Plant anther specific promoter and its application

Abstract

The invention discloses a plant anther-specific promoter and application thereof. The nucleotide sequence of the promoter is: (1), the nucleotide sequence described in Sequence 1 in the sequence listing; or (2), the nucleotide sequence complementary to the nucleotide sequence described in (1); Or (3), a nucleotide sequence having 60% or more homology with the nucleotide sequence described in (1) or (2); or (4), with (1), (2) or (3) The nucleotide sequence is a nucleotide sequence capable of hybridizing under stringent hybridization conditions. The expression vector containing the promoter and the antisense gene of the endogenous gene, the small RNA capable of interfering with the expression of the endogenous gene, or the fusion fragment of the exogenous gene can be used to transform the host cell or host tissue and its progeny cells to cultivate male sterile plants , Transgenic plants that can prevent transgenes from polluting wild species and related species through pollen drift, and landscaping plants or crop varieties that eliminate or greatly reduce "flying catkins".

Description

technical field [0001] The invention relates to a plant anther-specific promoter and its application. Background technique [0002] Promoter is the most important factor among gene expression regulators, which basically determines whether, when and where a gene is expressed. According to the mode of action and function, promoters can be roughly divided into three categories: constitutive promoters, specific promoters and inducible promoters [Wang Guanlin, Fang Hongyun, 2002, Principles and Technology of Plant Genetic Engineering (Second Edition), Beijing, Science and Technology Press]. This classification basically reflects the respective functional characteristics of different types of promoters, but in some cases, one type of promoter often has the characteristics of other types of promoters. [0003] An inducible promoter (inducible promoter) means that the gene controlled by it can greatly increase the transcription level under the stimulation of certain specific physi...

AI Technical Summary

Problems solved by technology

[0016] Although the use of anther-specific promoters to create genetically engineered male sterile lines and restorer lines has achieved great success, most rely on the anther-specific promoter TA29 from tobacco, other specific promoters, such as A9 (Worrall D et al., 1992, Plant Cell, 4:759-771; Paul et al., 1992), Anther-box (van der Meer et al., 1992), PS1 (Zuo JT et al., 1994, Amer J Bot, 81:552- 561), Osg6B [Tsuchiy T et al., 1995, Plant and Cell Physiol, 36 (3): 487-494; Matsuda N, 1996, Plant and Cell Physiol, 37 (2): 215-222; Kitashiba H et al., 1999, Mol Breeding, 5(2):209-218; Kawanab T et al., 2006, Plant and Cell Physiol, 47(6):784-787] and LAT52 [Muchietti J et al., 1994, Plant J, 6(3):321 -328], etc. have also been reported to cause male sterility, but most of them are not effective

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