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Aromatic hydrocarbon degrading enzyme preparation and preparation method as well as application thereof

A technology for degrading enzymes and enzyme preparations, applied in the field of environmental microorganisms, can solve problems such as long cycles, destruction of habitat conditions, and changes, and achieve the effects of overcoming slow rates, improving bacterial metabolism efficiency, and economical sources

Inactive Publication Date: 2015-05-06
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, relying on microorganisms to metabolize organic pollutants for organic pollution treatment has the disadvantages of a long cycle and variable microbial communities. The succession of microbial communities makes it difficult for this method to ensure stable aromatics degradation capabilities; moreover, exogenous microbial species There is a potential ecological risk in the addition of , that is, changing the structure of the inherent microbial population and destroying the original habitat state

Method used

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  • Aromatic hydrocarbon degrading enzyme preparation and preparation method as well as application thereof
  • Aromatic hydrocarbon degrading enzyme preparation and preparation method as well as application thereof
  • Aromatic hydrocarbon degrading enzyme preparation and preparation method as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the preparation of aromatic hydrocarbon degrading enzyme agent and its degradation activity assay

[0025] 1. Preparation of aromatic hydrocarbon degrading enzyme

[0026] (1) Strain activation and seed liquid cultivation

[0027] The monoclonal colony of the aromatic hydrocarbon degrading strain was picked, added to 15ml of beef extract peptone culture medium, and cultured on a shaker at 30°C and 160rpm / min for 24h to obtain a seed solution. The degrading strain is Pseudomonas fluorescens PB4 (Pseudomonas fluorescens PB4), an indigenous functional microorganism, which was deposited in the China Center for Type Culture Collection on July 22, 2011, with a preservation number of CCTCC NO: M2011260. The address of the depository unit is Wuhan University, Wuhan, China.

[0028] (2) Fermentation culture of aromatic hydrocarbon degrading strains

[0029]Centrifuge the seed solution at 8000rpm / min for 5min to collect the thalli, wash the thalline twice with ph...

Embodiment 2

[0055] Example 2. Electrokinetic-Aromatics Degrading Enzyme Combined Remediation of Phenanthrene and Pyrene Contaminated Soil

[0056] Remediation of aromatics-contaminated soil by combining electrokinetic remediation with the high-efficiency aromatics degradation crude enzyme solution obtained in the above examples

[0057] In the test, a constant and continuous switching electric field was used to remediate the soil contaminated by phenanthrene and pyrene. The initial content of pyrene and phenanthrene in the contaminated soil were both 100ppm, the initial pH was 6.85, and the test area was 20×10×8cm 3 It was carried out in a polyvinyl chloride plastic box, and after 20 days of electrodynamic repair treatment, the aromatics efficient degradation crude enzyme solution obtained in the above examples was used for degradation treatment.

[0058] The test results showed that the removal rates of pyrene and phenanthrene in the soil after electrokinetic treatment (EK-35 d) for 35 d...

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Abstract

The invention relates to the technical field of environmental microorganisms, and particularly relates to an aromatic hydrocarbon degrading enzyme preparation and a preparation method as well as application thereof. The enzyme preparation is prepared by steps of fermenting and culturing the indigenous microorganism with an aromatic hydrocarbon degradation function, performing ultrasonication and extracting intracellular mixture, and further performing immobilization to form the enzyme preparation, wherein the indigenous microorganism with the aromatic hydrocarbon degradation function is pseudomonas fluorescens collected in the China Center for Type Culture Collection (CCTCC) with a collection number of CCTCC NO: M2011260 on July 22, 2011. In application, the enzyme preparation provided by the invention is efficient, fast, and free of secondary pollution relative to bacteria, so that a new biological remediation way and thinking is provided for treating the environment pollution caused by organic pollutants.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to an aromatic hydrocarbon degrading enzyme preparation and its preparation method and application. Background technique [0002] Aromatic hydrocarbons are hydrocarbons containing a benzene ring structure in the molecule. Because the soil environment is rich in organic matter, the lipophilicity of aromatic hydrocarbons makes them often adsorbed on the surface of soil particles, weakening their degradability; low molecular weight aromatic hydrocarbons These substances are volatile, have certain water solubility and poor lipophilicity, and are one of the main parts of water pollution. Research on remediation methods of aromatic hydrocarbon organic pollutants in soil and water has been widely carried out, including chemical remediation, biological remediation and chemical-biological joint remediation. In bioremediation, the research and application of microbe-enha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C02F3/34B09C1/10C12R1/39
Inventor 郭书海李凤梅王卅吴波
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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