Cloning and identification of cotton fiber cell secondary wall development-associated GhNAC1 gene

A gene and gene coding technology, applied in genetic engineering, plant genetic improvement, fermentation, etc., can solve problems such as affecting the assembly of wall components and affecting content.

Inactive Publication Date: 2012-04-04
HUAZHONG NORMAL UNIV
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Problems solved by technology

The above studies also show that a well-defined transcription factor network regulates the synthesis of plant secondary walls, and affects the content of some proteins related to cell wall development, thereby affecting the assembly of wall components

Method used

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  • Cloning and identification of cotton fiber cell secondary wall development-associated GhNAC1 gene
  • Cloning and identification of cotton fiber cell secondary wall development-associated GhNAC1 gene
  • Cloning and identification of cotton fiber cell secondary wall development-associated GhNAC1 gene

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Embodiment Construction

[0033] Cloning identification and functional analysis of the full-length sequence of a new cotton NAC family gene GhANC1:

[0034] 1. Isolation and identification of the full-length sequence of GhNAC1 and its cDNA sequence

[0035] More than 10,000 cotton cDNA clones were isolated from the cotton fiber cDNA library for DNA sequencing. Through bioinformatics analysis, the full-length cDNA sequences of 10 GhNAC genes were obtained, one of which was named GhNAC1. Primers GhNAC1 OEup and GhNAC1 OEdn were designed according to the sequence of GhNAC1 cDNA, and the full-length DNA sequence of the gene was amplified by PCR technology using cotton genome DNA as a template.

[0036] 2. Quantitative RT-PCR analysis of GhNAC1 gene expression

[0037] (1) Extraction of total RNA from cotton tissue (according to Li XB, Cai L, Cheng NH, Liu JW, 2002. Molecular characterization of the cotton GhTUB1 gene that preferentially expressed in fiber. Plant Physiol. 130: 666-674).

[0038] (2) Real...

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Abstract

The invention discloses a novel cotton fiber secondary development specific expression gene GhNAC1 full-length sequence, and relates to an important cotton fiber development-associated regulator gene element. The GhNAC1 gene contains three exons and two introns, and is coded with an NAC (N Acetyl L Cysteine) transcription factor. A large quantity of mRNAs (messenger Ribonucleic Acids) of the gene are accumulated specifically at the secondary development stage of the cotton fiber cells, which indicates that the gene is a fiber secondary wall development specific gene. GhNAC1 protein is positioned in a cell nucleolus and has the capability of activating transcription independently, which indicates that the protein serving as a transcription activating factor plays a role in cotton fiber development. GhNAC1 is expressed excessively in arabidopsis, so that leaves crimp upwards, cell walls are thickened, ectopic sedimentation of lignin and the like is detected in foliar epidermic cells, so that the hemicellulose content of foliar cells is greatly increased. As proved by the results, the GhNAC1 can be used for regulating the biosynthesis of cell secondary walls, and plays a key role in the developing process of cotton fiber.

Description

Technical field: [0001] The present invention relates to cotton genes. Specifically, the cloning and functional identification of a NAC transcription factor (NAC transcription factor) gene GhNAC1 specifically expressed in the secondary wall of cotton fiber cells. Background technique: [0002] Cotton is an important economic crop in my country, which is closely related to the income of 200 million farmers and the employment of nearly 19 million textile workers in China. With the rapid development of the textile industry and the continuous improvement of people's living standards, the textile industry and its related industries have higher and higher requirements for the quality of cotton fibers. my country's total lint production ranks first in the world, and its per unit yield also ranks first among the five major cotton-producing countries. However, the quality of my country's cotton fibers is poor and cannot be used as raw materials for the production of some high-value-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415
Inventor 黄耿青李学宝李鹏张杰李雯
Owner HUAZHONG NORMAL UNIV
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