42results about How to "Regulate development" patented technology

The invention discloses a soil preparation. The preparation is prepared from soil subjected to sterilization treatment and probiotics or excipients which can be orally taken. The invention also discloses a preparation method of the soil preparation. The preparation method comprises the following steps: collecting pollution-free soil, performing autoclaved sterilization, cooling and then uniformly mixing with the probiotics or the excipients according to a predetermined proportion, thus obtaining capsules or electuary. The soil preparation prepared by the preparation method disclosed by the invention can be used for adjusting the structure and composition of a microbial of a human intestinal, stimulating the development of an immune system of a body and adjusting nervous development and neural activities, and achieving the aims of preventing and treating diseases. The raw materials adopted by the soil preparation are sufficient and are low in price; the preparation method is simple and is easy to operate.

The invention belongs to the field of feed additives, and particularly relates to a functional nutritive additive special for sows during the perinatal period, which can prevent constipation, shortenbirth process and promote colostrum secretion. The functional nutritive additive consists of the following components in parts by weight of 20-30 parts of an intestinal tract health regulator, 15-20 parts of a constitution regulator, 20-30 parts of colostrum secretion promoting regulator, 5-8 parts of carefully-chosen dietary fibers and 15-20 parts of glucose. The functional nutritive additive special for sows during the perinatal period has the functions of preventing constipation, shortening the birth process and promoting colostrum secretion; and besides, the functional nutritive additive special for sows during the perinatal period is low in production cost and suitable for large-scale popularization and application.

The invention discloses a novel cotton fiber secondary development specific expression gene GhNAC1 full-length sequence, and relates to an important cotton fiber development-associated regulator gene element. The GhNAC1 gene contains three exons and two introns, and is coded with an NAC (N Acetyl L Cysteine) transcription factor. A large quantity of mRNAs (messenger Ribonucleic Acids) of the gene are accumulated specifically at the secondary development stage of the cotton fiber cells, which indicates that the gene is a fiber secondary wall development specific gene. GhNAC1 protein is positioned in a cell nucleolus and has the capability of activating transcription independently, which indicates that the protein serving as a transcription activating factor plays a role in cotton fiber development. GhNAC1 is expressed excessively in arabidopsis, so that leaves crimp upwards, cell walls are thickened, ectopic sedimentation of lignin and the like is detected in foliar epidermic cells, so that the hemicellulose content of foliar cells is greatly increased. As proved by the results, the GhNAC1 can be used for regulating the biosynthesis of cell secondary walls, and plays a key role in the developing process of cotton fiber.

The invention discloses an auxin response factor gene PtARF3.1 for regulating and controlling adventitious root development of poplar and application thereof, and belongs to the technical field of biological genetic engineering. The nucleotide sequence of the PtARF3.1 is shown by SEQ ID NO:1 in a sequence table; the amino acid sequence of expression protein of the PtARF3.1 is shown by SEQ ID NO:2 in the sequence table; the nucleotide sequence of a carrier PMDC32-PtARF3.1 of the PtARF3.1 is shown by SEQ ID NO:3 in the sequence table. According to the invention, the PtARF3.1 gene is transferred to poplar; compared with wild type poplar, the transgenic poplar with overexpression PtARF3.1 has the advantages that the rooting is ahead of time, and the quantity of adventitious roots is increased, which suggest that the auxin response factor gene PtARF3.1 of poplar is a key regulatory factor for controlling adventitious root generation and development of poplar, and has important application value in the fields of forest-tree genetic engineering and clonal forest.

The invention relates to a walnut-tuckahoe oral liquid with function of providing assistance to improving memory and the production method thereof. The active ingredients in each 1000ml of the oral liquid comprise 100 to 180g of walnut kernel, 80 to 150g of rhizoma polygonati, 80 to 120g of tuckahoe, 80 to 150g of wheat, 30 to 60g of Chinese date, 4 to 6g of taurine, 1.5 to 2g of zinc gluconate, 0.05 to 0.1g of vitamin B1, 80 to 120g of white sugar and 0.2g of potassium sorbate. The oral liquid is prepared after procedures of extraction, condensation, ethanol precipitation, ethanol recovery, filtration, mixing, fine filtration, diluting to volume, encapsulation, sterilization, light inspection, packing, etc are carried out. The formula of the oral liquid is preferably selected according to the modern medical theory and treatment based on syndrome differentiation in the traditional Chinese medicine, thus the formula is scientific and reasonable, and the oral liquid can be conveniently taken, and is safe and reliable.

The invention discloses application of an ethylene responsive factor gene OsERF3 in regulating and controlling the development of rice roots. By using a yeast two-hybrid method, an ethylene responsive factor OsERF3 interacted with a published OsWOX11 protein is obtained by screening. By using an overexpression and small artificial interference RNA method, transformed rice corresponding to a gene is obtained, and a situation that the length of a main root and the number of crown roots of a plant subjected to gene overexpression are obviously increased in comparison with those of wild compared plants, and the length increasing of the main root is caused by the length increasing of cells in a maturation zone of a root tip, the length of the main root and the number of crown roots of a plant subjected to small artificial interference RNA transgenosis are obviously reduced in comparison with those of wild compared plants, and the length reducing of the main root is caused by the length reducing of cells in a meristematic zone and a maturation zone. Results show that the gene has a function of promoting the development of rice roots. A result of fusing the promoter of the gene with a report gene beta-glucuronidase shows that the gene is expressed in a meristematic zone of a root tip.

The invention provides a barley stripe disease pathogenic gene pgsln and an application thereof, belongs to the field of biotechnology engineering, and particularly provides a new gene pgsln derived from barley stripe disease sclerotinia sclerotiorum. The new gene pgsln can regulate and control the growth and development of barley streak pathogen filaments, mutants are knocked out through the pgsln gene, a large batch of mutant plants capable of resisting barley streak pathogens are cultivated, invasion of barley streak diseases can be efficiently solved, and the barley streak disease-resistant plants are simply, easily and efficiently cultivated. The new gene pgsln is related to osmotic stress and heavy metal stress regulation and control, and can improve the tolerance of barley stripe disease pathogenic bacteria plants to iprodione, carbendazim and fludioxonil and participate in the integrity of cell walls, i.e., the gene can regulate and control the coping capacity of the plants toadversity stress, and the pgsln transgenic crops are cultivated, so that the environment can be protected; and sustainable development of agriculture is facilitated.

The invention discloses an efficient composite carbon source for denitrification treatment of low-carbon-nitrogen-ratio wastewater and a preparation method thereof, and belongs to the field of water treatment. The preparation is prepared from, by weight, 10-25% of glucose, 3-5% of molasses, 1-5% of sodium citrate, 10-20% of glycerol, 3-5% of sodium formate, 3-8% of sodium acetate, 1-3% of inorganic phosphorus salt, 0.5-1% of microelements, 0.5-2% of growth factors and the balance water; the composite biological carbon source preparation disclosed by the invention can meet the nutrition required by microorganisms in different growth stages in a microbiota, realizes efficient denitrification of wastewater, and can improve the growth rate of the microbiota and the utilization rate of a carbonsource matrix by adding inorganic phosphorus salt, trace elements, growth factors and the like according to the quality of the wastewater, thereby further improving the biological denitrification efficiency.

The invention relates to a poplar leaf type developmental control gene and application thereof, which belong to the technical field of plant genetic engineering and the biological field. A nucleotidesequence of the poplar leaf type development control gene PagKNAT2 / 6b is as shown in a sequence 1 in a sequence table; an amino acid sequence, expressing protein, of PagKNAT2 / 6b is as shown in sequence 2 in the sequence table. The PagKNAT2 / 6b gene is shifted into 84k poplar, and the transgenic polar which excessively expresses PagKNAT2 / 6b is compared with a wild type, so that an obvious rolled andfolded leaf type appears, and therefore, the PagKNAT2 / 6b gene is the key of controlling poplar leaf development, and has an important application value in the woods genetic engineering field and theclonal forestry field.

The invention relates to schistosoma japonicum micro-ribonucleic acid (miRNA) and application thereof. Particularly, the invention discloses new miRNA which is separated out from schistosoma japonicum, wherein the miRNA can be specifically combined with a corresponding part of messenger RNA (mRNA) to influence the transcription effect of the corresponding mRNA so as to influence the expression of a corresponding gene. The invention also provides application of the identified schistosoma japonicum miRNA, a precursor sequence thereof, antisense oligonucleotide for adjusting the expression of the miRNA and the like in diagnosis, prevention and treatment of the schistosoma japonicum.

The invention relates to Japanese blood fluke miRNAs. In the invention, a new type of miRNAs is firstly separated from blood flukes, and the expression of the miRNAs has the characteristic of specificity in the life history of blood flukes. Antisense oligonucleotides of MiRNAs modified by cercaria transfection in the infective stage of blood flukes can affect the subsequent growth of blood flukes. The invention provides approved Japanese blood fluke miRNAs and a precursor sequence thereof, and applications of antisense oligonucleotides and the like for regulating the expression of miRNAs in prevention and control of blood flukes.

The invention discloses a laying duck follicle growth-associated miRNA alpha-mir-145 and detecting primer, inhibitor and application thereof and belongs to the technical field of genetic engineering.The nucleotide sequence of the laying duck follicle growth-associated miRNA alpha-mir-145 is shown as SED ID NO: 1. Research shows that the laying duck follicle growth-associated miRNA alpha-mir-145 can regulate follicle growth through targeted PIK3CA genes, reduce expression of a cell proliferation marker gene CyclinB2 and enhance expression of a cell apoptosis marker gene BCL2. The laying duck follicle growth-associated miRNA alpha-mir-145 can serve as a marker for detecting proliferation of laying duck follicular cells, thereby filling the gap in the field, providing a theoretical basis anda scientific basis for analysis on the genetic mechanism of laying duck follicle growth and being of great significance to researching the genetic nature of duck reproduction traits, improving the reproductivity of laying ducks and achieving assisted genetic breeding.

The invention relates to the technical field of plant molecular biology, in particular to application of a wheat WLSH1 gene in regulation and control of spike and grain development of plants. It is found that the WLHS1 gene has the function of regulating and controlling wheat spike and grain development, and can regulate and control the number of spikelets per spike of wheat and the number of grains per spike of wheat. Overexpression of the WLHS1 gene in wheat can significantly increase the number of spikelets per spike of wheat and the number of grains per spike of wheat. Thereby, a new thought is provided for increasing the yield of wheat.

The invention provides a culture method for in-vitro human oocyte preantral follicles. The culture method is characterized by comprising the following specific steps of: 1) unfreezing the in-vitro human oocytes placed in frozen protecting solution containing cane sugar and propylene glycol and subjected to liquid nitrogen low-temperature storage, and then reviving and culturing; and 2) separating and extracting the in-vitro human oocytes obtained by reviving and culturing, then placing the in-vitro human oocytes in nutrient solution containing follicle-stimulating hormone (FSH), and performing preantral culture, wherein the culture for in-vitro human oocyte preantral follicles needs the support of FSH, and FSH can be used for promoting the growth of the follicles, increasing the survival time of the follicles, promoting the proliferation of granular cells, and promoting the maturation of the oocytes, as well as is an important factor for regulating and controlling the growth and development of the follicles; and trichostatin A can be used for effectively inhibiting the proliferation of tumour cells, regulating and controlling the progress of a cell cycle and then inducing the apoptosis of tumour cells, thus ensuring the survival quality of the cells. The method has important significance on researches in the aspect of test-tube babies.

The present invention applies for a full-price compound feed for beef cattle fattening. The full-price compound feed comprises a 187 granule material or powdered material, 188 granule material or powdered material and a 189 granule material or powdered material; the 187 granule material or powdered material, the 188 granule material or powdered material and the 189 granule material or powdered material all consist of corn, soybean meal, corn germ mash, sprayed corn husk, distiller's grains, corn husk, hay, wheat middling, barley husk, soybean husk, stone powder, glucose, glutamic acid residues, bentonite, sodium chloride and a cattle premix. Beneficial effects are as follows: the full-price compound feed for the beef cattle fattening is the mixed granule feed respectively aiming at calves, growing cattle and fattening cattle for feeding, and plays the functions of regulating the rumen development of the calves, improving the immunity and survival of the calves, improving the rumen development of the growing cattle, cultivating bones, increasing body size and laying a good foundation for fattening. Besides, the 189 granule material or powder material focuses on improving the rumennutrients of the feed, improves feed conversion efficiency and growth efficiency, shortens feeding cycles and reduces farming costs.

The invention discloses a plant glandular hair specific expression gene HD-9 as well as an expression vector and application thereof, and aims to solve the technical problem of lack of plant glandular hair head secretory cell development regulation means at present. The gene HD-9 capable of regulating and controlling development of glandular hair cells is identified, an overexpression vector and a CRISPR / Cas9 vector of the gene are constructed, and a corresponding overexpression strain and a gene knockout strain are obtained through conversion respectively. Phenotype and physiological feature identification and detection show that: the HD-9 gene can regulate and control the development of glandular head cells of plant long-stem glandular hair, and has important application value in the aspects of regulating and increasing the content of glandular hair secretions, directionally improving glandular hair types and the like.

The invention discloses application of an OsRL3.3 protein to regulation and control of plant root development. The OsRL3.3 protein is a protein shown as a) or b) or c) or d), wherein a) is a protein of which the amino acid sequence is shown as a SEQ ID NO: sequence 2; (b) is a fused protein obtained by connecting a tag to the terminal N and / or terminal C of the protein shown as the SEQ ID NO: sequence 2; c) is a protein which has the same function and is obtained by performing substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence shown as the SEQ ID NO: 2; d) has the same function and has 75 percent or more of homology with the amino acid sequence shown as the SEQ ID NO: sequence 2. As proved by experiments, the normal development of therice root system can be influenced by the OsRL3.3 gene mutation. The gene has the function of regulating and controlling the development of rice roots and primary roots, and can be applied to the improvement of the characters of the rice root system.

The invention relates to a walnut-tuckahoe oral liquid with function of providing assistance to improving memory and the production method thereof. The active ingredients in each 1000ml of the oral liquid comprise 100 to 180g of walnut kernel, 80 to 150g of rhizoma polygonati, 80 to 120g of tuckahoe, 80 to 150g of wheat, 30 to 60g of Chinese date, 4 to 6g of taurine, 1.5 to 2g of zinc gluconate, 0.05 to 0.1g of vitamin B1, 80 to 120g of white sugar and 0.2g of potassium sorbate. The oral liquid is prepared after procedures of extraction, condensation, ethanol precipitation, ethanol recovery, filtration, mixing, fine filtration, diluting to volume, encapsulation, sterilization, light inspection, packing, etc are carried out. The formula of the oral liquid is preferably selected according tothe modern medical theory and treatment based on syndrome differentiation in the traditional Chinese medicine, thus the formula is scientific and reasonable, and the oral liquid can be conveniently taken, and is safe and reliable.

The invention discloses a virus-free potato cutting seedling planting method. The method at least comprises steps as follows: 1) a 20 cm thick substrate is paved with river sand with the size of 0.5-1mm on a seedling bed, virus-free potato cutting seedlings are transplanted, and root systems of the virus-free potato cutting seedlings are buried into the substrate; 2) after transplantation, the cutting seedlings are covered with a plastic film and a sunshade gauze, the plastic film is removed step by step after the seedlings recover for 7-10 days and the cutting seedlings grow new roots in thesubstrate, and the sunshade gauze is removed after 30-40 days of transplantation; 3) water-soluble mixed fertilizer is applied after 9-11 days of transplantation; and 4) after the potato fruiting period begins, the maximum water-holding capacity of the substrate is controlled at 75%-80%, the temperature of the growth environment is controlled at 17 DEGC-25 DEGC, and diseases, insect pest and weeds are controlled on the potatoes in the whole growth process. According to the method, the river sand is paved on the seedling bed to serve as the substrate, the roots of the cutting seedlings are buried in the substrate layer, and virus infection in the growth process of the cutting seedlings is effectively reduced.

The invention belongs to the technical field of veterinary medicines, and particularly discloses a functional medicine having the effect of reproduction regulation. Each 100 parts of the functional medicine is characterized by consisting of the following raw materials in parts by weight: 4-6 parts of dihydropyridine, 1-2 parts of a basil essential oil compound, 0.4-0.6 part of a traditional Chinese medicine extract, and the balance of auxiliary materials. The functional medicine having the effect of reproduction regulation, provided by the invention, is applied to a feed additive for livestockand poultry, the defects caused by a previous method of injecting hormone are overcome, the propagation function obstacle of animals can be effectively solved, besides, the reproductive function, thehormone level and the genital development of animal bodies can be regulated, the breeding and childbirth properties of livestock and poultry are increased, and the breeding benefits are effectively increased.

The invention discloses the application of OsRL3.3 protein in regulating plant root development. The OsRL3.3 protein is the protein of a) or b) or c) or d) as follows: a) the amino acid sequence is the protein shown in sequence 2; b) it is connected at the N-terminal and / or C-terminal of the protein shown in sequence 2 The fusion protein obtained by labeling; c) the protein with the same function obtained by substituting and / or deleting and / or adding the amino acid sequence shown in sequence 2 through one or several amino acid residues; d) the same function as shown in sequence 2 Proteins with 75% or more homology in amino acid sequence and the same function. It is proved by experiments that the mutation of OsRL3.3 gene can affect the normal development of rice root system, the gene has the function of regulating the development of rice root and primary root, and can be used to improve the traits of rice root system.

The invention discloses a method for renewing old randia cochinchinensis trees, and belongs to the technical field of agricultural planting. The method comprises the following steps: (1) fertilizing; (2) retracting for several times; (3) fixing of tips; (4) pruning; (5) water and fertilizer management; and (6) disease and pest control. The method for renewing the old randia cochinchinensis trees is simple, labor-saving, fast in growth, easy in crown formation, capable of promoting early fruiting and capable of reducing cost, by means of the method, the crowns of the old randia cochinchinensis tree orchard are renewed and rejuvenated, soil fertility is enhanced, diseases and pests of the old randia cochinchinensis trees are controlled in time, the production capacity of the old randia cochinchinensis trees is effectively improved, rapid renewal and rejuvenation are achieved, the yield and quality of the old randia cochinchinensis trees are improved, high and stable yield in successive years is achieved, the high-quality fruit rate is increased, and sustainable development of randia cochinchinensis is realized.

The invention relates to a poplar leaf type developmental control gene and application thereof, which belong to the technical field of plant genetic engineering and the biological field. A nucleotidesequence of the poplar leaf type development control gene PagKNAT2 / 6b is as shown in a sequence 1 in a sequence table; an amino acid sequence, expressing protein, of PagKNAT2 / 6b is as shown in sequence 2 in the sequence table. The PagKNAT2 / 6b gene is shifted into 84k poplar, and the transgenic polar which excessively expresses PagKNAT2 / 6b is compared with a wild type, so that an obvious rolled andfolded leaf type appears, and therefore, the PagKNAT2 / 6b gene is the key of controlling poplar leaf development, and has an important application value in the woods genetic engineering field and theclonal forestry field.

The invention describes the application of the sesame protein SiBRB in regulating the development of plant roots. By overexpressing the gene encoding the sesame protein SiBRB in Arabidopsis, it was found that this gene can significantly reduce the total lateral root length and root fresh weight of Arabidopsis plants, and finally reduce the total root system. This shows that the gene is a key gene that limits the growth of plant roots, and has a good application prospect in regulating plant root development and regulating plant root-to-shoot ratio.

The invention discloses a laying duck follicle growth-associated miRNA alpha-mir-145 and detecting primer, inhibitor and application thereof and belongs to the technical field of genetic engineering.The nucleotide sequence of the laying duck follicle growth-associated miRNA alpha-mir-145 is shown as SED ID NO: 1. Research shows that the laying duck follicle growth-associated miRNA alpha-mir-145 can regulate follicle growth through targeted PIK3CA genes, reduce expression of a cell proliferation marker gene CyclinB2 and enhance expression of a cell apoptosis marker gene BCL2. The laying duck follicle growth-associated miRNA alpha-mir-145 can serve as a marker for detecting proliferation of laying duck follicular cells, thereby filling the gap in the field, providing a theoretical basis anda scientific basis for analysis on the genetic mechanism of laying duck follicle growth and being of great significance to researching the genetic nature of duck reproduction traits, improving the reproductivity of laying ducks and achieving assisted genetic breeding.

The invention discloses a key gene PeSCE1 for controlling formation of adventitious roots of poplars and plant height development and application of the key gene PeSCE1, wherein the nucleotide sequence of the key gene PeSCE1 is shown as SEQ ID NO.1, and the amino acid sequence of an expression protein of the key gene PeSCE1 is shown as SEQ ID NO.2. The invention further discloses application of the key gene PeSCE1. The method comprises the following steps: cloning a PeSCE1 gene through an RACE technology by taking adventitious roots of poplar Nanlin 895 as a material, and constructing an overexpression vector 35S:: PeSCE1 by utilizing a Gateway technology, and transferring the overexpression vector 35S:: PeSCE1 into the poplar. The number of adventitious roots of the PeSCE1 transgenic poplar is obviously reduced, and the plant height is also obviously reduced. Therefore, the PeSCE1 gene is a key gene for regulating and controlling formation of adventitious roots and plant height development of poplars, and has important application value in the fields of forest genetic engineering and clone forestry.

The invention relates to an auxin receptor gene for regulating the development of adventitious roots of poplar and its application, belonging to the fields of plant genetic engineering and biotechnology. The nucleotide sequence of PtrFBL1 is shown in sequence 1 in the sequence listing; the amino acid sequence of the expressed protein of PtrFBL1 is shown in sequence 2 in the sequence listing. The present invention transfers the PtrFBL1 gene into 84k poplar, and compared with the wild type, the transgenic poplar overexpressing PtrFBL1 obviously takes root earlier, and the number of adventitious roots increases significantly, the total length of adventitious roots and the total area of ​​adventitious roots increase significantly, indicating that the PtrFBL1 gene regulates poplar The key regulator gene of adventitious root development has important application value in the field of tree genetic engineering and clonal forestry.