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Rice glumes developmental gene AH1 and application thereof

A glume and gene technology, applied in the field of rice breeding, can solve the problem that the regulation mechanism of rice glume development is not completely clear.

Active Publication Date: 2019-03-12
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regulatory mechanism of glume development in rice is not yet fully understood

Method used

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  • Rice glumes developmental gene AH1 and application thereof
  • Rice glumes developmental gene AH1 and application thereof
  • Rice glumes developmental gene AH1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, the acquisition of mutant material

[0063] The japonica rice variety Nipponbare was chemically mutated by EMS, and the Nipponbare seeds to be treated were soaked in water for 8 hours, dried at room temperature, completely soaked in an EMS solution with a concentration of 1%, and treated for 10 hours at 28°C. The seeds are washed with tap water for 10-12 hours, and finally sowed for germination. Thus, a glume development mutant ah1 was screened. The traits of the mutant have been stably inherited through multiple generations of self-crossing. The observation and comparison of the mutant and the wild-type plant morphology throughout the growth cycle under field conditions showed a phenotype of curved lemma and degenerated lemma. All rice materials were planted in the experimental field of School of Chemistry, Zhejiang Normal University, Jinhua City, Zhejiang Province, under conventional management.

Embodiment 2

[0064] Embodiment 2, the phenotype analysis of plant

[0065] During the flowering stage, compared with the wild type Nipponbare, the spikelets of mutant ah1 had abnormal glume development, with curved lemma and degenerated palea, and the whole glume was crescent-shaped ( figure 1 ), the scanning electron microscope results of mutant and wild-type spikelets showed that the cells on the surface of the wild-type palea were evenly arranged, while the cells on the surface of the mutant ah1 palea were irregularly arranged ( figure 2 ).

Embodiment 3

[0066] Embodiment 3, population construction and genetic analysis

[0067] Mutant ah1 and regular indica TN 1 For cross mating, F 1 The plants showed normal wild-type phenotype, indicating that ah1 was controlled by a recessive nuclear gene. Statistics F 2 (F 1 Selfing) segregation population segregation ratio (table 1), the result shows, the segregation ratio of the plant of normal phenotype and the plant of mutant phenotype is close to 3:1 segregation through Chi-square test, and this shows that ah1 mutant is formed by a pair of single Recessive nuclear gene control.

[0068] Table 1 Genetic analysis of mutant ah1

[0069]

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Abstract

The invention belongs to the field of plant genetic engineering and particularly relates to control on isolation and cloning and function verification of a rice glumes developmental gene AH1 and application of the rice glumes developmental gene AH1 in rice breeding. The invention discloses the rice glumes developmental gene AH1. A nucleotide sequence of the gene is as shown in SEQ ID NO:2. The invention also simultaneously discloses a protein coded by the rice glumes developmental gene AH1. An nucleotide sequence of the protein is as shown in SEQ ID NO:4. By adopting the rice glumes developmental gene AH1, lemmas of glumes are bent, glumelle is degenerated, and the entire glumes is of a crescent shape; the rice glumes developmental gene AH1 can be used for improvement on yield and geneticquality of gramineous plants.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and in particular relates to the isolation and cloning of a gene AH1 for controlling glume development of rice, its function verification, and its application in rice breeding. Background technique [0002] Rice is one of the three major food crops in the world, and it is also a model organism for monocots. Flowering time, morphological structure of inflorescence and flower organ all have important influence on its yield and quality. The formation and development of floral organs is an important process of rice from vegetative growth to reproductive growth. The research on the morphology and development mechanism of floral organs will help to increase rice yield and improve its quality. Rice is a grass plant, and the spikelet is the structural unit of its inflorescence. A typical rice spikelet consists of a pair of glumes, a pair of glumes, a palea, a palea, two lodicules, six stamens an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C12N1/21C07K14/415A01H5/02A01H6/46
CPCC07K14/415C12N15/827
Inventor 饶玉春胡娟任德勇余海萍林晗章怡兰戴志俊林雪
Owner ZHEJIANG NORMAL UNIVERSITY
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