membrane, and the active fragment with a molecular weight <50 kDa was collected and freeze-dried to obtain the French melon extract.

[0046] Specifically, the emulsifier in step S3 is polysorbate - 80, and the mass ratio is 0.5 g.

[0047] Specifically, the temperature in the primary emulsification stage in step S3 is 40 ± 1 °C, and the temperature is reduced to 35 ± 1 °C in the homogenization stage.

[0048] Specifically, the aqueous phase matrix includes one of deionized water, glycerol, and 1,3 - propanediol, and the oil phase matrix includes one of caprylic acid, glyceryl tributyrate, squalane, and tocopheryl acetate.

[0049] Example 2: This example provides an ergothioneine anti-aging skin care composition, which contains the following components per 100 g of the formula: 10 mg of ergothioneine, 5 mg of French melon extract, 50 mg of Rosa roxburghii Tratt extract, 5 mg of spermidine, 8 mg of pyrroloquinoline quinone nano-liposomes, 0.3 g of nicotinamide, 0.1 g of sodium hyaluronate, 0.08 g of ectoine, 0.08 g of xanthan gum, 0.1 g of carbomer, and the balance is a cosmetically acceptable matrix, including an aqueous phase and an oil phase.

[0050] A method for preparing an ergothioneine anti-aging skin care composition, comprising the following steps:

[0051] S1. Dissolve ergothioneine, French melon extract, and Rosa roxburghii Tratt extract in an aqueous matrix at a temperature of 35 ± 2 °C. After stirring at 300 rpm for 10 minutes, add spermidine and treat it under ultrasonic conditions of 40 kHz until completely dissolved to obtain a spermidine-aqueous phase mixture;

[0052] S2. Slowly inject pyrroloquinoline quinone nanoliposomes into the spermidine-aqueous phase mixture and stir at 200 - 400 rpm for 20 minutes under light-shielded conditions to form an active ingredient complex solution;

[0053] S3. Preheat the oil phase matrix to 40 °C, then add it and an emulsifier to the active ingredient complex solution. High-speed shear emulsify at 10000 - 12000 rpm for 5 minutes in a vacuum emulsifying tank with a vacuum degree of -0.08 MPa to form a primary emulsion. Transfer the primary emulsion to a high-pressure homogenizer and circulate it 3 times under a pressure of 20 MPa to obtain a stable emulsion with a particle size ≤ 5 μm;

[0054] S4. Cool the stable emulsion to below 30 °C, sequentially add niacinamide, sodium hyaluronate, ectoine, and azone, and adjust the pH to 5.8 - 6.2 with sodium citrate buffer solution to form a final product matrix;

[0055] S5. Add xanthan gum and carbomer to the final product matrix, stir at 500 rpm at 25 °C until the viscosity reaches 3000 - 5000 mPa·s, and then filter through a 0.22 μm microporous filter membrane to obtain the composition.

[0056] Specifically, the preparation process of the Rosa roxburghii Tratt extract is as follows:

[0057] Mix Rosa roxburghii Tratt fruit powder with 70% ethanol at a material-liquid ratio of 1:10, extract at 40 - 50 °C and an ultrasonic power of 200 W for 30 minutes, filter, and then concentrate under reduced pressure until the solid content ≥ 30% to obtain the Rosa roxburghii Tratt extract.

[0058] Specifically, the preparation parameters of the pyrroloquinoline quinone nanoliposomes are as follows:

[0059] Dissolve hydrogenated lecithin and cholesterol in a pH 7.4 phosphate buffer solution at a mass ratio of 4:1, add pyrroloquinoline quinone, and then transfer it to a high-pressure homogenizer and circulate it 3 - 5 times under a pressure of 800 - 1000 bar. The particle size of the nanoliposomes is 80 - 120 nm, and the encapsulation efficiency ≥ 85%.

[0060] Specifically, the preparation process of the French melon extract is as follows:

[0061] After crushing the melon pulp, enzymatically hydrolyze it with cellulase with an enzyme activity ≥ 5000 U / g for 2 - 4 hours under the conditions of pH 6.5 - 7.0 and a temperature of 4 - 8 °C;

[0062] The enzymatic hydrolysate was separated by a 50 kDa ultrafiltration membrane, and the active fraction with a molecular weight <50 kDa was collected. After freeze-drying, the French melon extract was obtained.

[0063] Specifically, the emulsifier described in step S3 is polysorbate-80, and the mass ratio is 0.5 g.

[0064] Specifically, the temperature in the primary emulsification stage described in step S3 is 40 ± 1 °C, and the temperature is reduced to 35 ± 1 °C in the homogenization stage.

[0065] Specifically, the aqueous phase matrix includes glycerol, and the oil phase matrix includes tricaprylin.

[0066] Example 3: This example provides an ergothioneine anti-aging skin care composition, which contains the following components per 100 g of the formula: 15 mg of ergothioneine, 10 mg of French melon extract, 30 mg of Rosa roxburghii extract, 5 mg of spermidine, 10 mg of pyrroloquinoline quinone nanoliposome, 0.3 g of niacinamide, 0.1 g of sodium hyaluronate, 0.08 g of ectoine, 0.08 g of xanthan gum, 0.1 g of carbomer, and the balance is a cosmetically acceptable matrix, including an aqueous phase and an oil phase.

[0067] A preparation method of an ergothioneine anti-aging skin care composition includes the following steps:

[0068] S1. Dissolve ergothioneine, French melon extract, and Rosa roxburghii extract in an aqueous phase matrix at a temperature of 35 ± 2 °C, stir at 300 rpm for 10 minutes, then add spermidine, and treat under ultrasonic conditions of 40 kHz until completely dissolved to obtain a spermidine-aqueous phase mixture;

[0069] S2. Slowly inject the pyrroloquinoline quinone nanoliposome into the spermidine-aqueous phase mixture, and stir at 200 - 400 rpm for 20 minutes in the dark to form an active ingredient complex solution;

[0070] S3. Preheat the oil phase matrix to 40 °C, then add it and the emulsifier to the active ingredient complex solution. High-speed shear emulsification is carried out at 10000 - 12000 rpm for 5 minutes in a vacuum emulsification tank with a vacuum degree of -0.08 MPa to form a primary emulsion. Transfer the primary emulsion to a high-pressure homogenizer and circulate it 3 times under a pressure of 20 MPa to obtain a stable emulsion with a particle size ≤5 μm;

[0071] S4. Cool the stable emulsion to below 30 °C, sequentially add niacinamide, sodium hyaluronate, ectoine, and azone, and adjust the pH to 5.8 - 6.2 with sodium citrate buffer solution to form the final product matrix;

[0072] S5. Add xanthan gum and carbomer to the end-product matrix, stir at 500 rpm at 25 °C until the viscosity reaches 3000 - 5000 mPa·s, and then filter through a 0.22 μm microporous membrane to obtain the composition.

[0073] Specifically, the preparation process of the Rosa roxburghii extract is as follows:

[0074] Mix the Rosa roxburghii fruit powder with 70% ethanol at a solid-liquid ratio of 1:10, extract at 40 - 50 °C under an ultrasonic power of 200 W for 30 minutes, filter, and then concentrate under reduced pressure until the solid content is ≥ 30% to obtain the Rosa roxburghii extract.

[0075] Specifically, the preparation parameters of the pyrroloquinoline quinone nanoliposomes are as follows:

[0076] Dissolve hydrogenated lecithin and cholesterol in a pH 7.4 phosphate buffer solution at a mass ratio of 4:1, add pyrroloquinoline quinone, and then transfer to a high-pressure homogenizer. Circulate 3 - 5 times under a pressure of 800 - 1000 bar. The particle size of the nanoliposomes is 80 - 120 nm, and the encapsulation efficiency is ≥ 85%.

[0077] Specifically, the preparation process of the French melon extract is as follows:

[0078] After the melon pulp is crushed, enzymolysis is carried out with cellulase with an enzyme activity of ≥ 5000 U / g for 2 - 4 hours at pH 6.5 - 7.0 and a temperature of 4 - 8 °C;

[0079] The enzymolysis solution is separated by a 50 kDa ultrafiltration membrane, and the active fragment with a molecular weight < 50 kDa is collected and freeze-dried to obtain the French melon extract.

[0080] Specifically, the emulsifier in step S3 is polysorbate-80, and the mass ratio is 0.5 g.

[0081] Specifically, the temperature in the primary emulsification stage in step S3 is 40 ± 1 °C, and the temperature is reduced to 35 ± 1 °C in the homogenization stage.

[0082] Specifically, the aqueous phase matrix includes 1,3-propanediol, and the oil phase matrix includes squalane.

[0083] Test method:

[0084] DPPH free radical scavenging rate: GB / T27599 - 2011;

[0085] Collagen synthesis: Cultivate human fibroblasts (HFF-1), and detect the secretion amount of type I collagen by ELISA method;

[0086] Skin moisturizing: 30 volunteers (aged 25 - 50) use it twice a day, and measure the TEWL value after 28 days.

[0087] The test results are shown in the following table:

[0088]

[0089]

[0090] Results: The DPPH free radical scavenging rate of Example 1 reached 92.5%, and cell experiments showed that the ATP production increased by ≥30%. Ergothioneine directly scavenges free radicals and activates the Nrf2 pathway to enhance the cell's own antioxidant capacity. Pyrroloquinoline quinone nano-liposomes (encapsulation efficiency ≥85%) improve the transdermal efficiency and enhance skin energy metabolism by promoting mitochondrial biogenesis. The French melon extract (high SOD activity) and the Rosa roxburghii extract (high vitamin C) form a "dual antioxidant system of enzymatic + non-enzymatic" to scavenge different types of free radicals (such as O 2 - ·, OH·);

[0091] In Example 1, the synthesis amount of type I collagen increased by 43.2%. Spermidine reduces collagen degradation through autophagy regulation and activates the collagen synthesis signaling pathway (such as TGF-β). Vitamin C in the Rosa roxburghii extract, as a cofactor of prolyl hydroxylase, directly promotes the cross-linking of collagen peptide chains. Pyrroloquinoline quinone PQQ indirectly protects the collagen synthesis microenvironment by reducing the damage of ROS to fibroblasts;

[0092] In Example 1, the TEWL value decreased by 27.9%, and clinical tests showed that the skin moisture content increased by 35%. Niacinamide enhances the synthesis of ceramide in the stratum corneum and repairs the lipid barrier; Ectoin forms a "molecular shield" to resist environmental stress (such as ultraviolet rays, pollutants); Sodium hyaluronate (low molecular weight) penetrates into the dermis to lock water and forms a long-acting moisturizing film in cooperation with squalane (oil phase).

[0093] In Example 2, due to the reduction in the dosage of ergothioneine, French melon extract, and PQQ, the antioxidant capacity decreased and mitochondrial activation was insufficient; the decrease in spermidine concentration weakened the autophagy regulation and collagen protection effects.

[0094] In Example 3, due to the reduction in the vitamin C content in the Rosa roxburghii extract, the synergistic antioxidant effect with SOD was weakened; the lack of addition of Ectoin led to insufficient environmental stress protection ability and limited improvement in TEWL.

[0095] In summary: Example 1 becomes the best solution through the following technical synergies:

[0096] Optimization of ingredient ratio: The concentrations of the core active ingredients (ergothioneine, PQQ, French melon extract) reach the synergistic threshold, achieving the effect of "1+1>2";

[0097] Process innovation:

[0098] Gradient temperature-controlled emulsification (40°C → 35°C) to avoid thermal degradation of spermidine and PQQ;

[0099] Nanoliposome encapsulation technology improves the transdermal rate and stability of PQQ (activity retention ≥ 90% after storage at 25°C for 6 months);

[0100] Functional complementary design:

[0101] Multi-dimensional anti-aging mechanism of antioxidant (ergothioneine + PQQ + Rosa roxburghii Tratt) + repair (spermidine + Ectoin) + moisturizing (sodium hyaluronate + squalane).

[0102] This composition can be adapted to various dosage forms such as essence, cream, and freeze-dried powder, meeting the composite requirements of anti-aging, repair, and moisturizing, and the preparation process can be mass-produced.

[0103] In addition, to provide a concise description of the exemplary embodiments, not all features of the actual embodiments may be described (i.e., those features that are not relevant to the currently considered best mode of carrying out the invention or those features that are not relevant to the implementation of the invention).

[0104] It should be understood that in the development of any actual implementation, as in any engineering or design project, a large number of specific implementation decisions may be made. Such development efforts may be complex and time-consuming, but for those of ordinary skill in the art who benefit from this disclosure, without excessive experimentation, such development efforts will be a routine task of design, manufacturing, and production.

[0105] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention, and they should all be covered by the scope of the claims of the present invention.

Claims

1. An ergothioneine anti-aging skin care composition, characterized in that: The formula contains the following components per 100g: ergothioneine 10-20mg, French melon extract 5-15mg, roxburghii extract 40-60mg, spermidine 3-8mg, pyrroloquinoline quinone nanoliposome 8-12mg, niacinamide 0.1-0.5g, sodium hyaluronate 0.05-0.2g, tetrahydromethylpyrimidine carboxylic acid 0.05-0.1g, xanthan gum 0.05-0.1g, carbomer 0.1-0.2g, and the remainder is a cosmetically acceptable matrix, including an aqueous phase and an oil phase.

2. A kind of ergothioneine anti-aging skin care composition according to claim 1, characterized in that: The formula contains the following components per 100g: 15mg of ergothioneine, 10mg of French melon extract, 50mg of sea buckthorn extract, 5mg of spermidine, 10mg of pyrroloquinoline quinone nanoliposomes, 0.3g of niacinamide, 0.1g of sodium hyaluronate, 0.08g of tetrahydromethylpyrimidine carboxylic acid, 0.08g of xanthan gum, and 0.1g of carbomer, and the remainder is a cosmetically acceptable matrix, including an aqueous phase and an oil phase.

3. according to the preparation method of a kind of ergothioneine anti-aging skin care composition described in any one of claim 1-2, it is characterized in that: The following steps are involved: S1, dissolving thioneine, French melon extract, and roxburghii extract in an aqueous phase matrix at a temperature of 35±2° C., stirring at 300 rpm for 10 minutes, adding spermidine, and treating under 40 kHz ultrasonic conditions until completely dissolved to obtain a spermidine-aqueous phase mixture; S2, slowly injecting the pyrroloquinoline quinone nanoliposomes into the spermidine-water phase mixture, stirring at 200-400 rpm for 20 minutes under light-proof conditions to form an active ingredient composite solution; S3, preheating the oil phase matrix to 40°C and then adding it to the active ingredient composite liquid with an emulsifier, emulsifying at a high speed of 10000-12000 rpm for 5 minutes in a vacuum emulsification tank with a vacuum degree of -0.08 MPa to form colostrum, transferring the colostrum to a high-pressure homogenizer, and circulating the treatment for 3 times at a pressure of 20 MPa to obtain a stable emulsion with a particle size of ≤5 μm; S4, cooling the stable emulsion to below 30°C, adding niacinamide, sodium hyaluronate, tetrahydromethylpyrimidine carboxylic acid and azone in sequence, and adjusting the pH to 5.8-6.2 with sodium citrate buffer to form a final product matrix; S5. Add xanthan gum and carbomer to the final product matrix, stir at 500 rpm at 25° C. until the viscosity reaches 3000-5000 mPa·s, and then filter through a 0.22 μm microporous filter membrane to obtain a composition.

4. The method for preparing the ergothioneine anti-aging skin care composition according to claim 3, wherein: The preparation process of the roxburghii extract is as follows: The roxburghii fruit powder and 70% ethanol were mixed in a solid-liquid ratio of 1:10, extracted for 30 minutes at 40-50° C. and an ultrasonic power of 200 W, filtered, and concentrated under reduced pressure to a solid content of ≥30% to obtain a roxburghii extract.

5. The method for preparing the ergothioneine anti-aging skin care composition according to claim 3, wherein: The preparation parameters of the pyrroloquinoline quinone nanoliposomes are: Hydrogenated lecithin and cholesterol were dissolved in a pH 7.4 phosphate buffer at a mass ratio of 4:1, and after adding pyrroloquinoline quinone, the mixture was transferred to a high-pressure homogenizer and circulated for 3-5 times at a pressure of 800-1000 bar. The particle size of the nanoliposomes was 80-120 nm, and the encapsulation efficiency was ≥85%.

6. The method for preparing the ergothioneine anti-aging skin care composition according to claim 3, wherein: The preparation process of the French cantaloupe extract is as follows: After the cantaloupe pulp is crushed, it is enzymatically hydrolyzed with cellulase having an enzyme activity of ≥5000U / g for 2-4 hours at pH 6.5-7.0 and temperature 4-8°C; The enzymatic hydrolysate was separated by a 50 kDa ultrafiltration membrane, and the active fragments with a molecular weight of less than 50 kDa were collected and freeze-dried to obtain the French melon extract.

7. The method for preparing the ergothioneine anti-aging skin care composition according to claim 3, wherein: The emulsifier in step S3 is polysorbate-80, with a mass ratio of 0.5-1 g.

8. The method for preparing the ergothioneine anti-aging skin care composition according to claim 3, wherein: In step S3, the temperature in the initial emulsification stage is 40±1°C, and the temperature is lowered to 35±1°C in the homogenization stage.

9. The method for preparing the ergothioneine anti-aging skin care composition according to claim 3, wherein: The aqueous phase matrix includes one of deionized water, glycerol, and 1,3-propylene glycol, and the oil phase matrix includes one of caprylic acid, capric triglyceride, squalane, and tocopherol acetate.

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