Rice glume development gene promoter p-TRI1 and application thereof
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A promoter, transgenic rice technology, applied in the application, angiosperms/flowering plants, DNA/RNA fragments, etc., to achieve the effect of broad application and market prospects
Inactive Publication Date: 2010-08-25
CHINA AGRI UNIV
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[0006] Therefore, there are fewer researches on the mechanism of epiglume dev
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Embodiment 1
[0028] Example 1, Discovery of rice glume development gene promoter p-TRI1
[0029]1. Discovery of rice glume development gene TRI1
[0030] After 93-11 was treated with EMS (ethyl methane sulfonate) and self-crossed for multiple generations and its traits were observed, a mutant line with a pure genotype was formed. Through the screening of these mutant systems, a mutant with abnormal glume development was found. The specific performance of this mutant is: the glume width of the mutant is narrower than that of the normal wild type, that is, the glume width of 9311 is 2.84± 0.08mm, the glume width of the mutant was 2.33±0.11mm. However, there was no difference in the length of glumes between the two. The most obvious feature is that it exhibits a variation of triangular shape at its tip, hence the name tri1.
[0031] Tri1 was crossed with 93-11, Teqing, Guichao 2, Zhonghua 17, C418 and other conventional varieties respectively, and the hybrid F 1 It represents the characte...
Embodiment 2
[0043] Example 2, the acquisition and identification of p-TRI1 transgenic rice
[0044] 1. Construction of p-TRI1 plant expression vector
[0045] 1. Construction of p-LTT7 plant expression vector
[0046] 1. Using the genomic DNA of 93-11 rice as a template, use specific primers to T7EUS (T7EUSF / T7EUSR), and use the KODplus enzyme reaction kit of TOYOBO Biological Company to perform PCR amplification. After the reaction, perform PCR amplification. The amplified product was detected by 1% agarose gel electrophoresis, and the 2229bp DNA fragment (p-LTT7) was recovered and purified (the recovery kit used was the DNA product purification kit of Tiangen Biochemical Technology Co., Ltd., catalog number: DP204-02) .
[0047] 2. Digest the PCR product recovered in step 1 with restriction endonucleases EcoRI and SalI.
[0048] 3. Digest the plant expression vector pCambia1381 with restriction endonucleases EcoRI and SalI, and recover the vector skeleton.
[0049] 4. Ligate the dig...
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Abstract
The invention discloses a rice glume development gene promoter p-TRI1 and application thereof. The promoter provided by the invention comes from ordinary cultivated rice O. sativa L. 93-11 which belongs to Oryza species, and the promoter is a DNA molecule of the following 1), 2) or 3): 1) a DNA molecule shown in Sequence 1 in a sequence table; 2) a DNA molecule which crosses with a DNA sequence limited by 1) or 2) under strict condition and has the promoter function; and 3) a DNA molecule which has more than 90 percent of homology with the DNA sequence limited by 1) or 2) and has the promoter function. The discovery of the promoter and the elucidation of the promoter function have important theoretical and practical significance for studying flower organ development mechanism of rice, especially the glume development mechanism and for breeding rice varieties of specific grain types. The promoter has important theoretical and practical significance for studying the molecular mechanism of rice glume development and for rice grain type molecular breeding. The promoter has wide application and market prospect in the field of agricultural.
Description
technical field [0001] The invention relates to a rice glume development gene promoter p-TRI1 and application thereof. Background technique [0002] Rice is one of the most important food crops in the world, and it is also an ideal model plant for the study of monocot developmental biology. Rice flower organs are also the basis for the formation of food. The study of rice flower development has begun to become a new focus of plant molecular genetics. In recent years, the research on gene regulation of rice flower development has made great progress. [0003] Through the study of homeotic mutants of Arabidopsis thaliana and Antirrhinum thaliana, the ABC pattern of dicotyledonous plants was determined, that is, the floral organ of four rings is controlled by the interaction of three types of genes, which are respectively Called A, B, C class genes, each class of genes is responsible for regulating the formation of floral organs of two adjacent rings. Among them, class A ge...
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