42results about How to "Regulate development" patented technology

The invention discloses a soil preparation. The preparation is prepared from soil subjected to sterilization treatment and probiotics or excipients which can be orally taken. The invention also discloses a preparation method of the soil preparation. The preparation method comprises the following steps: collecting pollution-free soil, performing autoclaved sterilization, cooling and then uniformly mixing with the probiotics or the excipients according to a predetermined proportion, thus obtaining capsules or electuary. The soil preparation prepared by the preparation method disclosed by the invention can be used for adjusting the structure and composition of a microbial of a human intestinal, stimulating the development of an immune system of a body and adjusting nervous development and neural activities, and achieving the aims of preventing and treating diseases. The raw materials adopted by the soil preparation are sufficient and are low in price; the preparation method is simple and is easy to operate.

The invention belongs to the field of feed additives, and particularly relates to a functional nutritive additive special for sows during the perinatal period, which can prevent constipation, shortenbirth process and promote colostrum secretion. The functional nutritive additive consists of the following components in parts by weight of 20-30 parts of an intestinal tract health regulator, 15-20 parts of a constitution regulator, 20-30 parts of colostrum secretion promoting regulator, 5-8 parts of carefully-chosen dietary fibers and 15-20 parts of glucose. The functional nutritive additive special for sows during the perinatal period has the functions of preventing constipation, shortening the birth process and promoting colostrum secretion; and besides, the functional nutritive additive special for sows during the perinatal period is low in production cost and suitable for large-scale popularization and application.

The invention relates to a walnut-tuckahoe oral liquid with function of providing assistance to improving memory and the production method thereof. The active ingredients in each 1000ml of the oral liquid comprise 100 to 180g of walnut kernel, 80 to 150g of rhizoma polygonati, 80 to 120g of tuckahoe, 80 to 150g of wheat, 30 to 60g of Chinese date, 4 to 6g of taurine, 1.5 to 2g of zinc gluconate, 0.05 to 0.1g of vitamin B1, 80 to 120g of white sugar and 0.2g of potassium sorbate. The oral liquid is prepared after procedures of extraction, condensation, ethanol precipitation, ethanol recovery, filtration, mixing, fine filtration, diluting to volume, encapsulation, sterilization, light inspection, packing, etc are carried out. The formula of the oral liquid is preferably selected according to the modern medical theory and treatment based on syndrome differentiation in the traditional Chinese medicine, thus the formula is scientific and reasonable, and the oral liquid can be conveniently taken, and is safe and reliable.

The invention provides a barley stripe disease pathogenic gene pgsln and an application thereof, belongs to the field of biotechnology engineering, and particularly provides a new gene pgsln derived from barley stripe disease sclerotinia sclerotiorum. The new gene pgsln can regulate and control the growth and development of barley streak pathogen filaments, mutants are knocked out through the pgsln gene, a large batch of mutant plants capable of resisting barley streak pathogens are cultivated, invasion of barley streak diseases can be efficiently solved, and the barley streak disease-resistant plants are simply, easily and efficiently cultivated. The new gene pgsln is related to osmotic stress and heavy metal stress regulation and control, and can improve the tolerance of barley stripe disease pathogenic bacteria plants to iprodione, carbendazim and fludioxonil and participate in the integrity of cell walls, i.e., the gene can regulate and control the coping capacity of the plants toadversity stress, and the pgsln transgenic crops are cultivated, so that the environment can be protected; and sustainable development of agriculture is facilitated.

The invention discloses an efficient composite carbon source for denitrification treatment of low-carbon-nitrogen-ratio wastewater and a preparation method thereof, and belongs to the field of water treatment. The preparation is prepared from, by weight, 10-25% of glucose, 3-5% of molasses, 1-5% of sodium citrate, 10-20% of glycerol, 3-5% of sodium formate, 3-8% of sodium acetate, 1-3% of inorganic phosphorus salt, 0.5-1% of microelements, 0.5-2% of growth factors and the balance water; the composite biological carbon source preparation disclosed by the invention can meet the nutrition required by microorganisms in different growth stages in a microbiota, realizes efficient denitrification of wastewater, and can improve the growth rate of the microbiota and the utilization rate of a carbonsource matrix by adding inorganic phosphorus salt, trace elements, growth factors and the like according to the quality of the wastewater, thereby further improving the biological denitrification efficiency.

The invention relates to schistosoma japonicum micro-ribonucleic acid (miRNA) and application thereof. Particularly, the invention discloses new miRNA which is separated out from schistosoma japonicum, wherein the miRNA can be specifically combined with a corresponding part of messenger RNA (mRNA) to influence the transcription effect of the corresponding mRNA so as to influence the expression of a corresponding gene. The invention also provides application of the identified schistosoma japonicum miRNA, a precursor sequence thereof, antisense oligonucleotide for adjusting the expression of the miRNA and the like in diagnosis, prevention and treatment of the schistosoma japonicum.

The invention relates to Japanese blood fluke miRNAs. In the invention, a new type of miRNAs is firstly separated from blood flukes, and the expression of the miRNAs has the characteristic of specificity in the life history of blood flukes. Antisense oligonucleotides of MiRNAs modified by cercaria transfection in the infective stage of blood flukes can affect the subsequent growth of blood flukes. The invention provides approved Japanese blood fluke miRNAs and a precursor sequence thereof, and applications of antisense oligonucleotides and the like for regulating the expression of miRNAs in prevention and control of blood flukes.

The invention discloses a laying duck follicle growth-associated miRNA alpha-mir-145 and detecting primer, inhibitor and application thereof and belongs to the technical field of genetic engineering.The nucleotide sequence of the laying duck follicle growth-associated miRNA alpha-mir-145 is shown as SED ID NO: 1. Research shows that the laying duck follicle growth-associated miRNA alpha-mir-145 can regulate follicle growth through targeted PIK3CA genes, reduce expression of a cell proliferation marker gene CyclinB2 and enhance expression of a cell apoptosis marker gene BCL2. The laying duck follicle growth-associated miRNA alpha-mir-145 can serve as a marker for detecting proliferation of laying duck follicular cells, thereby filling the gap in the field, providing a theoretical basis anda scientific basis for analysis on the genetic mechanism of laying duck follicle growth and being of great significance to researching the genetic nature of duck reproduction traits, improving the reproductivity of laying ducks and achieving assisted genetic breeding.

The invention relates to the technical field of plant molecular biology, in particular to application of a wheat WLSH1 gene in regulation and control of spike and grain development of plants. It is found that the WLHS1 gene has the function of regulating and controlling wheat spike and grain development, and can regulate and control the number of spikelets per spike of wheat and the number of grains per spike of wheat. Overexpression of the WLHS1 gene in wheat can significantly increase the number of spikelets per spike of wheat and the number of grains per spike of wheat. Thereby, a new thought is provided for increasing the yield of wheat.

The invention provides a culture method for in-vitro human oocyte preantral follicles. The culture method is characterized by comprising the following specific steps of: 1) unfreezing the in-vitro human oocytes placed in frozen protecting solution containing cane sugar and propylene glycol and subjected to liquid nitrogen low-temperature storage, and then reviving and culturing; and 2) separating and extracting the in-vitro human oocytes obtained by reviving and culturing, then placing the in-vitro human oocytes in nutrient solution containing follicle-stimulating hormone (FSH), and performing preantral culture, wherein the culture for in-vitro human oocyte preantral follicles needs the support of FSH, and FSH can be used for promoting the growth of the follicles, increasing the survival time of the follicles, promoting the proliferation of granular cells, and promoting the maturation of the oocytes, as well as is an important factor for regulating and controlling the growth and development of the follicles; and trichostatin A can be used for effectively inhibiting the proliferation of tumour cells, regulating and controlling the progress of a cell cycle and then inducing the apoptosis of tumour cells, thus ensuring the survival quality of the cells. The method has important significance on researches in the aspect of test-tube babies.

The invention discloses a virus-free potato cutting seedling planting method. The method at least comprises steps as follows: 1) a 20 cm thick substrate is paved with river sand with the size of 0.5-1mm on a seedling bed, virus-free potato cutting seedlings are transplanted, and root systems of the virus-free potato cutting seedlings are buried into the substrate; 2) after transplantation, the cutting seedlings are covered with a plastic film and a sunshade gauze, the plastic film is removed step by step after the seedlings recover for 7-10 days and the cutting seedlings grow new roots in thesubstrate, and the sunshade gauze is removed after 30-40 days of transplantation; 3) water-soluble mixed fertilizer is applied after 9-11 days of transplantation; and 4) after the potato fruiting period begins, the maximum water-holding capacity of the substrate is controlled at 75%-80%, the temperature of the growth environment is controlled at 17 DEGC-25 DEGC, and diseases, insect pest and weeds are controlled on the potatoes in the whole growth process. According to the method, the river sand is paved on the seedling bed to serve as the substrate, the roots of the cutting seedlings are buried in the substrate layer, and virus infection in the growth process of the cutting seedlings is effectively reduced.

The invention belongs to the technical field of veterinary medicines, and particularly discloses a functional medicine having the effect of reproduction regulation. Each 100 parts of the functional medicine is characterized by consisting of the following raw materials in parts by weight: 4-6 parts of dihydropyridine, 1-2 parts of a basil essential oil compound, 0.4-0.6 part of a traditional Chinese medicine extract, and the balance of auxiliary materials. The functional medicine having the effect of reproduction regulation, provided by the invention, is applied to a feed additive for livestockand poultry, the defects caused by a previous method of injecting hormone are overcome, the propagation function obstacle of animals can be effectively solved, besides, the reproductive function, thehormone level and the genital development of animal bodies can be regulated, the breeding and childbirth properties of livestock and poultry are increased, and the breeding benefits are effectively increased.

The invention discloses a method for renewing old randia cochinchinensis trees, and belongs to the technical field of agricultural planting. The method comprises the following steps: (1) fertilizing; (2) retracting for several times; (3) fixing of tips; (4) pruning; (5) water and fertilizer management; and (6) disease and pest control. The method for renewing the old randia cochinchinensis trees is simple, labor-saving, fast in growth, easy in crown formation, capable of promoting early fruiting and capable of reducing cost, by means of the method, the crowns of the old randia cochinchinensis tree orchard are renewed and rejuvenated, soil fertility is enhanced, diseases and pests of the old randia cochinchinensis trees are controlled in time, the production capacity of the old randia cochinchinensis trees is effectively improved, rapid renewal and rejuvenation are achieved, the yield and quality of the old randia cochinchinensis trees are improved, high and stable yield in successive years is achieved, the high-quality fruit rate is increased, and sustainable development of randia cochinchinensis is realized.

The invention discloses a key gene PeSCE1 for controlling formation of adventitious roots of poplars and plant height development and application of the key gene PeSCE1, wherein the nucleotide sequence of the key gene PeSCE1 is shown as SEQ ID NO.1, and the amino acid sequence of an expression protein of the key gene PeSCE1 is shown as SEQ ID NO.2. The invention further discloses application of the key gene PeSCE1. The method comprises the following steps: cloning a PeSCE1 gene through an RACE technology by taking adventitious roots of poplar Nanlin 895 as a material, and constructing an overexpression vector 35S:: PeSCE1 by utilizing a Gateway technology, and transferring the overexpression vector 35S:: PeSCE1 into the poplar. The number of adventitious roots of the PeSCE1 transgenic poplar is obviously reduced, and the plant height is also obviously reduced. Therefore, the PeSCE1 gene is a key gene for regulating and controlling formation of adventitious roots and plant height development of poplars, and has important application value in the fields of forest genetic engineering and clone forestry.