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Chufa polysaccharide and preparation method thereof

A technology of water chestnut polysaccharides and water chestnuts, applied in the field of water chestnut polysaccharides and its preparation, can solve the problems of little understanding of polysaccharide structures, unclear main active ingredients, unknown average molecular weight of water chestnut polysaccharides, etc.

Active Publication Date: 2015-11-18
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] 1. Water chestnuts are eaten as a whole. Because they contain many natural chemical components, the main active components are not clear, especially little is known about the polysaccharide structure contained in it.
[0011] 2. Since water chestnuts contain many natural components such as polysaccharides, flavonoids, polyphenols, vitamins, amino acids, and trace elements, only polysaccharides are natural high polymers. However, the average molecular weight of water chestnut polysaccharides is still unknown

Method used

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  • Chufa polysaccharide and preparation method thereof
  • Chufa polysaccharide and preparation method thereof
  • Chufa polysaccharide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1, a kind of preparation method of purifying water chestnut polysaccharide, carries out following steps successively:

[0069] 1) Purchase 2 kg of peeled water chestnuts, crush them in a juicer and squeeze the juice, then mix the juice and water chestnut residue evenly, add an appropriate amount of ultrapure water (about 6000g), heat at 80°C for 2 hours; then filter the juice with gauze , add appropriate amount of ultrapure water (about 3000g) to the filter residue, heat at 80°C for 1h, filter, combine the filtrates obtained from two filtrations to obtain water chestnut juice. Put the water chestnut juice in the refrigerator (4°C) overnight (about 10-12 hours), filter out the starch; pour the juice (about 9000ml) into an enamel plate and concentrate it to about 400-420ml to obtain the concentrated water chestnut juice.

[0070] 2), prepare sevage reagent (v / v of chloroform:n-butanol=5:1), the water chestnut juice after concentration is mixed with this reagent...

Embodiment 2

[0075] Embodiment 2, a kind of preparation method of purifying water chestnut polysaccharide, carries out following steps successively:

[0076] Step 1) is the same as step 1) of Embodiment 1;

[0077] 2), prepare sevage reagent (v / v of chloroform:n-butanol=5:1), water chestnut juice after concentration is mixed with this reagent by the volume ratio of 5:1, after stirring for 15min, centrifuge at 2000r / min for 10min, take upper liquid.

[0078] The supernatant liquid and 95% (volume %) ethanol were mixed according to the volume ratio of 1:3, stirred, stood overnight at a low temperature of 4°C, filtered, and the filtered precipitate was washed with petroleum ether (100ml) (removal was achieved during the washing process). fat), about 38g of water chestnut crude polysaccharide was obtained.

[0079] 3) Accurately weigh 0.5g of crude polysaccharide, dissolve it in 10ml of ultrapure water, centrifuge at a high speed (6000r / min for 10min), take the supernatant, add DEAE-52 cellu...

Embodiment 3

[0082] Embodiment 3, a kind of preparation method of purifying water chestnut polysaccharide, carries out following steps successively:

[0083] Step 1) is the same as step 1) of Embodiment 1;

[0084] 2), prepare sevage reagent (v / v of chloroform:n-butanol=5:1), water chestnut juice after concentration is mixed with this reagent by the volume ratio of 5:1, after stirring for 15min, centrifuge at 2000r / min for 60min, take upper liquid.

[0085] The supernatant liquid and 95% (volume %) ethanol are mixed according to the volume ratio of 1:10, stir, stand overnight at 4 ℃ of low temperature, filter, the precipitation after filtering is washed with sherwood oil (100ml) (realize to remove during washing process). fat), about 38g of water chestnut crude polysaccharide was obtained.

[0086] 3), accurately weigh 0.5g crude polysaccharide, dissolve it in 10ml ultrapure water, take the supernatant after 6000r / min high-speed centrifugation for 10min, add in the DEAE-52 cellulose chroma...

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Abstract

The invention discloses a chufa polysaccharide. The chufa polysaccharide consists of the following components in mol contents: 0.71% of mannose, 5% to 16% of rhamnose, 0.71% of galacturonic acid, 76.74% to 83.82% of glucose, 3.48% to 8.51% of galactose and 1.25% to 2.36% of arabinose. The Mn (number-average molecular weight) of the chufa polysaccharide is 1.116*10<7> to 1.137*10<7>, the Mw (weight-average molecular weight) of the chufa polysaccharide is 1.461*10<7> to 1.618*10<7>, and the polydispersity index of the chufa polysaccharide is 1.286 to 1.434. The chufa polysaccharide has better effects of preventing alcoholism and protecting the liver.

Description

technical field [0001] The invention relates to a preparation method and characterization of purified water chestnut polysaccharide. Background technique [0002] Water chestnut (Chinese Water Chestnut), also known as water chestnut, arrowhead mushroom, swallowtail grass, etc., is the bulb of the Cyperaceae water chestnut plant, and is loved by people because of its unique flavor. In addition to being eaten, water chestnuts also have certain medicinal value. They have the functions of lowering blood pressure, cooling blood and detoxification, diuresis and laxative, expectorant, digestion and swelling, and antibacterial. It has been documented that water chestnuts have a certain anti-alcoholic effect, and the development and application prospects are good. In recent years, studies have found that the polysaccharides in water chestnuts have good active functions. [0003] Water chestnuts have good medical and health care effects. According to the "Dictionary of Traditional Ch...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 葛建徐爱春林芳赵进胡华军邓同乐张永勇
Owner CHINA JILIANG UNIV
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