Aromatic hydrocarbon degrading enzyme preparation and preparation method as well as application thereof
A technology for degrading enzymes and enzyme preparations, applied in the field of environmental microorganisms, can solve problems such as long cycles, destruction of habitat conditions, and changes, and achieve the effects of overcoming slow rates, improving bacterial metabolism efficiency, and economical sources
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Embodiment 1
[0024] Embodiment 1, the preparation of aromatic hydrocarbon degrading enzyme agent and its degradation activity assay
[0025] 1. Preparation of aromatic hydrocarbon degrading enzyme
[0026] (1) Strain activation and seed liquid cultivation
[0027] The monoclonal colony of the aromatic hydrocarbon degrading strain was picked, added to 15ml of beef extract peptone culture medium, and cultured on a shaker at 30°C and 160rpm / min for 24h to obtain a seed solution. The degrading strain is Pseudomonas fluorescens PB4 (Pseudomonas fluorescens PB4), an indigenous functional microorganism, which was deposited in the China Center for Type Culture Collection on July 22, 2011, with a preservation number of CCTCC NO: M2011260. The address of the depository unit is Wuhan University, Wuhan, China.
[0028] (2) Fermentation culture of aromatic hydrocarbon degrading strains
[0029]Centrifuge the seed solution at 8000rpm / min for 5min to collect the thalli, wash the thalline twice with ph...
Embodiment 2
[0055] Example 2. Electrokinetic-Aromatics Degrading Enzyme Combined Remediation of Phenanthrene and Pyrene Contaminated Soil
[0056] Remediation of aromatics-contaminated soil by combining electrokinetic remediation with the high-efficiency aromatics degradation crude enzyme solution obtained in the above examples
[0057] In the test, a constant and continuous switching electric field was used to remediate the soil contaminated by phenanthrene and pyrene. The initial content of pyrene and phenanthrene in the contaminated soil were both 100ppm, the initial pH was 6.85, and the test area was 20×10×8cm 3 It was carried out in a polyvinyl chloride plastic box, and after 20 days of electrodynamic repair treatment, the aromatics efficient degradation crude enzyme solution obtained in the above examples was used for degradation treatment.
[0058] The test results showed that the removal rates of pyrene and phenanthrene in the soil after electrokinetic treatment (EK-35 d) for 35 d...
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