Plants flower pesticide specificity promoter and uses thereof

Abstract

The invention discloses a plant anther specific promoter and the application. The promoter is the oligodeoxynucleotide with the length of 179bp to 1174bp. The 3'end of the promoter at least contains 996 site to 1174 site nucleic acid sequence at 5' end of sequence 1 in the self-sequence table, which extends along the nucleic acid arrangement mode of the sequence 1. The anther specific promoter has important application prospect for the function analysis and discrimination of the growth and development gene of the plant anther, the construction of artificial sterile system, the prevention of plant transgenic drift or escape and the extension of shelf life of flowers etc.

Description

Plant anther-specific promoter and its application technical field The invention relates to a plant anther-specific promoter and its application. Background technique Promoters (Promoters) are an integral part of a gene, controlling the initiation time and degree of gene expression (transcription). The promoter is like a "switch" that determines the activity of a gene. It basically determines whether, when and where a gene is expressed at the transcriptional level. According to the mode of action and function, promoters can be roughly divided into three categories: constitutive promoters, specific promoters and inducible promoters [Wang Guanlin, Fang Hongyun, 2002, Principles and Technology of Plant Genetic Engineering (Second Edition), Beijing, Science and Technology Press]. This classification basically reflects the respective functional characteristics of different types of promoters, but in some cases, one type of promoter often has the characteristics of other types...

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Problems solved by technology

Although the AtA7 gene was cloned as early as 1998 (MaH et al., 1998, PlantMolBiol, 37 (4): 607-619), and the anther specificity of its expression was also confirmed [YangSH et al., 2003, ThePlantCellVol.152792-2804; ColcombetJ et al., 2005, ThePlantCell, Vol.17, 3350-3361; YangSH et al., 2005, PlantPhysiology, 139 (1): 186-191], but no reports or patents have been seen for the research on its promoter. Whether anther tapetum-specific expression is the result of gene interactions or the regulation of promoter-specific elements remains unclear

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